BLASTX 1.5.4-Paracel [2003-06-05]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= At4g03735.F
         (342 letters)

Database: BlastDB/NCBI/blast/db/FASTA/2004_07_04_01_00_0/nr 
           1,895,298 sequences; 629,712,918 total letters

Searching...................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_740931.2|  cation Diffusion Facilitator family member...    37   0.12 
emb|CAE70090.1|  Hypothetical protein CBG16532 [Caenorhabdit...    35   0.35 
emb|CAE64126.1|  Hypothetical protein CBG08742 [Caenorhabdit...    35   0.35 
ref|NP_688541.1|  glycosyl transferase, group 2 family prote...    35   0.45 
ref|NP_703960.1|  putative DNA polymerase epsilon, catalytic...    33   1.3  
ref|NP_671842.1|  hypothetical protein [Arabidopsis thaliana]      32   2.9  
ref|NP_009145.1|  SEC63-like protein; SEC63, endoplasmic ret...    31   5.0  
ref|XP_234266.2|  similar to mitogen-activated protein kinas...    31   5.0  
pir||T46504  hypothetical protein DKFZp434M179.1 - human (fr...    31   5.0  
ref|NP_008370.1|COX1_13186  cytochrome c oxidase subunit I [...    31   5.0  
>ref|NP_740931.2| cation Diffusion Facilitator family member (82.3 kD) (cdf-5)
           [Caenorhabditis elegans]|gi|24817580|emb|CAD21657.2|
           Hypothetical protein Y105E8A.3 [Caenorhabditis elegans]
          Length = 746

 Score = 36.6 bits (83), Expect = 0.12
 Identities = 28/96 (29%), Positives = 39/96 (40%), Gaps = 10/96 (10%)
 Frame = -2

Query: 290 SSVGSIGYFSGVTMPV----------FKASPSCFQSRTTFVGLNFNGSSVWCFVTSISIA 141
           SS  SIGYF    + V          F A   CFQ     V      SSV  F  +  +A
Sbjct: 255 SSGASIGYFHYAALLVIIAVFVFVLDFYAESICFQHVADPVMAAARWSSVTMFSCAFGLA 314

Query: 140 FLWIGLSALVAQSISRNIAFGVFCVVQGAFSLVCFN 33
           +LW G   L   +++  ++  V C +  + SL   N
Sbjct: 315 YLWYGSQNLGDHALTGGVSITVVCFILASISLTHSN 350
>emb|CAE70090.1| Hypothetical protein CBG16532 [Caenorhabditis briggsae]
          Length = 666

 Score = 35.0 bits (79), Expect = 0.35
 Identities = 33/97 (34%), Positives = 41/97 (42%), Gaps = 8/97 (8%)
 Frame = +1

Query: 22  TMITLKQTREKAPWTTQNTPNAMLREIDCATRADKPIQRKAIEIEVTKHQ--TD------ 177
           T I+ + T E  P++  N      +E     R +K   R  I IEVTK Q  TD      
Sbjct: 124 TQISFQSTIENKPYSYHNEEERQTQEQRAFGRLNK--LRGLISIEVTKSQVFTDISSNIF 181

Query: 178 EPLKFKPTNVVLDWKQLGLALNTGIVTPEKYPIEPTE 288
           EPL   P+N      Q G   NT  V  EK  +E  E
Sbjct: 182 EPLSSTPSNKAHALTQTGKECNTEEVQTEKGEMEDEE 218
>emb|CAE64126.1| Hypothetical protein CBG08742 [Caenorhabditis briggsae]
          Length = 743

 Score = 35.0 bits (79), Expect = 0.35
 Identities = 26/96 (27%), Positives = 40/96 (41%), Gaps = 10/96 (10%)
 Frame = -2

