BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.sd.493.C1
(1119 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||S20866 L-ascorbate peroxidase (EC 1.11.1.11) precursor... 477 e-133
ref|NP_172267.1| ascorbate peroxidase, putative (APX); prot... 452 e-126
gb|AAN60795.1| ascorbate peroxidase [Brassica juncea] 436 e-121
gb|AAN60794.1| ascorbate peroxidase [Brassica juncea] 434 e-120
dbj|BAB84009.1| ascorbate peroxidase [Brassica oleracea] 432 e-120
>pir||S20866 L-ascorbate peroxidase (EC 1.11.1.11) precursor - Arabidopsis
thaliana (fragment)
Length = 263
Score = 477 bits (1228), Expect = e-133
Identities = 234/263 (88%), Positives = 234/263 (88%)
Frame = +3
Query: 105 DFIILPRNKSLAKMTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTF 284
DFIILPRNKSLAKMTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTF
Sbjct: 1 DFIILPRNKSLAKMTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTF 60
Query: 285 DCQSRTGGPFGTMRFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEV 464
DCQSRTGGPFGTMRFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEV
Sbjct: 61 DCQSRTGGPFGTMRFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEV 120
Query: 465 TGGPDIPFHPGREDKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGR 644
TGGPDIPFHPGREDKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGR
Sbjct: 121 TGGPDIPFHPGREDKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGR 180
Query: 645 CHKDRSGFEGAWTSNPLIFDNSYFXXXXXXXXXXXXXXVSDKALLDDPVFRPLVEKXXXX 824
CHKDRSGFEGAWTSNPLIFDNSYF VSDKALLDDPVFRPLVEK
Sbjct: 181 CHKDRSGFEGAWTSNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAAD 240
Query: 825 XXXXXXXXXXXHMKLSELGFADA 893
HMKLSELGFADA
Sbjct: 241 EDAFFADYAEAHMKLSELGFADA 263
>ref|NP_172267.1| ascorbate peroxidase, putative (APX); protein id: At1g07890.1,
supported by cDNA: 25057., supported by cDNA:
gi_14532509, supported by cDNA: gi_15912234, supported
by cDNA: gi_20334803 [Arabidopsis
thaliana]|gi|728873|sp|Q05431|APX1_ARATH L-ascorbate
peroxidase, cytosolic (AP)|gi|25285569|pir||D86214
hypothetical protein [imported] - Arabidopsis
thaliana|gi|16173|emb|CAA42168.1| L-ascorbate peroxidase
[Arabidopsis thaliana]|gi|217833|dbj|BAA03334.1|
ascorbate peroxidase [Arabidopsis
thaliana]|gi|1532170|gb|AAB07880.1| ascorbate peroxidase
[Arabidopsis
thaliana]|gi|8439880|gb|AAF75066.1|AC007583_2 Strong
similarity to L-ascorbate peroxidase from Arabidopsis
thaliana gi|728873. ESTs gb|T04087, gb|H37385,gb|H36515
and gb|R90494 come from this
gene|gi|14532510|gb|AAK63983.1| At1g07890/F24B9_2
[Arabidopsis thaliana]|gi|15912235|gb|AAL08251.1|
At1g07890/F24B9_2 [Arabidopsis
thaliana]|gi|20334804|gb|AAM16263.1| At1g07890/F24B9_2
[Arabidopsis thaliana]|gi|21554322|gb|AAM63427.1|
L-ascorbate peroxidase [Arabidopsis thaliana]
Length = 250
Score = 452 bits (1164), Expect = e-126
Identities = 221/250 (88%), Positives = 221/250 (88%)
Frame = +3
Query: 144 MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 323
MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM
Sbjct: 1 MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 60
Query: 324 RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 503
RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE
Sbjct: 61 RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 120
Query: 504 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 683
DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180
Query: 684 SNPLIFDNSYFXXXXXXXXXXXXXXVSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHM 863
SNPLIFDNSYF VSDKALLDDPVFRPLVEK HM
Sbjct: 181 SNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEDAFFADYAEAHM 240
