BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= At1g01460.f
(1278 letters)
Database: BlastDB/NCBI/blast/db/2003_02_27_02_00_4/nr
1,347,713 sequences; 431,508,457 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_171653.1| 4,5 PIP kinase, putative; protein id: At1g... 816 0.0
emb|CAA73312.1| 4,5 PIP kinase [Arabidopsis thaliana] 811 0.0
pir||C86145 hypothetical protein F22L4.2 - Arabidopsis thal... 769 0.0
pir||F85015 probable phosphatidylinositol kinase [imported]... 536 e-151
pir||T01723 1-phosphatidylinositol-4-phosphate 5-kinase typ... 516 e-145
>ref|NP_171653.1| 4,5 PIP kinase, putative; protein id: At1g01460.1 [Arabidopsis
thaliana]
Length = 425
Score = 816 bits (2108), Expect = 0.0
Identities = 409/425 (96%), Positives = 409/425 (96%)
Frame = +1
Query: 1 MFTREITAKDVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDE 180
MFTREITAKDVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDE
Sbjct: 1 MFTREITAKDVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDE 60
Query: 181 YMKSICNDETLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRS 360
YMKSICNDETLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRS
Sbjct: 61 YMKSICNDETLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRS 120
Query: 361 TLLSKNYGAHSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTI 540
TLLSKNYGAHSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTI
Sbjct: 121 TLLSKNYGAHSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTI 180
Query: 541 LKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCHGSIDELIP 720
LKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCHGSIDELIP
Sbjct: 181 LKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCHGSIDELIP 240
Query: 721 VYDSFTSRGSVDSNSSKFMKTASNSPDRSSSTMYSCTPSRIDSENSVNIQSVASISPSPA 900
VYDSFTSRGSVDSNSSKFMKTASNSPDRSSSTMYSCTPSRIDSENSVNIQSVASISPSPA
Sbjct: 241 VYDSFTSRGSVDSNSSKFMKTASNSPDRSSSTMYSCTPSRIDSENSVNIQSVASISPSPA 300
Query: 901 QTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIPXXXXXXXXXXXXXXXXKQSREGGEE 1080
QTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIP KQSREGGEE
Sbjct: 301 QTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIPGRARRVGRGESGSVVGKQSREGGEE 360
Query: 1081 WYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFLPD 1260
WYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFLPD
Sbjct: 361 WYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFLPD 420
Query: 1261 EDPSH 1275
EDPSH
Sbjct: 421 EDPSH 425
>emb|CAA73312.1| 4,5 PIP kinase [Arabidopsis thaliana]
Length = 427
Score = 811 bits (2094), Expect = 0.0
Identities = 408/427 (95%), Positives = 409/427 (95%), Gaps = 2/427 (0%)
Frame = +1
Query: 1 MFTREITAKDVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDE 180
MFTREITAKDVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDE
Sbjct: 1 MFTREITAKDVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDE 60
Query: 181 YMKSICNDETLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRS 360
YMKSICNDETLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRS
Sbjct: 61 YMKSICNDETLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRS 120
Query: 361 TLLSKNYGAHSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTI 540
TLLSKNYGAHSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTI
Sbjct: 121 TLLSKNYGAHSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTI 180
Query: 541 LKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCHGSIDELIP 720
LKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCHGSIDELIP
Sbjct: 181 LKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCHGSIDELIP 240