Query: 290 SSVGSIGYFSGVTMPV----------FKASPSCFQSRTTFVGLNFNGSSVWCFVTSISIA 141
           SS+ S+G+F    + V          F A   CFQ     V      SSV  F  +  +A
Sbjct: 255 SSLASMGFFEYAILLVVVAVFVFVLDFYAESICFQHVADPVMAAARWSSVTMFSCAFGLA 314

Query: 140 FLWIGLSALVAQSISRNIAFGVFCVVQGAFSLVCFN 33
           +LW G   L   +++  ++  V C +  + SL   N
Sbjct: 315 YLWYGHQNLGDHALTGGVSITVVCFILASISLTHSN 350
>ref|NP_688541.1| glycosyl transferase, group 2 family protein [Streptococcus
           agalactiae 2603V/R]|gi|22534578|gb|AAN00414.1| glycosyl
           transferase, group 2 family protein [Streptococcus
           agalactiae 2603V/R]
          Length = 422

 Score = 34.7 bits (78), Expect = 0.45
 Identities = 22/93 (23%), Positives = 45/93 (47%), Gaps = 3/93 (3%)
 Frame = -2

Query: 287 SVGSIGYFSGVTMPVFKASPSCFQSRTTFVGLNFNGSSVWCFVTSISIAFLW---IGLSA 117
           +VGSI Y   +   +      CF     F+ L FN  ++  F++ +S+   +   I LS+
Sbjct: 293 AVGSISYIYYILFELLTPFIECFGIVIIFLSLLFNQLNIPFFISLVSLYIFYCVLITLSS 352

Query: 116 LVAQSISRNIAFGVFCVVQGAFSLVCFNVIMVH 18
            + +  S+ +  G+  +V+  F +  F  +++H
Sbjct: 353 FLHRIYSQQLVIGILDIVK-VFYIAVFRYLILH 384
>ref|NP_703960.1| putative DNA polymerase epsilon, catalytic subunit a [Plasmodium
            falciparum 3D7]|gi|46361254|emb|CAG25115.1| DNA
            polymerase epsilon, catalytic subunit a, putative;
            putative DNA polymerase epsilon, catalytic subunit a
            [Plasmodium falciparum 3D7]
          Length = 2907

 Score = 33.1 bits (74), Expect = 1.3
 Identities = 23/95 (24%), Positives = 42/95 (44%), Gaps = 3/95 (3%)
 Frame = +1

Query: 58   PWTTQNTPNAMLREIDCATRADKPIQRKAIEIEVTKHQTDEPLK---FKPTNVVLDWKQL 228
            P T+ + P+ + ++I+ +    K I    ++  + K  T  PL     K  N + D+K  
Sbjct: 1291 PITSISVPDWLKKQINISEGKQKKITSFFVKKNIKKEDTTSPLHSADVKLVNDIHDFKDS 1350

Query: 229  GLALNTGIVTPEKYPIEPTEEISDIGKENPEKFPV 333
                NT +   +K  ++  E IS+   E    FP+
Sbjct: 1351 NETTNTPLHYTQKESMKNKEHISNQSMEKKSPFPI 1385
>ref|NP_671842.1| hypothetical protein [Arabidopsis thaliana]
          Length = 147

 Score = 32.0 bits (71), Expect = 2.9
 Identities = 12/19 (63%), Positives = 17/19 (89%)
 Frame = +3

Query: 285 RRNIRHRQREPGEISGEPM 341
           RR+IR++Q EPGE+S EP+
Sbjct: 60  RRSIRYKQMEPGEVSDEPL 78
>ref|NP_009145.1| SEC63-like protein; SEC63, endoplasmic reticulum translocon
           component (S. cerevisiae) like [Homo
           sapiens]|gi|18203500|sp|Q9UGP8|SC63_HUMAN Translocation
           protein SEC63 homolog|gi|3978517|gb|AAC83375.1| SEC63
           [Homo sapiens]|gi|5327054|emb|CAB46275.1| SEC63 protein
           [Homo sapiens]|gi|28502837|gb|AAH47221.1| SEC63-like
           protein [Homo sapiens]
          Length = 760

