BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= At1g01980.f
(1626 letters)
Database: BlastDB/NCBI/blast/db/2003_02_27_02_00_4/nr
1,347,713 sequences; 431,508,457 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||G86151 F22M8.11 protein - Arabidopsis thaliana|gi|8570... 995 0.0
gb|AAD30246.1|AC007296_7 Strong similarity to gb|AF049347 b... 808 0.0
gb|AAL32673.1| Unknown protein [Arabidopsis thaliana]|gi|15... 656 0.0
emb|CAB45850.1| reticuline oxidase-like protein [Arabidopsi... 653 0.0
pir||A86433 T5I8.19 protein - Arabidopsis thaliana|gi|45875... 630 e-179
>pir||G86151 F22M8.11 protein - Arabidopsis
thaliana|gi|8570449|gb|AAF76476.1|AC020622_10 Contains
similarity to berberine bridge enzyme from Berberis
stolonifera gb|AF049347 and contains a FAD binding
PF|01565 domain. [Arabidopsis
thaliana]|gi|15217586|ref|NP_171700.1| FAD-linked
oxidoreductase family; protein id: At1g01980.1
[Arabidopsis thaliana]
Length = 541
Score = 995 bits (2572), Expect = 0.0
Identities = 493/522 (94%), Positives = 493/522 (94%)
Frame = +1
Query: 58 ATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAYIRNARFNTSTSPK 237
ATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAYIRNARFNTSTSPK
Sbjct: 20 ATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAYIRNARFNTSTSPK 79
Query: 238 PLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPFFVLDMSYLRNITV 417
PLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPFFVLDMSYLRNITV
Sbjct: 80 PLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPFFVLDMSYLRNITV 139
Query: 418 DMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVXXXXXXXXXXXXNMIR 597
DMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTV NMIR
Sbjct: 140 DMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVGAGGHISGGGYGNMIR 199
Query: 598 KYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIXXXXXASFGVILSFKIKLXXXXXXX 777
KYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAI ASFGVILSFKIKL
Sbjct: 200 KYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIGGGGGASFGVILSFKIKLVPVPPRV 259
Query: 778 XXXXXEKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVTRNTTQTVRASVVALFLGKQ 957
EKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVTRNTTQTVRASVVALFLGKQ
Sbjct: 260 TVFRVEKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVTRNTTQTVRASVVALFLGKQ 319
Query: 958 SDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKPEILLDRNPDSASFLKR 1137
SDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKPEILLDRNPDSASFLKR
Sbjct: 320 SDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKPEILLDRNPDSASFLKR 379
Query: 1138 KSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATTATPFPHRKRLFKVQHS 1317
KSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATTATPFPHRKRLFKVQHS
Sbjct: 380 KSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATTATPFPHRKRLFKVQHS 439
Query: 1318 MNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDLDIGINSHGPNSYREAEV 1497
MNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDLDIGINSHGPNSYREAEV
Sbjct: 440 MNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDLDIGINSHGPNSYREAEV 499
Query: 1498 YGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTLPTKPSSS 1623
YGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTLPTKPSSS
Sbjct: 500 YGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTLPTKPSSS 541
>gb|AAD30246.1|AC007296_7 Strong similarity to gb|AF049347 berberine bridge enzyme from
Berberis stolonifera. [Arabidopsis
thaliana]|gi|25345666|pir||F86251 hypothetical protein
[imported] - Arabidopsis
thaliana|gi|15221093|ref|NP_172642.1| FAD-linked
oxidoreductase family; protein id: At1g11770.1
[Arabidopsis thaliana]
Length = 536
Score = 808 bits (2086), Expect = 0.0
Identities = 394/520 (75%), Positives = 442/520 (84%)
Frame = +1
Query: 58 ATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAYIRNARFNTSTSPK 237
A PP +IY++FLQCF+NQT APP L DVVLP+++A+FTP LRAYIRNARFNT+ +PK
Sbjct: 20 AVEPPPETIYQNFLQCFTNQTKAPPNSLADVVLPKTAAAFTPVLRAYIRNARFNTTATPK 79
Query: 238 PLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPFFVLDMSYLRNITV 417
P +VIAARSE HVQA V+CTKSLN QLKTRSGGHDY+GVSYIS+ PFFVLDMS LRNITV
Sbjct: 80 PAIVIAARSESHVQAAVICTKSLNIQLKTRSGGHDYEGVSYISHVPFFVLDMSNLRNITV 139
Query: 418 DMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVXXXXXXXXXXXXNMIR 597
D + + SAWVGAGATLGEVYY IW+ +K+HG FPAGVCPTV NMIR
Sbjct: 140 DPATE--SAWVGAGATLGEVYYRIWEKTKSHG---FPAGVCPTVGAGGHISGGGYGNMIR 194
Query: 598 KYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIXXXXXASFGVILSFKIKLXXXXXXX 777
KYGLSVDYVTDAKIVDVNG++LDRK MGED+FWAI ASFGVIL+FKIKL
Sbjct: 195 KYGLSVDYVTDAKIVDVNGQVLDRKGMGEDMFWAINGGGGASFGVILAFKIKLVPVPPTV 254
Query: 778 XXXXXEKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVTRNTTQTVRASVVALFLGKQ 957
EK LVENA +MVHKWQFVAPKT P LFMRL+LQPVTRN QTVRASVVALFLG Q
Sbjct: 255 TVFRVEKNLVENATEMVHKWQFVAPKTDPGLFMRLLLQPVTRNKMQTVRASVVALFLGDQ 314
Query: 958 SDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKPEILLDRNPDSASFLKR 1137
+ +MS+LTKEFPELGLK ENCTEMTWIQSVMWWANNDNAT IKPEILLDRNPD A+F KR
Sbjct: 315 NTVMSMLTKEFPELGLKKENCTEMTWIQSVMWWANNDNATQIKPEILLDRNPDMATFGKR 374
Query: 1138 KSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATTATPFPHRKRLFKVQHS 1317
KSD+VEKEI+KDGLDFL KK++E GK+GLVFNPYGG MS VATT TPFPHRK+L+K+QHS
Sbjct: 375 KSDFVEKEITKDGLDFLFKKMIEVGKIGLVFNPYGGIMSTVATTKTPFPHRKKLYKIQHS 434
