BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= At1g73420.f
(270 letters)
Database: BlastDB/NCBI/blast/db/2003_02_27_02_00_4/nr
1,347,713 sequences; 431,508,457 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_009044.1| trichohyalin [Homo sapiens]|gi|292836|gb|A... 35 0.20
emb|CAD25183.1| hypothetical protein [Encephalitozoon cunic... 33 0.76
dbj|BAA01446.1| nuclear protein [Saccharomyces cerevisiae]|... 33 0.76
sp|P53935|YNJ1_YEAST HYPOTHETICAL 141.5 KD PROTEIN IN YPT53... 33 0.76
gb|AAA35120.1| STH1 protein 33 0.76
>ref|NP_009044.1| trichohyalin [Homo sapiens]|gi|292836|gb|AAA65582.1|
trichohyalin|gi|586120|sp|Q07283|TRHY_HUMAN
Trichohyalin|gi|539701|pir||A45973 trichohyalin - human
Length = 1898
Score = 35.4 bits (80), Expect = 0.20
Identities = 16/49 (32%), Positives = 31/49 (62%)
Frame = +1
Query: 55 REHMQRKKFQIPGEMDEKQRRIEIRTIPKTMRNSTEIKREQVKEKRELQ 201
RE +R++ Q+ E +E++R ++R + R +++REQ +E+RE Q
Sbjct: 341 REQEERREQQLRREQEEERREQQLRREQEEERREQQLRREQEEERREQQ 389
Score = 30.4 bits (67), Expect = 6.4
Identities = 17/58 (29%), Positives = 33/58 (56%)
Frame = +1
Query: 31 KELSRKKPREHMQRKKFQIPGEMDEKQRRIEIRTIPKTMRNSTEIKREQVKEKRELQQ 204
+E RK R+ ++R++ +E+Q++ +R + R E +REQ +E+RE Q+
Sbjct: 275 EEQLRKLERQELRRER------QEEEQQQQRLRREQQLRRKQEEERREQQEERREQQE 326
>emb|CAD25183.1| hypothetical protein [Encephalitozoon
cuniculi]|gi|19074073|ref|NP_584679.1| hypothetical
protein [Encephalitozoon cuniculi]
Length = 616
Score = 33.5 bits (75), Expect = 0.76
Identities = 21/63 (33%), Positives = 33/63 (52%)
Frame = +1
Query: 19 QGMSKELSRKKPREHMQRKKFQIPGEMDEKQRRIEIRTIPKTMRNSTEIKREQVKEKREL 198
+G+ E RE +RK+ + EM+E++RR E R +N E+ R +EKRE
Sbjct: 302 KGIDDESINNAVREVRERKRLEEMREMEERKRREEERA-----KNEEELLRMVEREKREE 356
Query: 199 QQG 207
+G
Sbjct: 357 SKG 359
>dbj|BAA01446.1| nuclear protein [Saccharomyces cerevisiae]|gi|1078159|pir||S49883
nuclear protein STH1 - yeast (Saccharomyces
cerevisiae)|gi|599991|emb|CAA86866.1| nuclear protein
[Saccharomyces cerevisiae]|gi|6322065|ref|NP_012140.1|
helicase related protein, snf2 homolog; Sth1p
[Saccharomyces
cerevisiae]|gi|417373|sp|P32597|STH1_YEAST NUCLEAR
PROTEIN STH1/NPS1
Length = 1359
Score = 33.5 bits (75), Expect = 0.76
Identities = 14/38 (36%), Positives = 29/38 (75%), Gaps = 3/38 (7%)
Frame = +1
Query: 109 QRRIEIRT---IPKTMRNSTEIKREQVKEKRELQQGMH 213
QR ++IR+ +P+T+R + E++R+Q+ EKR+ ++ +H
Sbjct: 286 QRSVQIRSKVIVPQTVRLAEELERQQLLEKRKKERNLH 323
>sp|P53935|YNJ1_YEAST HYPOTHETICAL 141.5 KD PROTEIN IN YPT53-RHO2 INTERGENIC
REGION|gi|758297|emb|CAA59826.1| ORF N2231
[Saccharomyces cerevisiae]|gi|1301994|emb|CAA95967.1|
ORF YNL091w [Saccharomyces
cerevisiae]|gi|6324238|ref|NP_014308.1| Negative
affector of Salt Tolerance; Nst1p
[Saccharomyces cerevisiae]|gi|1077462|pir||S52734
hypothetical protein YNL091w - yeast (Saccharomyces
cerevisiae)
Length = 1240
Score = 33.5 bits (75), Expect = 0.76
Identities = 17/58 (29%), Positives = 35/58 (60%)
Frame = +1
Query: 31 KELSRKKPREHMQRKKFQIPGEMDEKQRRIEIRTIPKTMRNSTEIKREQVKEKRELQQ 204
+E RKK E ++K + ++E+QRR E++ K + E+KR++ +EK+ +++
Sbjct: 689 REAQRKKVEEAKRKKDEERKRRLEEQQRREEMQ--EKQRKQKEELKRKREEEKKRIRE 744
>gb|AAA35120.1| STH1 protein
Length = 1352
Score = 33.5 bits (75), Expect = 0.76
Identities = 14/38 (36%), Positives = 29/38 (75%), Gaps = 3/38 (7%)
Frame = +1
Query: 109 QRRIEIRT---IPKTMRNSTEIKREQVKEKRELQQGMH 213
QR ++IR+ +P+T+R + E++R+Q+ EKR+ ++ +H
Sbjct: 286 QRSVQIRSKVIVPQTVRLAEELERQQLLEKRKKERNLH 323
Database: BlastDB/NCBI/blast/db/2003_02_27_02_00_4/nr
Posted date: Feb 27, 2003 4:02 PM
Number of letters in database: 431,508,457
Number of sequences in database: 1,347,713
Lambda K H
0.315 0.129 0.345
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 431,508,457
effective HSP length: 65
effective length of database: 343,907,112
effective search space used: 8253770688
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)