BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.sd.17503.C1
(1139 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_569050.1| adenylylsulfate kinase-like protein; prote... 532 e-163
gb|AAM61589.1| adenylylsulfate kinase-like protein [Arabido... 521 e-160
dbj|BAB08460.1| adenylylsulfate kinase-like protein [Arabid... 506 e-154
sp|O49204|KAPS_CATRO Adenylylsulfate kinase, chloroplast pr... 292 6e-82
ref|NP_195704.1| adenosine-5-phosphosulfate-kinase; protein... 296 1e-79
>ref|NP_569050.1| adenylylsulfate kinase-like protein; protein id: At5g67520.1,
supported by cDNA: 125039. [Arabidopsis
thaliana]|gi|28393175|gb|AAO42019.1| putative
adenylylsulfate kinase [Arabidopsis thaliana]
Length = 310
Score = 532 bits (1370), Expect(2) = e-163
Identities = 264/264 (100%), Positives = 264/264 (100%)
Frame = +2
Query: 101 MDVAAMARCVGRCYVSPAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMS 280
MDVAAMARCVGRCYVSPAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMS
Sbjct: 1 MDVAAMARCVGRCYVSPAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMS 60
Query: 281 YFRPIMAKDESISSRSGETKQINGKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGS 460
YFRPIMAKDESISSRSGETKQINGKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGS
Sbjct: 61 YFRPIMAKDESISSRSGETKQINGKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGS 120
Query: 461 GKSSLACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADS 640
GKSSLACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADS
Sbjct: 121 GKSSLACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADS 180
Query: 641 GIICIASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGF 820
GIICIASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGF
Sbjct: 181 GIICIASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGF 240
Query: 821 TGVDDPYEAPLDCEIVIQNSRDKG 892
TGVDDPYEAPLDCEIVIQNSRDKG
Sbjct: 241 TGVDDPYEAPLDCEIVIQNSRDKG 264
Score = 68.2 bits (165), Expect(2) = e-163
Identities = 31/48 (64%), Positives = 32/48 (66%)
Frame = +3
Query: 888 RGLXXXXXXXXXXXXXXXXLCEMADIVVSYLDQNGYLKKHSTKSRNCM 1031
+GL LCEMADIVVSYLDQNGYLKKHSTKSRNCM
Sbjct: 263 KGLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLKKHSTKSRNCM 310
>gb|AAM61589.1| adenylylsulfate kinase-like protein [Arabidopsis thaliana]
Length = 305
Score = 521 bits (1342), Expect(2) = e-160
Identities = 258/259 (99%), Positives = 258/259 (99%)
Frame = +2
Query: 116 MARCVGRCYVSPAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMSYFRPI 295
MARCVGRCYVSPAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMSYFRPI
Sbjct: 1 MARCVGRCYVSPAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMSYFRPI 60
Query: 296 MAKDESISSRSGETKQINGKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSL 475
MAKDESISSRSGETKQINGKQKNIVWHDCPVTKSDRQELIK KGCVIWITGLSGSGKSSL
Sbjct: 61 MAKDESISSRSGETKQINGKQKNIVWHDCPVTKSDRQELIKHKGCVIWITGLSGSGKSSL 120
Query: 476 ACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICI 655
ACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICI
Sbjct: 121 ACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICI 180
Query: 656 ASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDD 835
ASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDD
Sbjct: 181 ASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDD 240
Query: 836 PYEAPLDCEIVIQNSRDKG 892
PYEAPLDCEIVIQNSRDKG
Sbjct: 241 PYEAPLDCEIVIQNSRDKG 259
Score = 66.2 bits (160), Expect(2) = e-160
Identities = 30/48 (62%), Positives = 32/48 (66%)
Frame = +3
Query: 888 RGLXXXXXXXXXXXXXXXXLCEMADIVVSYLDQNGYLKKHSTKSRNCM 1031
+GL LCEMADIVVSYLDQNGYLKKHSTKSR+CM
Sbjct: 258 KGLSSSSSSSSSPSSSSSSLCEMADIVVSYLDQNGYLKKHSTKSRDCM 305
>dbj|BAB08460.1| adenylylsulfate kinase-like protein [Arabidopsis thaliana]
Length = 290
Score = 506 bits (1302), Expect(2) = e-154
Identities = 250/252 (99%), Positives = 251/252 (99%)
Frame = +2
Query: 116 MARCVGRCYVSPAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMSYFRPI 295
MARCVGRCYVSPAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMSYFRPI
Sbjct: 1 MARCVGRCYVSPAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMSYFRPI 60
Query: 296 MAKDESISSRSGETKQINGKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSL 475
MAKDESISSRSGETKQINGKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSL
Sbjct: 61 MAKDESISSRSGETKQINGKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSL 120
