BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AV548579.r
(627 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAM28874.1|AF503757_1 long chain acyl-CoA synthetase 7 [... 420 e-117
ref|NP_198112.2| long-chain-fatty-acid--CoA ligase-like pro... 420 e-117
pir||T07944 probable long-chain-fatty-acid-CoA ligase (EC 6... 328 3e-89
gb|AAF23219.1|AC013454_6 putative long-chain-fatty-acid--Co... 320 7e-87
dbj|BAB40450.1| long-chain acyl-CoA synthetase [Arabidopsis... 320 7e-87
>gb|AAM28874.1|AF503757_1 long chain acyl-CoA synthetase 7 [Arabidopsis thaliana]
Length = 700
Score = 420 bits (1079), Expect = e-117
Identities = 208/208 (100%), Positives = 208/208 (100%)
Frame = +2
Query: 2 TGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGS 181
TGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGS
Sbjct: 234 TGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGS 293
Query: 182 SVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFC 361
SVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFC
Sbjct: 294 SVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFC 353
Query: 362 SVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEK 541
SVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEK
Sbjct: 354 SVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEK 413
Query: 542 LGGRVRFMGSGASPLSPDVMDFLRICFG 625
LGGRVRFMGSGASPLSPDVMDFLRICFG
Sbjct: 414 LGGRVRFMGSGASPLSPDVMDFLRICFG 441
>ref|NP_198112.2| long-chain-fatty-acid--CoA ligase-like protein; protein id:
At5g27600.1, supported by cDNA: gi_20805874 [Arabidopsis
thaliana]
Length = 700
Score = 420 bits (1079), Expect = e-117
Identities = 208/208 (100%), Positives = 208/208 (100%)
Frame = +2
Query: 2 TGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGS 181
TGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGS
Sbjct: 234 TGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGS 293
Query: 182 SVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFC 361
SVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFC
Sbjct: 294 SVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFC 353
Query: 362 SVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEK 541
SVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEK
Sbjct: 354 SVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEK 413
Query: 542 LGGRVRFMGSGASPLSPDVMDFLRICFG 625
LGGRVRFMGSGASPLSPDVMDFLRICFG
Sbjct: 414 LGGRVRFMGSGASPLSPDVMDFLRICFG 441
>pir||T07944 probable long-chain-fatty-acid-CoA ligase (EC 6.2.1.3) -
rape|gi|1617274|emb|CAA96522.1| AMP-binding protein
[Brassica napus]
Length = 677
Score = 328 bits (841), Expect = 3e-89
Identities = 155/207 (74%), Positives = 182/207 (87%)
Frame = +2
Query: 5 GVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGSS 184
GV +VSY LL+QGRS+ PF PPKP+D+AT+CYTSGTTGTPKGVVLTH NLIANVAGSS
Sbjct: 231 GVKVVSYSVLLNQGRSNPQPFCPPKPDDVATLCYTSGTTGTPKGVVLTHANLIANVAGSS 290
Query: 185 VEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFCS 364
+FF SD+YISYLPLAHIYERANQI+ VY GVAVGFYQGD KL+DD A LRPT+F S
Sbjct: 291 FSVKFFSSDIYISYLPLAHIYERANQILAVYFGVAVGFYQGDNMKLLDDLAALRPTVFSS 350
Query: 365 VPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEKL 544
VPRLYNRIYDGIT+AVK+SG +K+RLF AYN+KKQA++NG++ S WD+LVFNKIK++L
Sbjct: 351 VPRLYNRIYDGITNAVKTSGGLKERLFNAAYNAKKQALLNGKSASPIWDRLVFNKIKDRL 410
Query: 545 GGRVRFMGSGASPLSPDVMDFLRICFG 625
GGRVRFM SGASPLSP+V++FL+ICFG
Sbjct: 411 GGRVRFMTSGASPLSPEVLEFLKICFG 437
>gb|AAF23219.1|AC013454_6 putative long-chain-fatty-acid--CoA ligase [Arabidopsis thaliana]
Length = 657
Score = 320 bits (821), Expect = 7e-87
Identities = 154/208 (74%), Positives = 180/208 (86%)
Frame = +2
Query: 2 TGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGS 181
+GV +VSY LL+QGRS+ F PPKP+D+ATICYTSGTTGTPKGVVLTH NLIANVAGS
Sbjct: 219 SGVKVVSYSVLLNQGRSNPQRFFPPKPDDVATICYTSGTTGTPKGVVLTHANLIANVAGS 278
Query: 182 SVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFC 361
S +FF SDVYISYLPLAHIYERANQI+ VY GVAVGFYQGD KL+DD A LRPT+F
Sbjct: 279 SFSVKFFSSDVYISYLPLAHIYERANQILTVYFGVAVGFYQGDNMKLLDDLAALRPTVFS 338
Query: 362 SVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEK 541
SVPRLYNRIY GI +AVK+SG +K+RLF AYN+KKQA++NG++ S WD+LVFNKIK++
Sbjct: 339 SVPRLYNRIYAGIINAVKTSGGLKERLFNAAYNAKKQALLNGKSASPIWDRLVFNKIKDR 398
Query: 542 LGGRVRFMGSGASPLSPDVMDFLRICFG 625
LGGRVRFM SGASPLSP+VM+FL++CFG
Sbjct: 399 LGGRVRFMTSGASPLSPEVMEFLKVCFG 426
>dbj|BAB40450.1| long-chain acyl-CoA synthetase [Arabidopsis thaliana]
Length = 701
Score = 320 bits (821), Expect = 7e-87
Identities = 154/208 (74%), Positives = 180/208 (86%)
Frame = +2
Query: 2 TGVTIVSYQKLLSQGRSSLHPFSPPKPEDIATICYTSGTTGTPKGVVLTHGNLIANVAGS 181
+GV +VSY LL+QGRS+ F PPKP+D+ATICYTSGTTGTPKGVVLTH NLIANVAGS
Sbjct: 234 SGVKVVSYSVLLNQGRSNPQRFFPPKPDDVATICYTSGTTGTPKGVVLTHANLIANVAGS 293
Query: 182 SVEAEFFPSDVYISYLPLAHIYERANQIMGVYGGVAVGFYQGDVFKLMDDFAVLRPTIFC 361
S +FF SDVYISYLPLAHIYERANQI+ VY GVAVGFYQGD KL+DD A LRPT+F
Sbjct: 294 SFSVKFFSSDVYISYLPLAHIYERANQILTVYFGVAVGFYQGDNMKLLDDLAALRPTVFS 353
Query: 362 SVPRLYNRIYDGITSAVKSSGVVKKRLFEIAYNSKKQAIINGRTPSAFWDKLVFNKIKEK 541
SVPRLYNRIY GI +AVK+SG +K+RLF AYN+KKQA++NG++ S WD+LVFNKIK++
Sbjct: 354 SVPRLYNRIYAGIINAVKTSGGLKERLFNAAYNAKKQALLNGKSASPIWDRLVFNKIKDR 413
Query: 542 LGGRVRFMGSGASPLSPDVMDFLRICFG 625
LGGRVRFM SGASPLSP+VM+FL++CFG
Sbjct: 414 LGGRVRFMTSGASPLSPEVMEFLKVCFG 441
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 117
effective length of database: 272,520,159
effective search space used: 24799334469
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)