Query: 1318 MNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDLDIGINSHGPNSYREAEV 1497
MNWKDPGT+ E+SF++K +SFYSYMAPFVTKNPRHTY+NYRDLDIG+N+ GPNSYR AEV
Sbjct: 435 MNWKDPGTEAETSFLQKAKSFYSYMAPFVTKNPRHTYINYRDLDIGVNTPGPNSYRVAEV 494
Query: 1498 YGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTLPTKPS 1617
+GR YFGENFDRLVKVKTAVDP+NFFRDEQSIPTLP KP+
Sbjct: 495 FGRMYFGENFDRLVKVKTAVDPQNFFRDEQSIPTLPGKPA 534
>gb|AAL32673.1| Unknown protein [Arabidopsis
thaliana]|gi|15983493|gb|AAL11614.1|AF424621_1
AT4g20830/F21C20_180 [Arabidopsis
thaliana]|gi|7488295|pir||T10625 reticuline oxidase
homolog F21C20.180 - Arabidopsis
thaliana|gi|5262223|emb|CAB45849.1| reticuline
oxidase-like protein [Arabidopsis
thaliana]|gi|7268879|emb|CAB79083.1| reticuline
oxidase-like protein [Arabidopsis
thaliana]|gi|22137036|gb|AAM91363.1| At4g20830/F21C20_180
[Arabidopsis thaliana]|gi|15233414|ref|NP_193815.1|
FAD-linked oxidoreductase family; protein id:
At4g20830.1, supported by cDNA: gi_15983492 [Arabidopsis
thaliana]
Length = 540
Score = 656 bits (1693), Expect = 0.0
Identities = 308/513 (60%), Positives = 395/513 (76%)
Frame = +1
Query: 73 NTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAYIRNARFNTSTSPKPLLVI 252
+ S+Y SFL+CFS++T +P ++ D V Q++ +F+ LRAYIRNARFNTS++ KP ++I
Sbjct: 32 SNSVYNSFLKCFSDKTKSPQSQITDNVFSQTNPAFSSVLRAYIRNARFNTSSTLKPTIII 91
Query: 253 AARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPFFVLDMSYLRNITVDMSDD 432
RSE HV A V C+K+LNF LK RSGGHDYDG+SYIS++PFF+LDMS +R+++VD++ +
Sbjct: 92 TPRSESHVSAAVTCSKTLNFLLKIRSGGHDYDGLSYISDKPFFILDMSNIRDVSVDIASN 151
Query: 433 GGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVXXXXXXXXXXXXNMIRKYGLS 612
SAW+ AGATLGEVYY IW+ S+ HG FPAGVCPTV NM+RK+GLS
Sbjct: 152 --SAWISAGATLGEVYYRIWEKSRVHG---FPAGVCPTVGVGGHLSGGGYGNMVRKFGLS 206
Query: 613 VDYVTDAKIVDVNGRILDRKSMGEDLFWAIXXXXXASFGVILSFKIKLXXXXXXXXXXXX 792
VDYV DAKIVDVNGR+LDRK+MGEDLFWAI S+GV+L +K+KL
Sbjct: 207 VDYVEDAKIVDVNGRVLDRKAMGEDLFWAITGGGGGSYGVVLGYKVKLVPVPSVVTVFRV 266
Query: 793 EKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVTRNTTQTVRASVVALFLGKQSDLMS 972
E+ + A+DMVHKWQ V PKT P+LFMR+++QPVTR +TVRASVVALFLG+ ++++
Sbjct: 267 EQYMDSGAVDMVHKWQSVGPKTDPNLFMRMLIQPVTRKKVKTVRASVVALFLGRADEVVA 326
Query: 973 LLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKPEILLDRNPDSASFLKRKSDYV 1152
LL+KEFPELGLK ENC+EMTW QS +WW N NAT + P++ LDRN D++SF KRKSDYV
Sbjct: 327 LLSKEFPELGLKKENCSEMTWFQSALWWDNRLNATQVDPKVFLDRNLDTSSFGKRKSDYV 386
Query: 1153 EKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATTATPFPHRKRLFKVQHSMNWKD 1332
I K G++ L KK++E GK+GLVFNPYGGKM+EVA A PFPHR +LFK+Q+S+NWK+
Sbjct: 387 ATAIPKKGIESLFKKMIELGKIGLVFNPYGGKMAEVAVNAKPFPHRNKLFKIQYSVNWKE 446