 Score = 31.2 bits (69), Expect = 5.0
 Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
 Frame = +1

Query: 154 EVTKHQTDEPLKFKPTNVVLDW-KQLGLALNTGIVTPEKYPIEPTEEISDIGKENPEK 324
           +  K +  +PLK KPT V+L   KQ       G+V  E    E  EE+SD G ++ E+
Sbjct: 522 KTAKSKKKKPLKKKPTPVLLPQSKQQKQKQANGVVGNEAAVKEDEEEVSDKGSDSEEE 579
>ref|XP_234266.2| similar to mitogen-activated protein kinase kinase kinase kinase 5
           [Rattus norvegicus]
          Length = 1988

 Score = 31.2 bits (69), Expect = 5.0
 Identities = 27/99 (27%), Positives = 41/99 (41%), Gaps = 13/99 (13%)
 Frame = +1

Query: 64  TTQNTPNAMLREIDCATRADKPIQRKAIEIEVTKHQTDEPLK-----------FKPTNVV 210
           ++ + P+A++R    +T  +   +R A EI   K Q + PL+               N +
Sbjct: 674 SSADKPHAIIRHTIRSTNRNSRAERTASEINFDKLQFEPPLRKETEARDEMGLSSEPNFI 733

Query: 211 LDWKQLGLALNTGIVTPEKYPIE--PTEEISDIGKENPE 321
           L W       NTG +    YP E  P EE S   K  P+
Sbjct: 734 LHWNPFVDGANTGRI--NSYPEESLPDEEKSSTVKRCPD 770
>pir||T46504 hypothetical protein DKFZp434M179.1 - human
           (fragment)|gi|6807848|emb|CAB70701.1| hypothetical
           protein [Homo sapiens]
          Length = 632

 Score = 31.2 bits (69), Expect = 5.0
 Identities = 21/58 (36%), Positives = 30/58 (51%), Gaps = 1/58 (1%)
 Frame = +1

Query: 154 EVTKHQTDEPLKFKPTNVVLDW-KQLGLALNTGIVTPEKYPIEPTEEISDIGKENPEK 324
           +  K +  +PLK KPT V+L   KQ       G+V  E    E  EE+SD G ++ E+
Sbjct: 394 KTAKSKKKKPLKKKPTPVLLPQSKQQKQKQANGVVGNEAAVKEDEEEVSDKGSDSEEE 451
>ref|NP_008370.1|COX1_13186 cytochrome c oxidase subunit I [Onchocerca
           volvulus]|gi|7432820|pir||T11065 cytochrome-c oxidase
           (EC 1.9.3.1) chain I - nematode  (Onchocerca volvulus)
           mitochondrion|gi|2735937|gb|AAC61612.1| cytochrome
           oxidase subunit 1 [Onchocerca volvulus]
          Length = 548

 Score = 31.2 bits (69), Expect = 5.0
 Identities = 22/64 (34%), Positives = 29/64 (44%), Gaps = 3/64 (4%)
 Frame = -2

Query: 269 YFSGVTMPVFKASPSC---FQSRTTFVGLNFNGSSVWCFVTSISIAFLWIGLSALVAQSI 99
           YFS  TM +  A PS    F    TF G N     +WC+  S    F   GLS ++  + 
Sbjct: 314 YFSAATMII--AIPSAVKIFNWLGTFFGSNQKMQPLWCWTYSFIFLFTVGGLSGIILSAA 371

Query: 98  SRNI 87
           S +I
Sbjct: 372 SLDI 375
  Database: BlastDB/NCBI/blast/db/FASTA/2004_07_04_01_00_0/nr
    Posted date:  Jul 5, 2004 10:54 PM
  Number of letters in database: 629,712,918
  Number of sequences in database:  1,895,298
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 629,712,918
effective HSP length: 89
effective length of database: 461,031,396
effective search space used: 11064753504
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)







BLAST Search Results