Query: 721 VYDSFTSRGSVDSNSSKFMKTASNSPDRSSSTMYSCTPSR--IDSENSVNIQSVASISPS 894
VYDSFTSRGSVDSNSSKFMKTASNSPDRSSSTMYSCTPSR +DSENSVNIQSVASISPS
Sbjct: 241 VYDSFTSRGSVDSNSSKFMKTASNSPDRSSSTMYSCTPSRNSVDSENSVNIQSVASISPS 300
Query: 895 PAQTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIPXXXXXXXXXXXXXXXXKQSREGG 1074
PAQTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIP KQSREGG
Sbjct: 301 PAQTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIPGRARRVGRGESGSVVGKQSREGG 360
Query: 1075 EEWYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFL 1254
EEWYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFL
Sbjct: 361 EEWYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFL 420
Query: 1255 PDEDPSH 1275
PDEDPSH
Sbjct: 421 PDEDPSH 427
>pir||C86145 hypothetical protein F22L4.2 - Arabidopsis
thaliana|gi|8920584|gb|AAF81306.1|AC061957_2 Identical to
a 4,5 PIP kinase from Arabidopsis thaliana gb|Y12776 and
contains a Phosphatidylinositol-4-phosphate 5-kinase
PF|01504 domain
Length = 411
Score = 769 bits (1986), Expect = 0.0
Identities = 392/427 (91%), Positives = 393/427 (91%), Gaps = 2/427 (0%)
Frame = +1
Query: 1 MFTREITAKDVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDE 180
MFTREITAKDVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDE
Sbjct: 1 MFTREITAKDVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDE 60
Query: 181 YMKSICNDETLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRS 360
YMKSICNDETLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRS
Sbjct: 61 YMKSICNDETLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRS 120
Query: 361 TLLSKNYGAHSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTI 540
TLLSKNYGAHSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTI
Sbjct: 121 TLLSKNYGAHSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTI 180
Query: 541 LKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCHGSIDELIP 720
LKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCH
Sbjct: 181 LKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCH-------- 232
Query: 721 VYDSFTSRGSVDSNSSKFMKTASNSPDRSSSTMYSCTPSR--IDSENSVNIQSVASISPS 894
GSVDSNSSKFMKTASNSPDRSSSTMYSCTPSR +DSENSVNIQSVASISPS
Sbjct: 233 --------GSVDSNSSKFMKTASNSPDRSSSTMYSCTPSRNSVDSENSVNIQSVASISPS 284
Query: 895 PAQTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIPXXXXXXXXXXXXXXXXKQSREGG 1074
PAQTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIP KQSREGG
Sbjct: 285 PAQTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIPGRARRVGRGESGSVVGKQSREGG 344
Query: 1075 EEWYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFL 1254
EEWYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFL
Sbjct: 345 EEWYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFL 404
Query: 1255 PDEDPSH 1275
PDEDPSH
Sbjct: 405 PDEDPSH 411
>pir||F85015 probable phosphatidylinositol kinase [imported] - Arabidopsis
thaliana|gi|7267616|emb|CAB80928.1| putative
phosphatidylinositol kinase [Arabidopsis
thaliana]|gi|15234094|ref|NP_192028.1| putative
phosphatidylinositol kinase; protein id: At4g01190.1
[Arabidopsis thaliana]
Length = 401
Score = 536 bits (1381), Expect = e-151
Identities = 266/416 (63%), Positives = 334/416 (79%), Gaps = 3/416 (0%)
Frame = +1
Query: 28 DVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDEYMKSICNDE 207
+++AT +NRIRYS+KHIKHLPPG+ITEF+WKDYCP+GF LIQELE I+HD+Y+ SIC DE
Sbjct: 2 ELRATVENRIRYSTKHIKHLPPGSITEFDWKDYCPVGFGLIQELEGIDHDDYLLSICTDE 61
Query: 208 TLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIKVILEMLPGYFRHIHKYRSTLLSKNYGA 387
