BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.sd.214.C1
(1630 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_171884.1| putative cruciferin 12S seed storage prote... 834 0.0
pir||S08510 cruciferin precursor (CRB) - Arabidopsis thalia... 832 0.0
pir||S14762 cruciferin 4 precursor - rape 727 0.0
sp|P33522|CRU4_BRANA CRUCIFERIN CRU4 PRECURSOR (11S GLOBULI... 722 0.0
emb|CAA40980.1| cruciferin cru4 subunit [Brassica napus] 659 0.0
>ref|NP_171884.1| putative cruciferin 12S seed storage protein; protein id:
At1g03880.1, supported by cDNA: gi_20260211 [Arabidopsis
thaliana]|gi|21542382|sp|P15456|12S2_ARATH 12S seed
storage protein precursor|gi|25296169|pir||E86169 12S
seed storage protein [imported] - Arabidopsis
thaliana|gi|4204299|gb|AAD10680.1| 12S seed storage
protein [Arabidopsis thaliana]|gi|20260212|gb|AAM13004.1|
12S seed storage protein [Arabidopsis thaliana]
Length = 455
Score = 834 bits (2155), Expect = 0.0
Identities = 413/455 (90%), Positives = 413/455 (90%)
Frame = +3
Query: 33 MGRVXXXXXXXXXXXXXXNGYTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQ 212
MGRV NGYTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQ
Sbjct: 1 MGRVSSIISFSLTLLILFNGYTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQ 60
Query: 213 LRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMESPVFXXXX 392
LRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMESPVF
Sbjct: 61 LRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMESPVFGEGQ 120
Query: 393 XXXXXXXFRDMHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRN 572
FRDMHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRN
Sbjct: 121 GQGQSQGFRDMHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRN 180
Query: 573 LRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGN 752
LRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGN
Sbjct: 181 LRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGN 240
Query: 753 IVKVNGPFGVIRPPLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYI 932
IVKVNGPFGVIRPPLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYI
Sbjct: 241 IVKVNGPFGVIRPPLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYI 300
Query: 933 STLNSYNXXXXXXXXXXXXXXXXXKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERV 1112
STLNSYN KNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERV
Sbjct: 301 STLNSYNLPILRLLRLSALRGSIRKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERV 360
Query: 1113 FDQEISSGQLLVVPQGFSVMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVIT 1292
FDQEISSGQLLVVPQGFSVMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVIT
Sbjct: 361 FDQEISSGQLLVVPQGFSVMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVIT 420
Query: 1293 NGYQISPEEAKRVKFSTIETTLTHSSPMSYGRPRA 1397
NGYQISPEEAKRVKFSTIETTLTHSSPMSYGRPRA
Sbjct: 421 NGYQISPEEAKRVKFSTIETTLTHSSPMSYGRPRA 455
>pir||S08510 cruciferin precursor (CRB) - Arabidopsis
thaliana|gi|166678|gb|AAA32778.1| 12S storage protein
CRB|gi|808937|emb|CAA32494.1| 12S seed storage protein
[Arabidopsis thaliana]
Length = 455
Score = 832 bits (2150), Expect = 0.0
Identities = 412/455 (90%), Positives = 412/455 (90%)
Frame = +3
Query: 33 MGRVXXXXXXXXXXXXXXNGYTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQ 212
MGRV NGYTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQ
Sbjct: 1 MGRVSSIISFSLTLLILFNGYTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQ 60
Query: 213 LRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMESPVFXXXX 392
LRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMESPVF
Sbjct: 61 LRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMESPVFGEGQ 120
Query: 393 XXXXXXXFRDMHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRN 572
FRDMHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRN
Sbjct: 121 GQGQSQGFRDMHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRN 180
Query: 573 LRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGN 752
LRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGN
Sbjct: 181 LRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGN 240
Query: 753 IVKVNGPFGVIRPPLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYI 932
IVKVNGPFGVIRPPLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYI
Sbjct: 241 IVKVNGPFGVIRPPLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYI 300
Query: 933 STLNSYNXXXXXXXXXXXXXXXXXKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERV 1112
STLNSYN KNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERV
Sbjct: 301 STLNSYNLPILRLLRLSALRGSIRKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERV 360
Query: 1113 FDQEISSGQLLVVPQGFSVMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVIT 1292
FDQEISSGQLLVVPQGFSVMKH IGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVIT
Sbjct: 361 FDQEISSGQLLVVPQGFSVMKHRIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVIT 420
Query: 1293 NGYQISPEEAKRVKFSTIETTLTHSSPMSYGRPRA 1397
NGYQISPEEAKRVKFSTIETTLTHSSPMSYGRPRA
Sbjct: 421 NGYQISPEEAKRVKFSTIETTLTHSSPMSYGRPRA 455
>pir||S14762 cruciferin 4 precursor - rape
Length = 465
Score = 727 bits (1877), Expect = 0.0
Identities = 358/445 (80%), Positives = 385/445 (86%), Gaps = 9/445 (2%)
Frame = +3
Query: 87 NGYTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGL 266
+G+TAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGL
Sbjct: 18 HGFTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGL 77
Query: 267 FLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMESPVFXXXXXXXXXXX--------FRD 422
+LPTFLNAGKLTFVVHG LMG+V PGCAETFM+SPVF FRD
Sbjct: 78 YLPTFLNAGKLTFVVHGHALMGKVTPGCAETFMDSPVFGQGQGQEQGQGQGQGQGQGFRD 137
Query: 423 MHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNN 602
MHQKVEHLR GDTIATP GVAQWFYNNGNEPLILVAAAD+A+N NQLDRNLRPFL+AGNN
Sbjct: 138 MHQKVEHLRSGDTIATPPGVAQWFYNNGNEPLILVAAADIANNLNQLDRNLRPFLLAGNN 197
Query: 603 PQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGV 782
PQGQ+WLQGR+QQKQNNIFNGFAP+ILAQAFKI+VETAQ+LQNQQ NRGNIVKV G FGV
Sbjct: 198 PQGQQWLQGRQQQKQNNIFNGFAPQILAQAFKISVETAQKLQNQQVNRGNIVKVQGQFGV 257
Query: 783 IRPPLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNXXX 962
IRPPLR+G+GGQQP E NGLEETLCTMRCTENLDDPS ADVYKPSLGYISTLNSYN
Sbjct: 258 IRPPLRQGQGGQQPQEEGNGLEETLCTMRCTENLDDPSSADVYKPSLGYISTLNSYNLPI 317
Query: 963 XXXXXXXXXXXXXXKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERVFDQEISSGQL 1142
NAMVLPQWNVNANAALYVT GKAHIQMVNDNG+RVFDQEIS GQL
Sbjct: 318 LRFLRLSALRGSIHNNAMVLPQWNVNANAALYVTKGKAHIQMVNDNGQRVFDQEISKGQL 377
Query: 1143 LVVPQGFSVMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVITNGYQISPEEA 1322
LVVPQGF+V+K A +QF+WIEFK+N+NAQ+NTLAGRTSVMRGLPLEVI+NGYQISP+EA
Sbjct: 378 LVVPQGFAVVKRATSQQFQWIEFKSNDNAQINTLAGRTSVMRGLPLEVISNGYQISPQEA 437
Query: 1323 KRVKFSTIETTLTHSS-PMSYGRPR 1394
+ VKFST+ETTLT SS PM YG PR
Sbjct: 438 RSVKFSTLETTLTQSSGPMGYGMPR 462
>sp|P33522|CRU4_BRANA CRUCIFERIN CRU4 PRECURSOR (11S GLOBULIN) (12S STORAGE PROTEIN)
Length = 465
Score = 722 bits (1863), Expect = 0.0
Identities = 356/445 (80%), Positives = 383/445 (86%), Gaps = 9/445 (2%)
