BLASTX 1.5.4-Paracel [2003-06-05]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AU236010_RAFL14_59_B19.f
(544 letters)
Database: BlastDB/NCBI/blast/db/FASTA/2004_07_04_01_00_0/nr
1,895,298 sequences; 629,712,918 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||D86476 protein F15O4.43 [imported] - Arabidopsis thali... 160 2e-47
emb|CAE68195.1| Hypothetical protein CBG13856 [Caenorhabdit... 39 0.072
ref|XP_392964.1| similar to ENSANGP00000012035 [Apis mellif... 33 3.0
emb|CAG59971.1| unnamed protein product [Candida glabrata] 33 3.9
gb|AAK59515.1| unknown protein [Arabidopsis thaliana]|gi|23... 32 6.7
ref|NP_974035.1| expressed protein [Arabidopsis thaliana] 32 6.7
gb|AAL04415.1| zonadhesin splice variant 2 [Homo sapiens] 32 8.8
ref|NP_775080.1| zonadhesin isoform 4 [Homo sapiens]|gi|126... 32 8.8
ref|NP_775081.1| zonadhesin isoform 5 [Homo sapiens]|gi|126... 32 8.8
ref|NP_003377.1| zonadhesin isoform 3 [Homo sapiens]|gi|126... 32 8.8
>pir||D86476 protein F15O4.43 [imported] - Arabidopsis
thaliana|gi|8778359|gb|AAF79367.1| F15O4.43 [Arabidopsis
thaliana]
Length = 352
Score = 160 bits (406), Expect(2) = 2e-47
Identities = 79/81 (97%), Positives = 80/81 (98%)
Frame = +1
Query: 76 REKASDLLFPATIASLVFVGQFSVQNLMAFPAWMSGFCPAMFRATFDCRGSSDTVRFFTT 255
+EKASDLLFPATIASLVFVGQFSVQNLMAFPAWMSGFCPAMFRATFDCRGSSDTVRFFTT
Sbjct: 93 KEKASDLLFPATIASLVFVGQFSVQNLMAFPAWMSGFCPAMFRATFDCRGSSDTVRFFTT 152
Query: 256 MVGLRLASIGFFSGGRLAKIS 318
MVGLRLASIGFFSGGRLAK S
Sbjct: 153 MVGLRLASIGFFSGGRLAKTS 173
Score = 50.8 bits (120), Expect(2) = 2e-47
Identities = 31/65 (47%), Positives = 33/65 (50%)
Frame = +2
Query: 311 RFQESIAFGRSFLSKFANEVWWIL*LKINGPSLEAPAVSRWFLEFPEFANQNGGEWRQLR 490
RFQESIAFGRSFLSKFA N+NGGEWRQLR
Sbjct: 196 RFQESIAFGRSFLSKFA--------------------------------NENGGEWRQLR 223
Query: 491 LLQAT 505
LLQ+T
Sbjct: 224 LLQST 228
>emb|CAE68195.1| Hypothetical protein CBG13856 [Caenorhabditis briggsae]
Length = 1555
Score = 38.5 bits (88), Expect = 0.072
Identities = 22/81 (27%), Positives = 41/81 (50%)
Frame = -3
Query: 293 LKKPMEAKRKPTIVVKNRTVSEEPRQSNVALNIAGQKPDIHAGKAIRFWTENWPTNTKEA 114
L+K E R T K+R + ++ +++N AL ++ D + +R WTE W +
Sbjct: 1248 LRKADEELRDQT--AKSRNLEQQLKEANKALTTMNKEKDRAVERVVREWTERWNVERHQV 1305
Query: 113 IVAGKRRSDAFSL*KMQMREE 51
+A R ++A S + M+E+
Sbjct: 1306 QLAQNRTAEAESKVRQLMKEK 1326
>ref|XP_392964.1| similar to ENSANGP00000012035 [Apis mellifera]
Length = 5803
Score = 33.1 bits (74), Expect = 3.0
Identities = 20/58 (34%), Positives = 32/58 (54%), Gaps = 4/58 (6%)
Frame = -3
Query: 338 SQRQLIPEILASLPPLK----KPMEAKRKPTIVVKNRTVSEEPRQSNVALNIAGQKPD 177
S + P I+ +P LK K +E R P+ + T S+EP + + +L+IAG+ PD
Sbjct: 4482 SDKSKSPSIIGDVPDLKDVDAKGVEKSRSPSAT--SVTESKEPLEKSKSLSIAGEAPD 4537
>emb|CAG59971.1| unnamed protein product [Candida glabrata]
Length = 2238
Score = 32.7 bits (73), Expect = 3.9
Identities = 20/59 (33%), Positives = 30/59 (49%)
Frame = -1
Query: 259 PSS*RTEQCQKNHDNQTLP*TSPDKSQTSMLEKP*DSGQRTGQQTLKKLLLPEKEDQML 83
P++ RT + DN TLP S +Q +E D Q+T T+KK E++D +L
Sbjct: 554 PANVRTSDNTASQDNDTLPSESVKSNQQQNVESSSDR-QQTSADTVKKFSFLEEDDDLL 611
>gb|AAK59515.1| unknown protein [Arabidopsis thaliana]|gi|23296532|gb|AAN13120.1|
unknown protein [Arabidopsis thaliana]
Length = 108
Score = 32.0 bits (71), Expect = 6.7
Identities = 24/71 (33%), Positives = 34/71 (47%), Gaps = 4/71 (5%)
