BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= 2187.f
(687 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_181002.1| putative gibberellin 2-oxidase; protein id... 280 2e-74
ref|NP_565790.1| Expressed protein; protein id: At2g34550.1... 210 2e-53
ref|NP_174296.1| unknown protein; protein id: At1g30040.1 [... 188 5e-47
emb|CAB41036.1| GA 2-oxidase [Phaseolus coccineus] 146 3e-34
gb|AAN87571.1| gibberellin 2-oxidase 1 [Spinacia oleracea] 130 2e-29
>ref|NP_181002.1| putative gibberellin 2-oxidase; protein id: At2g34555.1
[Arabidopsis thaliana]|gi|25285701|pir||T52577
gibberellin 2beta-dioxygenase (EC 1.14.11.13) 3
[validated] - Arabidopsis
thaliana|gi|4678370|emb|CAB41009.1| GA 2-oxidase
[Arabidopsis thaliana]|gi|20197080|gb|AAM14908.1|
putative gibberellin 2-oxidase [Arabidopsis thaliana]
Length = 335
Score = 280 bits (715), Expect = 2e-74
Identities = 133/134 (99%), Positives = 134/134 (99%)
Frame = -3
Query: 508 MVIVLQPASFDSNLYVNPKCKPRPVLIPVIDLTDSDAKTQIIKACEEFGFFKVINHGVRP 329
MVIVLQPASFDSNLYVNPKCKPRPVLIPVIDLTDSDAKTQI+KACEEFGFFKVINHGVRP
Sbjct: 1 MVIVLQPASFDSNLYVNPKCKPRPVLIPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRP 60
Query: 328 DLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESH 149
DLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESH
Sbjct: 61 DLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESH 120
Query: 148 KTTAIFRHTPAIFR 107
KTTAIFRHTPAIFR
Sbjct: 121 KTTAIFRHTPAIFR 134
>ref|NP_565790.1| Expressed protein; protein id: At2g34550.1, supported by cDNA:
2187. [Arabidopsis thaliana]|gi|20197085|gb|AAC26696.2|
Expressed protein [Arabidopsis
thaliana]|gi|21554287|gb|AAM63362.1| unknown
[Arabidopsis thaliana]
Length = 103
Score = 210 bits (534), Expect = 2e-53
Identities = 103/103 (100%), Positives = 103/103 (100%)
Frame = +2
Query: 95 MVVIPENCRGVPENGGGFVRLKAKISIKENVLKPAKVTIGSNPFSTVTKRVRWTRFVFER 274
MVVIPENCRGVPENGGGFVRLKAKISIKENVLKPAKVTIGSNPFSTVTKRVRWTRFVFER
Sbjct: 1 MVVIPENCRGVPENGGGFVRLKAKISIKENVLKPAKVTIGSNPFSTVTKRVRWTRFVFER 60
Query: 275 VMQSKEVDGFLLQLSQKIGSDPMVDDFEEPKLFTCLDDLGFGI 403
VMQSKEVDGFLLQLSQKIGSDPMVDDFEEPKLFTCLDDLGFGI
Sbjct: 61 VMQSKEVDGFLLQLSQKIGSDPMVDDFEEPKLFTCLDDLGFGI 103
>ref|NP_174296.1| unknown protein; protein id: At1g30040.1 [Arabidopsis
thaliana]|gi|25285702|pir||T52578 gibberellin
2beta-dioxygenase (EC 1.14.11.13) 2 [validated] -
Arabidopsis thaliana|gi|4678368|emb|CAB41008.1| GA
2-oxidase [Arabidopsis
thaliana]|gi|12324163|gb|AAG52050.1|AC022455_4 unknown
protein; 59645-61446 [Arabidopsis
thaliana]|gi|27754705|gb|AAO22796.1| unknown protein
[Arabidopsis thaliana]|gi|28394101|gb|AAO42458.1|
unknown protein [Arabidopsis thaliana]
Length = 341
Score = 188 bits (478), Expect = 5e-47
Identities = 88/138 (63%), Positives = 113/138 (81%), Gaps = 4/138 (2%)
Frame = -3
Query: 508 MVIVLQPASFDSNLYVNPKCKPRPVL----IPVIDLTDSDAKTQIIKACEEFGFFKVINH 341
MV++ QP + D+++ + P KP PVL IPV++L D +AKT+I+KACEEFGFFKV+NH
Sbjct: 1 MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60
Query: 340 GVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLC 161
GVRP+L+T+LEQEAI FF L SLK++AGPP+P+GYG KRIGPNGD+GW+EY+LLNAN
Sbjct: 61 GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120
Query: 160 LESHKTTAIFRHTPAIFR 107
L S KT+A+FR TP IFR
Sbjct: 121 LSSPKTSAVFRQTPQIFR 138
>emb|CAB41036.1| GA 2-oxidase [Phaseolus coccineus]
Length = 332
Score = 146 bits (368), Expect = 3e-34
Identities = 73/134 (54%), Positives = 90/134 (66%)
Frame = -3
Query: 508 MVIVLQPASFDSNLYVNPKCKPRPVLIPVIDLTDSDAKTQIIKACEEFGFFKVINHGVRP 329
MV++ QPA L K P IPV+DLT DAK I+ AC +FGFFK++NHGV
Sbjct: 1 MVVLSQPALNQFFLLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPL 60
Query: 328 DLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESH 149
+L+ LE EA+ FF S KD+AGPPDPFGYG+KRIGPNGD+GW+EY+LLN N + S
Sbjct: 61 ELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISP 120
Query: 148 KTTAIFRHTPAIFR 107
K+ IFR P FR
Sbjct: 121 KSLCIFRENPHHFR 134
>gb|AAN87571.1| gibberellin 2-oxidase 1 [Spinacia oleracea]
Length = 337
Score = 130 bits (327), Expect = 2e-29
Identities = 65/128 (50%), Positives = 86/128 (66%), Gaps = 7/128 (5%)
Frame = -3
Query: 451 CKPR-----PVLIPVIDLTDSDAKTQIIKACEEFGFFKVINHGVRPDLLTQLEQEAINFF 287
CKP P +IP IDL D K+ I+KAC+EFGFFKV+NHGV ++ QLE EA FF
Sbjct: 16 CKPIDTHLFPTVIPTIDLNSPDVKSLIVKACKEFGFFKVVNHGVPIEMTRQLEDEASKFF 75
Query: 286 ALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFR 107
+L LK+ AG P+PFGYG K+IG NGD+GW+EY+LL+A+ S +I+ P +FR
Sbjct: 76 SLPQGLKESAGNPNPFGYGNKKIGRNGDVGWIEYLLLSASDEFISQTCLSIYPDNPDVFR 135
Query: 106 --YNNHIN 89
NN+I+
Sbjct: 136 CALNNYIS 143
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 119
effective length of database: 269,842,067
effective search space used: 29412785303
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)