BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.sd.17498.C1
(2949 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_201548.1| formin-like protein; protein id: At5g67470... 668 0.0
gb|AAN05367.1| Putative formin-like protein [Oryza sativa (... 414 e-138
dbj|BAB86073.1| putative formin-like protein AHF1 [Oryza sa... 374 e-134
ref|NP_189177.1| expressed protein; protein id: At3g25500.1... 377 e-129
gb|AAF24497.1|AF213696_1 FH protein NFH2 [Nicotiana tabacum] 354 e-121
>ref|NP_201548.1| formin-like protein; protein id: At5g67470.1 [Arabidopsis
thaliana]|gi|9757868|dbj|BAB08455.1| formin-like protein
[Arabidopsis thaliana]
Length = 899
Score = 668 bits (1723), Expect(2) = 0.0
Identities = 375/546 (68%), Positives = 375/546 (68%)
Frame = +1
Query: 1 MKALQXXXXXXXXXXXXXXXXXXXEAHRRILHQXXXXXXXXXXXXXXXXXXXXXXXXXXX 180
MKALQ EAHRRILHQ
Sbjct: 1 MKALQSRFFFFFFFYIFFSVSVSSEAHRRILHQPLFPESSTPPPPDFQSTPSPPLPDTPD 60
Query: 181 XXXXXENPSTPQQTLFXXXXXXVSADVNGGLPIPTATTQSAKPGKKVAIVISVGIVTLGM 360
ENPSTPQQTLF VSADVNGGLPIPTATTQSAKPGKKVAIVISVGIVTLGM
Sbjct: 61 QPFFPENPSTPQQTLFPPPPPPVSADVNGGLPIPTATTQSAKPGKKVAIVISVGIVTLGM 120
Query: 361 LSALAFFLYRHKAKHASDTQKLVTXXXXXXXSRRFQEDSGPPTTTSSTFLYMGTVEPTRV 540
LSALAFFLYRHKAKHASDTQKLVT SRRFQEDSGPPTTTSSTFLYMGTVEPTRV
Sbjct: 121 LSALAFFLYRHKAKHASDTQKLVTGGGDGGGSRRFQEDSGPPTTTSSTFLYMGTVEPTRV 180
Query: 541 SASESNGGTNGPVNSSPYRKLNSAKRSERYRPSXXXXXXXXXXXXXXXXXXXXXXXXXXX 720
SASESNGGTNGPVNSSPYRKLNSAKRSERYRPS
Sbjct: 181 SASESNGGTNGPVNSSPYRKLNSAKRSERYRPSPELQPLPPLAKPPQPSDNSPSALSPSS 240
Query: 721 XXXGEECRDTAFYTPHGSAISSDDGYYTAFPRSANGSLPHSKRTSPRSKFGSAPTTAASR 900
GEECRDTAFYTPHGSAISSDDGYYTAFPRSANGSLPHSKRTSPRSKFGSAPTTAASR
Sbjct: 241 SSSGEECRDTAFYTPHGSAISSDDGYYTAFPRSANGSLPHSKRTSPRSKFGSAPTTAASR 300
Query: 901 SPEMKHVIIPSIXXXXXXXXXXXXXRGLESDEQELPYXXXXXXXXXXXXXXXRAAFQAIT 1080
SPEMKHVIIPSI RGLESDEQELPY RAAFQAIT
Sbjct: 301 SPEMKHVIIPSIKQKLPPPVQPPPLRGLESDEQELPYSQNKPKFSQPPPPPNRAAFQAIT 360
Query: 1081 QEKSXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXDFQMLRKVXXXXXXXXXXXXX 1260
QEKS DFQMLRKV
Sbjct: 361 QEKSPVPPPRRSPPPLQTPPPPPPPPPLAPPPPPQKRPRDFQMLRKVTNSEATTNSTTSP 420
