BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.sd.8117.C1
(1547 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_187819.1| omega-6 fatty acid desaturase, endoplasmic... 798 0.0
gb|AAM61113.1| omega-6 fatty acid desaturase, endoplasmic r... 795 0.0
gb|AAD19742.1| delta-12 desaturase [Brassica carinata] 739 0.0
sp|Q39287|FD6E_BRAJU Omega-6 fatty acid desaturase, endopla... 731 0.0
emb|CAD30827.1| fatty acid desaturase 2 [Brassica rapa] 728 0.0
>ref|NP_187819.1| omega-6 fatty acid desaturase, endoplasmic reticulum (FAD2)(delta-12
desaturase); protein id: At3g12120.1 [Arabidopsis
thaliana]|gi|1169598|sp|P46313|FD6E_ARATH Omega-6 fatty
acid desaturase, endoplasmic reticulum (Delta-12
desaturase)|gi|438451|gb|AAA32782.1| delta-12
desaturase|gi|9294109|dbj|BAB01960.1| omega-6 fatty acid
desaturase, endoplasmic reticulum (delta-12 desaturase)
[Arabidopsis
thaliana]|gi|12322004|gb|AAG51042.1|AC069473_4 omega-6
fatty acid desaturase, endoplasmic reticulum (FAD2);
20389-21540 [Arabidopsis
thaliana]|gi|14517474|gb|AAK62627.1| AT3g12120/T21B14_107
[Arabidopsis thaliana]|gi|22655458|gb|AAM98321.1|
At3g12120/T21B14_107 [Arabidopsis thaliana]
Length = 383
Score = 798 bits (2060), Expect = 0.0
Identities = 371/383 (96%), Positives = 371/383 (96%)
Frame = +1
Query: 88 MGAGGRMPVPXXXXXXXXXXXXRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 267
MGAGGRMPVP RVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI
Sbjct: 1 MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60
Query: 268 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 447
IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD
Sbjct: 61 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120
Query: 448 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 627
TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180
Query: 628 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 807
MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240
Query: 808 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 987
RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300
Query: 988 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 1167
YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK
Sbjct: 301 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360
Query: 1168 ECIYVEPDREGDKKGVYWYNNKL 1236
ECIYVEPDREGDKKGVYWYNNKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383
>gb|AAM61113.1| omega-6 fatty acid desaturase, endoplasmic reticulum (FAD2)
[Arabidopsis thaliana]
Length = 383
Score = 795 bits (2053), Expect = 0.0
Identities = 370/383 (96%), Positives = 370/383 (96%)
Frame = +1
Query: 88 MGAGGRMPVPXXXXXXXXXXXXRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 267
MGAGGRMPVP RVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI
Sbjct: 1 MGAGGRMPVPTSSKKSETDTTKRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 60
Query: 268 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 447
IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD
Sbjct: 61 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 120
Query: 448 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 627
TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 180
Query: 628 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 807
MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY
Sbjct: 181 MLTVQFVLGWPLYLAFNVSGRPYDGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGLY 240
Query: 808 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 987
RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD
Sbjct: 241 RYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDRD 300
Query: 988 YGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 1167
Y ILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK
Sbjct: 301 YXILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREAK 360
Query: 1168 ECIYVEPDREGDKKGVYWYNNKL 1236
ECIYVEPDREGDKKGVYWYNNKL
Sbjct: 361 ECIYVEPDREGDKKGVYWYNNKL 383
>gb|AAD19742.1| delta-12 desaturase [Brassica carinata]
Length = 384
Score = 739 bits (1907), Expect = 0.0
Identities = 341/384 (88%), Positives = 358/384 (92%), Gaps = 1/384 (0%)
Frame = +1
Query: 88 MGAGGRMPVPXXXXXXXXXXXXRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 267
MGAGGRM V RVPCE PPF+VG+LKKAIPPHCFKRSIPRSFSYLI DI
Sbjct: 1 MGAGGRMQVSPSPKKSETDTIKRVPCETPPFTVGELKKAIPPHCFKRSIPRSFSYLIWDI 60
Query: 268 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 447
I+ASCFYYVAT YF LLP PLSY+AWPLYWACQGCVLTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61 IVASCFYYVATTYFPLLPHPLSYIAWPLYWACQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 448 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 627
TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS IKWYGKYLNNPLGR +
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSDIKWYGKYLNNPLGRTV 180
Query: 628 MLTVQFVLGWPLYLAFNVSGRPY-DGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGL 804
MLTVQF LGWPLYLAFNVSGRPY +GFACHF PNAPIYNDRERLQIY+SDAGILAVC+GL
Sbjct: 181 MLTVQFTLGWPLYLAFNVSGRPYPEGFACHFHPNAPIYNDRERLQIYVSDAGILAVCYGL 240
Query: 805 YRYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 984
YRYAAAQG+ASM+CLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR
Sbjct: 241 YRYAAAQGVASMVCLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 300
Query: 985 DYGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREA 1164
DYGILNKVFHNITDTHVAHHLFSTMPHY+AMEATKAIKPILGDYYQFDGTPW AM+REA
Sbjct: 301 DYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGDYYQFDGTPWVKAMWREA 360
Query: 1165 KECIYVEPDREGDKKGVYWYNNKL 1236
KECIYVEPDR+G+KKGV+WYNNKL
Sbjct: 361 KECIYVEPDRQGEKKGVFWYNNKL 384
>sp|Q39287|FD6E_BRAJU Omega-6 fatty acid desaturase, endoplasmic reticulum (Delta-12
desaturase)|gi|1212781|emb|CAA62578.1| oleate desaturase
[Brassica juncea]
Length = 384
Score = 731 bits (1886), Expect = 0.0
Identities = 338/384 (88%), Positives = 355/384 (92%), Gaps = 1/384 (0%)
Frame = +1
Query: 88 MGAGGRMPVPXXXXXXXXXXXXRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 267
MGAGGRM V RVPCE PPF+VG+LKKAIPPHCFKRSIPRSFSYLI DI
Sbjct: 1 MGAGGRMQVSPSPKKSETDTLKRVPCETPPFTVGELKKAIPPHCFKRSIPRSFSYLIWDI 60
Query: 268 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 447
I+ASCFYYVAT YF LLP PLSY+AWPLYWACQG VLTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61 IVASCFYYVATTYFPLLPHPLSYVAWPLYWACQGVVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 448 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 627
TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS IKWYGKYLNNPLGR +
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSDIKWYGKYLNNPLGRTV 180
Query: 628 MLTVQFVLGWPLYLAFNVSGRPY-DGFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGL 804
MLTVQF LGWPLY AFNVSGRPY +GFACHF PNAPIYNDRERLQIY+SDAGILAVC+GL
Sbjct: 181 MLTVQFTLGWPLYWAFNVSGRPYPEGFACHFHPNAPIYNDRERLQIYVSDAGILAVCYGL 240
Query: 805 YRYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 984
YRYAAAQG+ASM+CLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR
Sbjct: 241 YRYAAAQGVASMVCLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 300
Query: 985 DYGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREA 1164
DYGILNKVFHNITDTHVAHHLFSTMPHY+AME TKAIKPILGDYYQFDGTPW AM+REA
Sbjct: 301 DYGILNKVFHNITDTHVAHHLFSTMPHYHAMEVTKAIKPILGDYYQFDGTPWVKAMWREA 360
Query: 1165 KECIYVEPDREGDKKGVYWYNNKL 1236
KECIYVEPDR+G+KKGV+WYNNKL
Sbjct: 361 KECIYVEPDRQGEKKGVFWYNNKL 384
>emb|CAD30827.1| fatty acid desaturase 2 [Brassica rapa]
Length = 384
Score = 728 bits (1880), Expect = 0.0
Identities = 338/384 (88%), Positives = 353/384 (91%), Gaps = 1/384 (0%)
Frame = +1
Query: 88 MGAGGRMPVPXXXXXXXXXXXXRVPCEKPPFSVGDLKKAIPPHCFKRSIPRSFSYLISDI 267
MGAGGRM V RVPCE PPF+VG+LKKAIPPHCFKRSIPRSFSYLI DI
Sbjct: 1 MGAGGRMQVSPPSKKSETDNIKRVPCETPPFTVGELKKAIPPHCFKRSIPRSFSYLIWDI 60
Query: 268 IIASCFYYVATNYFSLLPQPLSYLAWPLYWACQGCVLTGIWVIAHECGHHAFSDYQWLDD 447
IIASCFYYVAT YF LLP PLSY AWPLYWACQGCVLTG+WVIAHECGHHAFSDYQWLDD
Sbjct: 61 IIASCFYYVATTYFPLLPHPLSYFAWPLYWACQGCVLTGVWVIAHECGHHAFSDYQWLDD 120
Query: 448 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKQKSAIKWYGKYLNNPLGRIM 627
TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPK+KS IKWYGKYLNNPLGR +
Sbjct: 121 TVGLIFHSFLLVPYFSWKYSHRRHHSNTGSLERDEVFVPKKKSDIKWYGKYLNNPLGRTV 180
Query: 628 MLTVQFVLGWPLYLAFNVSGRPYD-GFACHFFPNAPIYNDRERLQIYLSDAGILAVCFGL 804
MLTVQF LGWPLYLAFNVSGRPYD GFACHF PNAPIYNDRERLQIY+SDAGILAVC+GL
Sbjct: 181 MLTVQFTLGWPLYLAFNVSGRPYDGGFACHFHPNAPIYNDRERLQIYISDAGILAVCYGL 240
Query: 805 YRYAAAQGMASMICLYGVPLLIVNAFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 984
YRYAA QG+ASM+C YGVPLLIVN FLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR
Sbjct: 241 YRYAAVQGVASMVCFYGVPLLIVNGFLVLITYLQHTHPSLPHYDSSEWDWLRGALATVDR 300
Query: 985 DYGILNKVFHNITDTHVAHHLFSTMPHYNAMEATKAIKPILGDYYQFDGTPWYVAMYREA 1164
DYGILNKVFHNITDTHVAHHLFSTMPHY+AMEATKAIKPILG+YYQFDGTP AM+REA
Sbjct: 301 DYGILNKVFHNITDTHVAHHLFSTMPHYHAMEATKAIKPILGEYYQFDGTPVVKAMWREA 360
Query: 1165 KECIYVEPDREGDKKGVYWYNNKL 1236
KECIYVEPDR+G+KKGV+WYNNKL
Sbjct: 361 KECIYVEPDRQGEKKGVFWYNNKL 384
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 128
effective length of database: 257,790,653
effective search space used: 99764982711
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)