Query: 864 KLSELGFADA 893
KLSELGFADA
Sbjct: 241 KLSELGFADA 250
>gb|AAN60795.1| ascorbate peroxidase [Brassica juncea]
Length = 250
Score = 436 bits (1121), Expect = e-121
Identities = 210/250 (84%), Positives = 217/250 (86%)
Frame = +3
Query: 144 MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 323
MTK+YPTVSEDY+KA+EKC+RKLRGLIAEKNCAPIMVRLAWHSAGTFDC S+TGGPFGTM
Sbjct: 1 MTKSYPTVSEDYQKAIEKCKRKLRGLIAEKNCAPIMVRLAWHSAGTFDCASKTGGPFGTM 60
Query: 324 RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 503
RFDAEQ HGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGP+IPFHPGRE
Sbjct: 61 RFDAEQGHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPEIPFHPGRE 120
Query: 504 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 683
DKPQPPPEGRLPDATKGCDHLR VF KQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRQVFTKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180
Query: 684 SNPLIFDNSYFXXXXXXXXXXXXXXVSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHM 863
SNPLIFDNSYF VSDKALLDDPVFRPLVEK H+
Sbjct: 181 SNPLIFDNSYFKELLTGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEEAFFADYAEAHL 240
Query: 864 KLSELGFADA 893
KLSELGFADA
Sbjct: 241 KLSELGFADA 250
>gb|AAN60794.1| ascorbate peroxidase [Brassica juncea]
Length = 250
Score = 434 bits (1116), Expect = e-120
Identities = 209/250 (83%), Positives = 216/250 (85%)
Frame = +3
Query: 144 MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 323
MTK+YPTVSEDY+KA+EKC+RKLRGLIAEKNCAPIMVRLAWHSAGTFDC S+TGGPFGTM
Sbjct: 1 MTKSYPTVSEDYQKAIEKCKRKLRGLIAEKNCAPIMVRLAWHSAGTFDCASKTGGPFGTM 60
Query: 324 RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 503
RFDAEQ HGANSGIHIALRLLDPIREQFP ISFADFHQLAGVVAVEVTGGP+IPFHPGRE
Sbjct: 61 RFDAEQGHGANSGIHIALRLLDPIREQFPAISFADFHQLAGVVAVEVTGGPEIPFHPGRE 120
Query: 504 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 683
DKPQPPPEGRLPDATKGCDHLR VF KQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRQVFTKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180
Query: 684 SNPLIFDNSYFXXXXXXXXXXXXXXVSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHM 863
SNPLIFDNSYF VSDKALLDDPVFRPLVEK H+
Sbjct: 181 SNPLIFDNSYFKELLTGEKEGLLQLVSDKALLDDPVFRPLVEKYAADEEAFFADYAEAHL 240
Query: 864 KLSELGFADA 893
KLSELGFADA
Sbjct: 241 KLSELGFADA 250
>dbj|BAB84009.1| ascorbate peroxidase [Brassica oleracea]
Length = 250
Score = 432 bits (1111), Expect = e-120
Identities = 208/250 (83%), Positives = 216/250 (86%)
Frame = +3
Query: 144 MTKNYPTVSEDYKKAVEKCRRKLRGLIAEKNCAPIMVRLAWHSAGTFDCQSRTGGPFGTM 323
MTK+YPTV+EDY+ A+EKC+RKLRGLIAEKNCAPIMVRLAWHSAGTFDC SRTGGPFGTM
Sbjct: 1 MTKSYPTVTEDYQNAIEKCKRKLRGLIAEKNCAPIMVRLAWHSAGTFDCASRTGGPFGTM 60
Query: 324 RFDAEQAHGANSGIHIALRLLDPIREQFPTISFADFHQLAGVVAVEVTGGPDIPFHPGRE 503
RFDAEQ HGANSGIHIALRLL+PIREQFPTISFADFHQLAGVVAVEVTGGP+IPFHPGRE
Sbjct: 61 RFDAEQGHGANSGIHIALRLLEPIREQFPTISFADFHQLAGVVAVEVTGGPEIPFHPGRE 120
Query: 504 DKPQPPPEGRLPDATKGCDHLRDVFAKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 683
DKPQPPPEGRLPDATKGCDHLR VF KQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT
Sbjct: 121 DKPQPPPEGRLPDATKGCDHLRQVFTKQMGLSDKDIVALSGAHTLGRCHKDRSGFEGAWT 180
Query: 684 SNPLIFDNSYFXXXXXXXXXXXXXXVSDKALLDDPVFRPLVEKXXXXXXXXXXXXXXXHM 863
SNPLIFDNSYF VSDKALLDDPVFRPLVEK H+
Sbjct: 181 SNPLIFDNSYFKELLSGEKEGLLQLVSDKALLDDPVFRPLVEKYADDEEAFFADYAEAHL 240
Query: 864 KLSELGFADA 893
KLSELGFADA
Sbjct: 241 KLSELGFADA 250
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 124
effective length of database: 263,146,837
effective search space used: 65260415576
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)