BLASTX 1.5.4-Paracel [2003-06-05]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= At5g21000.F
(456 letters)
Database: BlastDB/NCBI/blast/db/FASTA/2004_07_04_01_00_0/nr
1,895,298 sequences; 629,712,918 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_213726.1| cysteinyl-tRNA synthetase [Aquifex aeolicu... 36 0.13
ref|XP_397634.1| hypothetical protein UM00019.1 [Ustilago m... 32 3.2
ref|NP_440810.1| nitrate transport protein; NrtC [Synechocy... 31 5.5
gb|AAH56081.1| MGC69072 protein [Xenopus laevis] 30 7.2
ref|ZP_00156239.1| COG0350: Methylated DNA-protein cysteine... 30 9.5
pir||T14558 RNA polymerase homolog - beet mitochondrion|gi|... 30 9.5
ref|NP_704890.1| hypothetical protein [Plasmodium falciparu... 30 9.5
>ref|NP_213726.1| cysteinyl-tRNA synthetase [Aquifex aeolicus
VF5]|gi|6831683|sp|O67163|SYC_AQUAE Cysteinyl-tRNA
synthetase (Cysteine--tRNA ligase)
(CysRS)|gi|7437719|pir||H70391 cysteine-tRNA ligase (EC
6.1.1.16) - Aquifex aeolicus|gi|2983551|gb|AAC07125.1|
cysteinyl-tRNA synthetase [Aquifex aeolicus VF5]
Length = 495
Score = 36.2 bits (82), Expect = 0.13
Identities = 22/70 (31%), Positives = 37/70 (52%), Gaps = 3/70 (4%)
Frame = +1
Query: 184 DEEEGFGRSLIVIDITTDYLPHLKAVALFIVSLLKFEKKLKSAAEKQCSEI--IASVY-G 354
D G GRSLIV D+ +L HL F+ + + K+ + A+++C++ IA Y
Sbjct: 36 DSHVGHGRSLIVFDVFRRFLEHLGYQVKFVRNFTDVDDKIINRAKQECTDFMTIADRYIA 95
Query: 355 EIQLELENDR 384
+++EN R
Sbjct: 96 RYYVDMENIR 105
>ref|XP_397634.1| hypothetical protein UM00019.1 [Ustilago maydis
521]|gi|46096171|gb|EAK81404.1| hypothetical protein
UM00019.1 [Ustilago maydis 521]
Length = 709
Score = 31.6 bits (70), Expect = 3.2
Identities = 15/65 (23%), Positives = 27/65 (41%)
Frame = +1
Query: 145 ISKGIYSTQSFQMDEEEGFGRSLIVIDITTDYLPHLKAVALFIVSLLKFEKKLKSAAEKQ 324
+S G Y + F+ +E R I+ PHL L + + K + A E++
Sbjct: 271 LSGGTYESSGFEHKKESTIARESFFIEFAERIRPHLSTARLAVTGGFRSSKAMAKAVEER 330
Query: 325 CSEII 339
+I+
Sbjct: 331 SCDIV 335
>ref|NP_440810.1| nitrate transport protein; NrtC [Synechocystis sp. PCC
6803]|gi|2492543|sp|P73450|NRTC_SYNY3 Nitrate transport
ATP-binding protein nrtC|gi|7445984|pir||S77387 nitrate
transport protein C-2 - Synechocystis sp. (strain PCC
6803)|gi|1652569|dbj|BAA17490.1| nitrate transport
protein; NrtC [Synechocystis sp. PCC 6803]
Length = 670
Score = 30.8 bits (68), Expect = 5.5
Identities = 21/65 (32%), Positives = 33/65 (50%), Gaps = 3/65 (4%)
Frame = -2
Query: 236 SVVISITIRDLPKPSSSSIWKD*VLYIPLEINEAGLNTRRL---MPLRLAVAASLSSRPM 66
++ ++ +RDLPKP I D + + L+ A L M R+A+A +LS+RP
Sbjct: 100 ALAVNRVLRDLPKPEQEKIIDDNIALVGLQ-RAAHKRPGELSGGMKQRVAIARALSTRPK 158