TL+K+S+ K+GN+F +S D+RFLIKILRKSEIKV LEMLP Y+RHI+ +RS+L ++ +GA
Sbjct: 62 TLKKISSGKIGNVFHISNDNRFLIKILRKSEIKVTLEMLPRYYRHINYHRSSLFTRIFGA 121
Query: 388 HSVKPIGGVKTYFVVMSNILQSDVFMNKVYDLKGSSQGRTNKKIKVRDKTILKDIDLDFC 567
HSVKP+GGVKTYF VMSN+L S +F+NK+YDLKGS +GR+NKKI+VR+ T+LKDID DFC
Sbjct: 122 HSVKPLGGVKTYFAVMSNMLHSTIFVNKLYDLKGSPKGRSNKKIEVRNTTVLKDIDFDFC 181
Query: 568 FYVDSLARHRLIKQTKLDCELLEDEGIMDYSLMLGLQVKGSCHGSIDELIPVYDSFTSRG 747
FYVD LAR R+IKQTKLDCELLE+EGIMDYSL++GLQ KGSC GS+D L PVY SF
Sbjct: 182 FYVDPLARQRIIKQTKLDCELLEEEGIMDYSLLVGLQSKGSCQGSLDGLNPVYGSFAPPS 241
Query: 748 SVDSNSSKFMKTASNSPDRSSSTMYSCTPSR--IDSENSVNIQSVASISPSPAQTNASDS 921
S SNS+K MKTAS+SPDRSS MYSC+P R +++E S+ IQSV S S S ++TN
Sbjct: 242 SFKSNSTKSMKTASSSPDRSSVAMYSCSPDRDSVENEMSMTIQSVTSNSAS-SETN---- 296
Query: 922 PYESLVSKTNLTNIFQNSSSTNFGMKIPXXXXXXXXXXXXXXXXKQSRE-GGEEWYDVIL 1098
+ T L+++F NSS+ NFGMKIP + +RE G EEWY+V+L
Sbjct: 297 -----ILATTLSDLFHNSSNINFGMKIP------------ARARRVTRETGEEEWYNVVL 339
Query: 1099 YLGIIDIFQDYGVRKRLEHCYKSIQHSSKTISAVHPKMYSSRFQDFVSQIFLPDED 1266
Y+GI+D FQDYG++KR+EHCYKSIQ++S +IS VHPK+YSSRFQDFVS IFLP +D
Sbjct: 340 YIGIVDTFQDYGMKKRIEHCYKSIQYNSNSISTVHPKIYSSRFQDFVSNIFLPHDD 395
>pir||T01723 1-phosphatidylinositol-4-phosphate 5-kinase type II homolog -
Arabidopsis thaliana|gi|2191143|gb|AAB61030.1| similar to
phosphatidylinositol-4-phosphate -kinase type II
[Arabidopsis thaliana]
Length = 431
Score = 516 bits (1329), Expect = e-145
Identities = 266/446 (59%), Positives = 334/446 (74%), Gaps = 33/446 (7%)
Frame = +1
Query: 28 DVKATEKNRIRYSSKHIKHLPPGTITEFEWKDYCPLGFRLIQELEDINHDEYMKSICNDE 207
+++AT +NRIRYS+KHIKHLPPG+ITEF+WKDYCP+GF LIQELE I+HD+Y+ SIC DE
Sbjct: 2 ELRATVENRIRYSTKHIKHLPPGSITEFDWKDYCPVGFGLIQELEGIDHDDYLLSICTDE 61
Query: 208 TLRKLSTSKVGNMFLLSKDDRFLIKILRKSEIK-----------------VILEMLPGYF 336
TL+K+S+ K+GN+F +S D+RFLIKILRKSEIK V LEMLP Y+
Sbjct: 62 TLKKISSGKIGNVFHISNDNRFLIKILRKSEIKCVYMFHIESFIENNLVKVTLEMLPRYY 121
Query: 337 RHIHKYRSTLLSKNYGAHSVKPIGGVK-------------TYFVVMSNILQSDVFMNKVY 477
RHI+ +RS+L ++ +GAHSVKP+GGVK TYF VMSN+L S +F+NK+Y
Sbjct: 122 RHINYHRSSLFTRIFGAHSVKPLGGVKVTRIIYIYHSIIRTYFAVMSNMLHSTIFVNKLY 181
Query: 478 DLKGSSQGRTNKKIKVRDKTILKDIDLDFCFYVDSLARHRLIKQTKLDCELLEDEGIMDY 657
DLKGS +GR+NKKI+VR+ T+LKDID DFCFYVD LAR R+IKQTKLDCELLE+EGIMDY
Sbjct: 182 DLKGSPKGRSNKKIEVRNTTVLKDIDFDFCFYVDPLARQRIIKQTKLDCELLEEEGIMDY 241
Query: 658 SLMLGLQVKGSCHGSIDELIPVYDSFTSRGSVDSNSSKFMKTASNSPDRSSSTMYSCTPS 837
SL++GLQ KGSC GS+D L PVY SF S SNS+K MKTAS+SPDRSS MYSC+P
Sbjct: 242 SLLVGLQSKGSCQGSLDGLNPVYGSFAPPSSFKSNSTKSMKTASSSPDRSSVAMYSCSPD 301
Query: 838 R--IDSENSVNIQSVASISPSPAQTNASDSPYESLVSKTNLTNIFQNSSSTNFGMKIPXX 1011
R +++E S+ IQSV S S S ++TN + T L+++F NSS+ NFGMKIP
Sbjct: 302 RDSVENEMSMTIQSVTSNSAS-SETN---------ILATTLSDLFHNSSNINFGMKIP-- 349
Query: 1012 XXXXXXXXXXXXXXKQSRE-GGEEWYDVILYLGIIDIFQDYGVRKRLEHCYKSIQHSSKT 1188
+ +RE G EEWY+V+LY+GI+D FQDYG++KR+EHCYKSIQ++S +
Sbjct: 350 ----------ARARRVTRETGEEEWYNVVLYIGIVDTFQDYGMKKRIEHCYKSIQYNSNS 399
Query: 1189 ISAVHPKMYSSRFQDFVSQIFLPDED 1266
IS VHPK+YSSRFQDFVS IFLP +D
Sbjct: 400 ISTVHPKIYSSRFQDFVSNIFLPHDD 425
Database: BlastDB/NCBI/blast/db/2003_02_27_02_00_4/nr
Posted date: Feb 27, 2003 4:02 PM
Number of letters in database: 431,508,457
Number of sequences in database: 1,347,713
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 431,508,457
effective HSP length: 126
effective length of database: 261,696,619
effective search space used: 78247289081
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)