Query: 476 ACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICI 655
ACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICI
Sbjct: 121 ACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICI 180
Query: 656 ASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDD 835
ASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDD
Sbjct: 181 ASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDD 240
Query: 836 PYEAPLDCEIVI 871
PYEAPLDCE+ I
Sbjct: 241 PYEAPLDCEVHI 252
Score = 64.3 bits (155), Expect(2) = e-154
Identities = 29/29 (100%), Positives = 29/29 (100%)
Frame = +3
Query: 945 LCEMADIVVSYLDQNGYLKKHSTKSRNCM 1031
LCEMADIVVSYLDQNGYLKKHSTKSRNCM
Sbjct: 262 LCEMADIVVSYLDQNGYLKKHSTKSRNCM 290
>sp|O49204|KAPS_CATRO Adenylylsulfate kinase, chloroplast precursor (APS kinase)
(Adenosine-5'phosphosulfate kinase) (ATP
adenosine-5'-phosphosulfate
3'-phosphotransferase)|gi|7434264|pir||T08076
adenylyl-sulfate kinase (EC 2.7.1.25) precursor -
Madagascar periwinkle|gi|2832300|gb|AAC31145.1|
adenosine-5'-phosphosulfate-kinase [Catharanthus roseus]
Length = 312
Score = 292 bits (747), Expect(2) = 6e-82
Identities = 139/174 (79%), Positives = 156/174 (88%)
Frame = +2
Query: 350 GKQKNIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKSSLACALSRALHNRGKLSYIL 529
G NI+WH C V KS+RQE ++Q+GCVIWITGLSGSGKS+LACALSR LH +GKL+YIL
Sbjct: 110 GNSTNILWHKCAVEKSERQEPLQQRGCVIWITGLSGSGKSTLACALSRGLHAKGKLTYIL 169
Query: 530 DGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGIICIASLISPYRIERAACRALL 709
DGDNVRHGLNSDLSF+A+DRAENIRR+GEVAKLFAD+G+ICIASLISPYR ACR+LL
Sbjct: 170 DGDNVRHGLNSDLSFKAEDRAENIRRIGEVAKLFADAGVICIASLISPYRKPPDACRSLL 229
Query: 710 PQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGVDDPYEAPLDCEIVI 871
P+GDFIEVFMDVPL VCEARDPKGLYK ARAGKIKGFTG+DDPYE PL EIV+
Sbjct: 230 PEGDFIEVFMDVPLKVCEARDPKGLYKLARAGKIKGFTGIDDPYEPPLKSEIVL 283
Score = 35.8 bits (81), Expect(2) = 6e-82
Identities = 13/18 (72%), Positives = 18/18 (99%)
Frame = +3
Query: 948 CEMADIVVSYLDQNGYLK 1001
C++ADIV+SYL++NGYLK
Sbjct: 294 CDLADIVISYLEENGYLK 311
>ref|NP_195704.1| adenosine-5-phosphosulfate-kinase; protein id: At4g39940.1,
supported by cDNA: gi_18087562, supported by cDNA:
gi_20453396, supported by cDNA: gi_2829132 [Arabidopsis
thaliana]|gi|7387808|sp|O49196|KAP2_ARATH
Adenylylsulfate kinase 2, chloroplast precursor (APS
kinase) (Adenosine-5'phosphosulfate kinase) (ATP
adenosine-5'-phosphosulfate
3'-phosphotransferase)|gi|7434263|pir||T06100
adenylyl-sulfate kinase (EC 2.7.1.25) [validated] -
Arabidopsis thaliana|gi|2829133|gb|AAC39520.1|
adenosine-5'-phosphosulfate-kinase [Arabidopsis
thaliana]|gi|4490745|emb|CAB38907.1|
adenosine-5'-phosphosulfate-kinase [Arabidopsis
thaliana]|gi|7271049|emb|CAB80657.1|
adenosine-5'-phosphosulfate-kinase [Arabidopsis
thaliana]|gi|18087563|gb|AAL58913.1|AF462823_1
AT4g39940/T5J17_110 [Arabidopsis
thaliana]|gi|20453397|gb|AAM19937.1| AT4g39940/T5J17_110
[Arabidopsis thaliana]
Length = 293
Score = 296 bits (759), Expect(2) = 1e-79
Identities = 153/259 (59%), Positives = 191/259 (73%), Gaps = 13/259 (5%)
Frame = +2
Query: 149 PAFGESESHRLSERRFLKLSSSTNSDPAGSKSLKLRGKIHRRMSYFRPIMAKDES----- 313
P G + FLKL +S S PA S+ K+ + F PI A + S
Sbjct: 18 PGLGGDSHRKPPSDGFLKLPAS--SIPADSR------KLVANSTSFHPISAVNVSAQASL 69
Query: 314 ------ISSRSGETKQINGKQK--NIVWHDCPVTKSDRQELIKQKGCVIWITGLSGSGKS 469
+S + + NGK+K NIVWH+ + + DRQ+L++QKGCV+WITGLSGSGKS
Sbjct: 70 TADFPALSETILKEGRNNGKEKAENIVWHESSICRCDRQQLLQQKGCVVWITGLSGSGKS 129
Query: 470 SLACALSRALHNRGKLSYILDGDNVRHGLNSDLSFEADDRAENIRRVGEVAKLFADSGII 649
++ACALS+AL RGKL+Y LDGDNVRHGLN DL+F+A+ R ENIRR+GEVAKLFAD G+I
Sbjct: 130 TVACALSKALFERGKLTYTLDGDNVRHGLNRDLTFKAEHRTENIRRIGEVAKLFADVGVI 189
Query: 650 CIASLISPYRIERAACRALLPQGDFIEVFMDVPLHVCEARDPKGLYKRARAGKIKGFTGV 829
CIASLISPYR +R ACR+LLP GDF+EVFMDVPLHVCE+RDPKGLYK ARAGKIKGFTG+
Sbjct: 190 CIASLISPYRRDRDACRSLLPDGDFVEVFMDVPLHVCESRDPKGLYKLARAGKIKGFTGI 249
Query: 830 DDPYEAPLDCEIVIQNSRD 886
DDPYEAP++CE+V++++ D
Sbjct: 250 DDPYEAPVNCEVVLKHTGD 268
Score = 23.5 bits (49), Expect(2) = 1e-79
Identities = 8/17 (47%), Positives = 13/17 (76%)
Frame = +3
Query: 951 EMADIVVSYLDQNGYLK 1001
+MA+ ++SYL GYL+
Sbjct: 276 QMAENIISYLQNKGYLE 292
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 125
effective length of database: 261,807,791
effective search space used: 66499178914
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)