Frame = +2
Query: 308 LRFQESIAFGRSFLSKFANEVWWIL*LKINGPSL-EAPAVSRWFLEFPEFANQ---NGGE 475
L F ++FGRSFL KF ++V W +K S+ + AVS F F N+ NG E
Sbjct: 38 LEFGNQLSFGRSFLCKFDDDVCWDSLIKNRWTSVFKVLAVSGGFGVFRLLLNRSAMNGDE 97
Query: 476 WRQLRLLQATC 508
+ + C
Sbjct: 98 ESSIHVFNFIC 108
>ref|NP_974035.1| expressed protein [Arabidopsis thaliana]
Length = 91
Score = 32.0 bits (71), Expect = 6.7
Identities = 24/71 (33%), Positives = 34/71 (47%), Gaps = 4/71 (5%)
Frame = +2
Query: 308 LRFQESIAFGRSFLSKFANEVWWIL*LKINGPSL-EAPAVSRWFLEFPEFANQ---NGGE 475
L F ++FGRSFL KF ++V W +K S+ + AVS F F N+ NG E
Sbjct: 21 LEFGNQLSFGRSFLCKFDDDVCWDSLIKNRWTSVFKVLAVSGGFGVFRLLLNRSAMNGDE 80
Query: 476 WRQLRLLQATC 508
+ + C
Sbjct: 81 ESSIHVFNFIC 91
>gb|AAL04415.1| zonadhesin splice variant 2 [Homo sapiens]
Length = 2689
Score = 31.6 bits (70), Expect = 8.8
Identities = 26/91 (28%), Positives = 41/91 (44%), Gaps = 1/91 (1%)
Frame = -3
Query: 296 PLKKPMEAKRKPTIVVKNRTVS-EEPRQSNVALNIAGQKPDIHAGKAIRFWTENWPTNTK 120
P +KP KPTI + T+S EEP I+ +KP I K TE T+ +
Sbjct: 776 PTEKPTIPTEKPTIPTEKPTISTEEPTTPTEETTISTEKPSIPMEKP-TLPTEETTTSVE 834
Query: 119 EAIVAGKRRSDAFSL*KMQMREEREKPTKPS 27
E ++ ++ ++ + EKPT P+
Sbjct: 835 ETTIS----TEKLTIPMEKPTISTEKPTIPT 861
>ref|NP_775080.1| zonadhesin isoform 4 [Homo sapiens]|gi|12667416|gb|AAK01434.1|
zonadhesin variant 4 [Homo sapiens]
Length = 2624
Score = 31.6 bits (70), Expect = 8.8
Identities = 26/91 (28%), Positives = 41/91 (44%), Gaps = 1/91 (1%)
Frame = -3
Query: 296 PLKKPMEAKRKPTIVVKNRTVS-EEPRQSNVALNIAGQKPDIHAGKAIRFWTENWPTNTK 120
P +KP KPTI + T+S EEP I+ +KP I K TE T+ +
Sbjct: 776 PTEKPTIPTEKPTIPTEKPTISTEEPTTPTEETTISTEKPSIPMEKP-TLPTEETTTSVE 834
Query: 119 EAIVAGKRRSDAFSL*KMQMREEREKPTKPS 27
E ++ ++ ++ + EKPT P+
Sbjct: 835 ETTIS----TEKLTIPMEKPTISTEKPTIPT 861
>ref|NP_775081.1| zonadhesin isoform 5 [Homo sapiens]|gi|12667418|gb|AAK01435.1|
zonadhesin variant 5 [Homo sapiens]
Length = 2601
Score = 31.6 bits (70), Expect = 8.8
Identities = 26/91 (28%), Positives = 41/91 (44%), Gaps = 1/91 (1%)
Frame = -3
Query: 296 PLKKPMEAKRKPTIVVKNRTVS-EEPRQSNVALNIAGQKPDIHAGKAIRFWTENWPTNTK 120
P +KP KPTI + T+S EEP I+ +KP I K TE T+ +
Sbjct: 776 PTEKPTIPTEKPTIPTEKPTISTEEPTTPTEETTISTEKPSIPMEKP-TLPTEETTTSVE 834
Query: 119 EAIVAGKRRSDAFSL*KMQMREEREKPTKPS 27
E ++ ++ ++ + EKPT P+
Sbjct: 835 ETTIS----TEKLTIPMEKPTISTEKPTIPT 861
>ref|NP_003377.1| zonadhesin isoform 3 [Homo sapiens]|gi|12667414|gb|AAK01433.1|
zonadhesin variant 3 [Homo sapiens]
Length = 2812
Score = 31.6 bits (70), Expect = 8.8
Identities = 26/91 (28%), Positives = 41/91 (44%), Gaps = 1/91 (1%)
Frame = -3
Query: 296 PLKKPMEAKRKPTIVVKNRTVS-EEPRQSNVALNIAGQKPDIHAGKAIRFWTENWPTNTK 120
P +KP KPTI + T+S EEP I+ +KP I K TE T+ +
Sbjct: 776 PTEKPTIPTEKPTIPTEKPTISTEEPTTPTEETTISTEKPSIPMEKP-TLPTEETTTSVE 834
Query: 119 EAIVAGKRRSDAFSL*KMQMREEREKPTKPS 27
E ++ ++ ++ + EKPT P+
Sbjct: 835 ETTIS----TEKLTIPMEKPTISTEKPTIPT 861
Database: BlastDB/NCBI/blast/db/FASTA/2004_07_04_01_00_0/nr
Posted date: Jul 5, 2004 10:54 PM
Number of letters in database: 629,712,918
Number of sequences in database: 1,895,298
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 629,712,918
effective HSP length: 118
effective length of database: 406,067,754
effective search space used: 25176200748
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)