Frame = +3
Query: 87 NGYTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGL 266
+G+TAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGL
Sbjct: 18 HGFTAQQWPNECQLDQLNALEPSQIIKSEGGRIEVWDHHAPQLRCSGFAFERFVIEPQGL 77
Query: 267 FLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMESPVFXXXXXXXXXXX--------FRD 422
+LPTFLNAGKLTFVVHG LMG+V PGCAETF +SPVF FRD
Sbjct: 78 YLPTFLNAGKLTFVVHGHALMGKVTPGCAETFNDSPVFGQGQGQEQGQGQGQGQGQGFRD 137
Query: 423 MHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLILVAAADLASNQNQLDRNLRPFLIAGNN 602
MHQKVEHLR GDTIATP GVAQWFYNNGNEPLILVAAAD+A+N NQLDRNLRPFL+AGNN
Sbjct: 138 MHQKVEHLRSGDTIATPPGVAQWFYNNGNEPLILVAAADIANNLNQLDRNLRPFLLAGNN 197
Query: 603 PQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKINVETAQQLQNQQDNRGNIVKVNGPFGV 782
PQGQ+WLQGR+QQKQNNIFNGFAP+ILAQAFKI+VETAQ+LQNQQ NRGNIVKV G FGV
Sbjct: 198 PQGQQWLQGRQQQKQNNIFNGFAPQILAQAFKISVETAQKLQNQQVNRGNIVKVQGQFGV 257
Query: 783 IRPPLRRGEGGQQPHEIANGLEETLCTMRCTENLDDPSDADVYKPSLGYISTLNSYNXXX 962
IRPPLR+G+GGQQP E NGLEETLCTMRCTENLDDPS ADVYKPSLGYISTLNSYN
Sbjct: 258 IRPPLRQGQGGQQPQEEGNGLEETLCTMRCTENLDDPSSADVYKPSLGYISTLNSYNLPI 317
Query: 963 XXXXXXXXXXXXXXKNAMVLPQWNVNANAALYVTNGKAHIQMVNDNGERVFDQEISSGQL 1142
NAMVLPQWNVNANAALYVT GKAHIQ VNDNG+RVFDQEIS GQL
Sbjct: 318 LRFLRLSALRGSIHNNAMVLPQWNVNANAALYVTKGKAHIQNVNDNGQRVFDQEISKGQL 377
Query: 1143 LVVPQGFSVMKHAIGEQFEWIEFKTNENAQVNTLAGRTSVMRGLPLEVITNGYQISPEEA 1322
LVVPQGF+V+K A +QF+WIEFK+N+NAQ+NTLAGRTSVMRGLPLEVI+NGYQISP+EA
Sbjct: 378 LVVPQGFAVVKRATSQQFQWIEFKSNDNAQINTLAGRTSVMRGLPLEVISNGYQISPQEA 437
Query: 1323 KRVKFSTIETTLTHSS-PMSYGRPR 1394
+ VKFST+ETTLT SS PM YG PR
Sbjct: 438 RSVKFSTLETTLTQSSGPMGYGMPR 462
>emb|CAA40980.1| cruciferin cru4 subunit [Brassica napus]
Length = 413
Score = 659 bits (1699), Expect = 0.0
Identities = 325/410 (79%), Positives = 350/410 (85%), Gaps = 9/410 (2%)
Frame = +3
Query: 192 WDHHAPQLRCSGFAFERFVIEPQGLFLPTFLNAGKLTFVVHGRGLMGRVIPGCAETFMES 371
WDHHAPQLRCSGFAFERFVIEPQGL+LPTFLNAGKLTFVVHG LMG+V PGCAETFM+S
Sbjct: 1 WDHHAPQLRCSGFAFERFVIEPQGLYLPTFLNAGKLTFVVHGHALMGKVTPGCAETFMDS 60
Query: 372 PVFXXXXXXXXXXX--------FRDMHQKVEHLRCGDTIATPSGVAQWFYNNGNEPLILV 527
PVF FRDMHQKVEHLR GDTIATP GVAQWFYNNGNEPLILV
Sbjct: 61 PVFGQGQGQEQGQGQGQGQGQGFRDMHQKVEHLRSGDTIATPPGVAQWFYNNGNEPLILV 120
Query: 528 AAADLASNQNQLDRNLRPFLIAGNNPQGQEWLQGRKQQKQNNIFNGFAPEILAQAFKINV 707
AAAD+A+N NQLDRNLRPFL+AGNNPQGQ+WLQGR+QQKQNNIFNGFAP+ILAQAFKI+V
Sbjct: 121 AAADIANNLNQLDRNLRPFLLAGNNPQGQQWLQGRQQQKQNNIFNGFAPQILAQAFKISV 180
Query: 708 ETAQQLQNQQDNRGNIVKVNGPFGVIRPPLRRGEGGQQPHEIANGLEETLCTMRCTENLD 887
ETAQ+LQNQQ NRGNIVKV G FGVIRPPLR+G+GGQQP E NGLEETLCTMRCTENLD
Sbjct: 181 ETAQKLQNQQVNRGNIVKVQGQFGVIRPPLRQGQGGQQPQEEGNGLEETLCTMRCTENLD 240
Query: 888 DPSDADVYKPSLGYISTLNSYNXXXXXXXXXXXXXXXXXKNAMVLPQWNVNANAALYVTN 1067
DPS ADVYKPSLGYISTLNSYN NAMVLPQWNVNANAALYVT
Sbjct: 241 DPSSADVYKPSLGYISTLNSYNLPILRFLRLSALRGSIHNNAMVLPQWNVNANAALYVTK 300
Query: 1068 GKAHIQMVNDNGERVFDQEISSGQLLVVPQGFSVMKHAIGEQFEWIEFKTNENAQVNTLA 1247
GKAHIQMVNDNG+RVFDQEIS GQLLVVPQGF+V+K A +QF+WIEFK+N+NAQ+NTLA
Sbjct: 301 GKAHIQMVNDNGQRVFDQEISKGQLLVVPQGFAVVKRATSQQFQWIEFKSNDNAQINTLA 360
Query: 1248 GRTSVMRGLPLEVITNGYQISPEEAKRVKFSTIETTLTHSS-PMSYGRPR 1394
GRTSVMRGLPLEVI+NGYQISP+EA+ VKFST+ETTLT SS PM YG PR
Sbjct: 361 GRTSVMRGLPLEVISNGYQISPQEARSVKFSTLETTLTQSSGPMGYGMPR 410
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 128
effective length of database: 257,790,653
effective search space used: 106725330342
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)