Query: 1333 PGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDLDIGINSHGPNSYREAEVYGRKY 1512
++E ++ + + YS+M FV+KNPR +Y NYRD+DIG+N HG NSY+E EVYGRKY
Sbjct: 447 NSAEIEKGYLNQAKVLYSFMTGFVSKNPRSSYFNYRDVDIGVNDHGANSYKEGEVYGRKY 506
Query: 1513 FGENFDRLVKVKTAVDPENFFRDEQSIPTLPTK 1611
FGENFDRLVK+KTAVDP NFFR+EQSIPTL +K
Sbjct: 507 FGENFDRLVKIKTAVDPGNFFRNEQSIPTLKSK 539
>emb|CAB45850.1| reticuline oxidase-like protein [Arabidopsis
thaliana]|gi|7488296|pir||T10626 reticuline oxidase
homolog F21C20.190 - Arabidopsis
thaliana|gi|15233416|ref|NP_193816.1| FAD-linked
oxidoreductase family; protein id: At4g20840.1
[Arabidopsis thaliana]|gi|7268880|emb|CAB79084.1|
reticuline oxidase-like protein [Arabidopsis thaliana]
Length = 539
Score = 653 bits (1684), Expect = 0.0
Identities = 310/518 (59%), Positives = 392/518 (74%)
Frame = +1
Query: 58 ATAPPNTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAYIRNARFNTSTSPK 237
A P + SIYESF+QCFS++T +P ++ D V +++ SF+ LRAYIRN RFNTS++PK
Sbjct: 26 AAPPSSDSIYESFVQCFSDKTKSPQAQITDNVFSRTNPSFSSVLRAYIRNGRFNTSSTPK 85
Query: 238 PLLVIAARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPFFVLDMSYLRNITV 417
P +++ RS+ HV A V C+KSLNF LK RSGGHDY+G+SYIS++PFF+LDMS LR+++V
Sbjct: 86 PAIIVTPRSDIHVSAAVTCSKSLNFLLKIRSGGHDYEGLSYISDKPFFILDMSNLRDVSV 145
Query: 418 DMSDDGGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVXXXXXXXXXXXXNMIR 597
D++D SAW+ AGATLGEVYY IW+ SK HG FPAGVCPTV NM+R
Sbjct: 146 DIADQ--SAWISAGATLGEVYYRIWEKSKVHG---FPAGVCPTVGVGGHISGGGYGNMLR 200
Query: 598 KYGLSVDYVTDAKIVDVNGRILDRKSMGEDLFWAIXXXXXASFGVILSFKIKLXXXXXXX 777
K+GLSVD + DAKIVDVNG+ILDRKSMGEDLFWAI ASFGV+L +K+KL
Sbjct: 201 KFGLSVDNLIDAKIVDVNGQILDRKSMGEDLFWAISGGGGASFGVVLGYKVKLVPVPETV 260
Query: 778 XXXXXEKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVTRNTTQTVRASVVALFLGKQ 957
EK + A+DMVHKWQ V PKT +LF+R+++QPVTR +TVRA+VVALFLG+
Sbjct: 261 TVFRVEKYMDSGAVDMVHKWQSVGPKTDRNLFLRMLIQPVTRKKVKTVRATVVALFLGRA 320
Query: 958 SDLMSLLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKPEILLDRNPDSASFLKR 1137
++++LL KEFPEL LK ENC+EMTW QS +WW N N T I P++ LDRN D A+F KR
Sbjct: 321 EEVVALLGKEFPELSLKKENCSEMTWFQSALWWDNRVNPTQIDPKVFLDRNLDRANFGKR 380
Query: 1138 KSDYVEKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATTATPFPHRKRLFKVQHS 1317
KSDYV EI +DG++ L KK+ E GK+GLVFNPYGGKM+EV ATPFPHR +LFK+Q+S
Sbjct: 381 KSDYVASEIPRDGIESLFKKMTELGKIGLVFNPYGGKMAEVTVNATPFPHRSKLFKIQYS 440
Query: 1318 MNWKDPGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDLDIGINSHGPNSYREAEV 1497
+ W++ ++E F+ + YS+M FV+KNPR+ YLNYRD+DIG+N HG NSY E EV
Sbjct: 441 VTWQENSVEIEKGFLNQANVLYSFMTGFVSKNPRNAYLNYRDVDIGVNDHGTNSYEEGEV 500
Query: 1498 YGRKYFGENFDRLVKVKTAVDPENFFRDEQSIPTLPTK 1611
YGRKYFG+NFDRLVKVKTA DP+NFFR+EQSIPT+ +K