Query: 65 SFSLD 51
LD
Sbjct: 159 VLLLD 163
>gb|AAH56081.1| MGC69072 protein [Xenopus laevis]
Length = 435
Score = 30.4 bits (67), Expect = 7.2
Identities = 26/117 (22%), Positives = 47/117 (39%)
Frame = +1
Query: 55 NEKLIGLEEREAATANLKGIRRLVLSPASLISKGIYSTQSFQMDEEEGFGRSLIVIDITT 234
N++ I + E N LV+ S+I +G+YS
Sbjct: 105 NQRRISVSESAFQDGNFS----LVIKDVSMIDQGLYSCNLHH------------------ 142
Query: 235 DYLPHLKAVALFIVSLLKFEKKLKSAAEKQCSEIIASVYGEIQLELENDRSVYTDRY 405
+ HL +++ K E+K+K + + I+A V+ + L EN ++TDR+
Sbjct: 143 -HYCHLDETVRVQLNITKSERKVKIYWDGEKIVIVALVHSTVLLPCENHDHMWTDRH 198
>ref|ZP_00156239.1| COG0350: Methylated DNA-protein cysteine methyltransferase
[Haemophilus influenzae R2866]
Length = 167
Score = 30.0 bits (66), Expect = 9.5
Identities = 11/26 (42%), Positives = 20/26 (76%)
Frame = -3
Query: 211 ETFQSLPLHPSGKTEYYISLWKSMRQ 134
E F ++PL P G TE+ +S+W+++R+
Sbjct: 54 ERFSNIPLKPEG-TEFQLSIWQALRE 78
>pir||T14558 RNA polymerase homolog - beet
mitochondrion|gi|2832369|emb|CAA71809.1| RNA polymerase
[Beta vulgaris subsp. vulgaris]
Length = 1014
Score = 30.0 bits (66), Expect = 9.5
Identities = 27/88 (30%), Positives = 46/88 (51%), Gaps = 2/88 (2%)
Frame = +1
Query: 133 PASLISKG-IYSTQSFQMDEEEGFGRSLIVIDITTDYLPHLKAVALFIVSLLKFEKKLKS 309
PA L +G IY + F E + RSL++ + T++YL K +LF L+ E S
Sbjct: 525 PAFLDFRGRIYRSGIFHFHERD-LARSLLLFEKTSNYLDEKKMDSLFDKYLIATEFLYSS 583
Query: 310 -AAEKQCSEIIASVYGEIQLELENDRSV 390
++EK E++ S Y E + ++ + + V
Sbjct: 584 YSSEKNLPEVMHS-YAEKKCQISDPKLV 610
>ref|NP_704890.1| hypothetical protein [Plasmodium falciparum
3D7]|gi|23505253|emb|CAD52033.1| hypothetical protein
[Plasmodium falciparum 3D7]
Length = 713
Score = 30.0 bits (66), Expect = 9.5
Identities = 29/124 (23%), Positives = 48/124 (38%), Gaps = 4/124 (3%)
Frame = +1
Query: 49 LSNEKLIGLEEREAATANLKG----IRRLVLSPASLISKGIYSTQSFQMDEEEGFGRSLI 216
L E I +E +A TA K ++ AS ++ T+ + E+ +
Sbjct: 345 LYEELQIVYDENQAKTAQAKDEQHELKPQAEKDASKLTTTYDQTKEVKPQAEKDASKLTT 404
Query: 217 VIDITTDYLPHLKAVALFIVSLLKFEKKLKSAAEKQCSEIIASVYGEIQLELENDRSVYT 396
D T + P + A + + K++K AEK + GE+ + E D S T
Sbjct: 405 TYDQTKEVKPQAEKDASKLTTTYDQTKEVKPQAEKDALAKTENQNGELLPQAEKDASKLT 464
Query: 397 DRYD 408
YD
Sbjct: 465 TTYD 468
Database: BlastDB/NCBI/blast/db/FASTA/2004_07_04_01_00_0/nr
Posted date: Jul 5, 2004 10:54 PM
Number of letters in database: 629,712,918
Number of sequences in database: 1,895,298
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 629,712,918
effective HSP length: 127
effective length of database: 389,010,072
effective search space used: 9336241728
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)