Sbjct: 501 YGRKYFGDNFDRLVKVKTAADPDNFFRNEQSIPTVLSK 538
>pir||A86433 T5I8.19 protein - Arabidopsis
thaliana|gi|4587530|gb|AAD25761.1|AC007060_19 Strong
similarity to F19I3.2 gi|3033375 putative berberine
bridge enzyme from Arabidopsis thaliana BAC
gb|AC004238|gi|15221497|ref|NP_174361.1| FAD-linked
oxidoreductase family; protein id: At1g30740.1
[Arabidopsis thaliana]
Length = 533
Score = 630 bits (1625), Expect = e-179
Identities = 302/511 (59%), Positives = 377/511 (73%)
Frame = +1
Query: 73 NTSIYESFLQCFSNQTGAPPEKLCDVVLPQSSASFTPTLRAYIRNARFNTSTSPKPLLVI 252
+ +IYE F+QCF N T L DVVLP++S SFTPTLRAYIRNARFNTS+ PKP ++I
Sbjct: 22 SVTIYEDFVQCFKNVTTISDIDLSDVVLPRTSISFTPTLRAYIRNARFNTSSMPKPSIII 81
Query: 253 AARSECHVQATVLCTKSLNFQLKTRSGGHDYDGVSYISNRPFFVLDMSYLRNITVDMSDD 432
R + HVQA V+C K+LN QLK RSGGHDYDG+SY+S F VLD+S RNITVD++D
Sbjct: 82 VPRVDSHVQAAVICAKTLNLQLKIRSGGHDYDGLSYVSAVTFLVLDLSNFRNITVDLNDG 141
Query: 433 GGSAWVGAGATLGEVYYNIWQSSKTHGTHGFPAGVCPTVXXXXXXXXXXXXNMIRKYGLS 612
GGSAWV GATLGE+YY IW+ S+ H FPAGVCPTV +MIRK+GL+
Sbjct: 142 GGSAWVQTGATLGELYYRIWEKSEVHA---FPAGVCPTVGVGGHVSGGGYGHMIRKFGLT 198
Query: 613 VDYVTDAKIVDVNGRILDRKSMGEDLFWAIXXXXXASFGVILSFKIKLXXXXXXXXXXXX 792
+D+V DA IVD NG+I DRKSM EDLFWAI SFGV+L+FK+KL
Sbjct: 199 IDHVVDATIVDANGQIHDRKSMEEDLFWAIRGGGGGSFGVVLAFKVKLVTVPKTVTVFRV 258
Query: 793 EKTLVENALDMVHKWQFVAPKTSPDLFMRLMLQPVTRNTTQTVRASVVALFLGKQSDLMS 972
+K++ ENALDMV+KWQFVAP+T P LFMR++L T+N T TV + AL+LGK D++
Sbjct: 259 DKSVDENALDMVYKWQFVAPRTDPGLFMRVLLSSPTQNKTSTVNTKLRALYLGKADDVVL 318
Query: 973 LLTKEFPELGLKPENCTEMTWIQSVMWWANNDNATVIKPEILLDRNPDSASFLKRKSDYV 1152
+ +EFPELGLK E+C EMTWIQS++WW N+ + +KPEILL+R PDSA FLKRKSDYV
Sbjct: 319 KMAEEFPELGLKKEDCKEMTWIQSLLWWMNHVDVDKVKPEILLEREPDSAKFLKRKSDYV 378
Query: 1153 EKEISKDGLDFLCKKLMEAGKLGLVFNPYGGKMSEVATTATPFPHRKRLFKVQHSMNWKD 1332
EKE++K L+ L +KL + GLV NPYGG ++ A AT FPHR +L+K+QHS+ W D
Sbjct: 379 EKEMTKPELNRLFQKLATLDRTGLVLNPYGGSLNVTAVNATAFPHRHKLYKIQHSVTWPD 438
Query: 1333 PGTDVESSFMEKTRSFYSYMAPFVTKNPRHTYLNYRDLDIGINSHGPNSYREAEVYGRKY 1512
G + E ++ R+ Y+ M PFV+KNPR +YLNYRD+DIG+N HG + YR+ E+YGRKY
Sbjct: 439 AGPEAERLYIGNLRTTYNIMTPFVSKNPRSSYLNYRDIDIGVNDHGADGYRKGEIYGRKY 498
Query: 1513 FGENFDRLVKVKTAVDPENFFRDEQSIPTLP 1605
FGENFDRLV+VKTAVDP+NFFR+EQSIPTLP
Sbjct: 499 FGENFDRLVRVKTAVDPDNFFRNEQSIPTLP 529
Database: BlastDB/NCBI/blast/db/2003_02_27_02_00_4/nr
Posted date: Feb 27, 2003 4:02 PM
Number of letters in database: 431,508,457
Number of sequences in database: 1,347,713
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 431,508,457
effective HSP length: 128
effective length of database: 259,001,193
effective search space used: 106967492709
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)