Query: 1261 XRKQAFKTPSPKTKAVEEVNSVSAGSLEKSGDGDTDPSKPKLKPLHWDKVRASSDRATVW 1440
RKQAFKTPSPKTKAVEEVNSVSAGSLEKSGDGDTDPSKPKLKPLHWDKVRASSDRATVW
Sbjct: 421 SRKQAFKTPSPKTKAVEEVNSVSAGSLEKSGDGDTDPSKPKLKPLHWDKVRASSDRATVW 480
Query: 1441 DQLKSSSFQLNEDRMEHLFGCNSGSSAPKEPVRRSVIPLAENENRVLDPKKSQNIAILLR 1620
DQLKSSSFQLNEDRMEHLFGCNSGSSAPKEPVRRSVIPLAENENRVLDPKKSQNIAILLR
Sbjct: 481 DQLKSSSFQLNEDRMEHLFGCNSGSSAPKEPVRRSVIPLAENENRVLDPKKSQNIAILLR 540
Query: 1621 ALNVTR 1638
ALNVTR
Sbjct: 541 ALNVTR 546
Score = 647 bits (1670), Expect(2) = 0.0
Identities = 337/337 (100%), Positives = 337/337 (100%)
Frame = +2
Query: 1643 EVSEALTDGNPESLGAELLETLVKMAPTKEEEIKLREYSGDVSKLGTAERFLKTILDIPF 1822
EVSEALTDGNPESLGAELLETLVKMAPTKEEEIKLREYSGDVSKLGTAERFLKTILDIPF
Sbjct: 548 EVSEALTDGNPESLGAELLETLVKMAPTKEEEIKLREYSGDVSKLGTAERFLKTILDIPF 607
Query: 1823 AFKRVEAMLYRANFDAEVKYLRNSFQTLEEASLELKASRLFLKLLEAVLMTGNRMNVGTN 2002
AFKRVEAMLYRANFDAEVKYLRNSFQTLEEASLELKASRLFLKLLEAVLMTGNRMNVGTN
Sbjct: 608 AFKRVEAMLYRANFDAEVKYLRNSFQTLEEASLELKASRLFLKLLEAVLMTGNRMNVGTN 667
Query: 2003 RGDAIAFKLDTLLKLVDIKGVDGKTTLLHFVVQEITRSEGTTTTKDETILHGNNDGFRKQ 2182
RGDAIAFKLDTLLKLVDIKGVDGKTTLLHFVVQEITRSEGTTTTKDETILHGNNDGFRKQ
Sbjct: 668 RGDAIAFKLDTLLKLVDIKGVDGKTTLLHFVVQEITRSEGTTTTKDETILHGNNDGFRKQ 727
Query: 2183 GLQVVAGLSRDLVNVKKSAGMDFDVLSSYVTKLEMGLDKLRSFLKTETTQGRFFDSMKTF 2362
GLQVVAGLSRDLVNVKKSAGMDFDVLSSYVTKLEMGLDKLRSFLKTETTQGRFFDSMKTF
Sbjct: 728 GLQVVAGLSRDLVNVKKSAGMDFDVLSSYVTKLEMGLDKLRSFLKTETTQGRFFDSMKTF 787
Query: 2363 LKEAEEEIRKIKGGERKALSMVKEVTEYFHGNAAREEAHPLRIFMVVRDFLGVLDNVCKE 2542
LKEAEEEIRKIKGGERKALSMVKEVTEYFHGNAAREEAHPLRIFMVVRDFLGVLDNVCKE
Sbjct: 788 LKEAEEEIRKIKGGERKALSMVKEVTEYFHGNAAREEAHPLRIFMVVRDFLGVLDNVCKE 847
Query: 2543 VKTMQEMSTSMGSASARSFRISATASLPVLHRYKARQ 2653
VKTMQEMSTSMGSASARSFRISATASLPVLHRYKARQ
Sbjct: 848 VKTMQEMSTSMGSASARSFRISATASLPVLHRYKARQ 884
>gb|AAN05367.1| Putative formin-like protein [Oryza sativa (japonica cultivar-group)]
Length = 849
Score = 414 bits (1063), Expect(2) = e-138
Identities = 224/337 (66%), Positives = 269/337 (79%), Gaps = 6/337 (1%)
Frame = +2
Query: 1643 EVSEALTDGNPESLGAELLETLVKMAPTKEEEIKLREYSGDVSKLGTAERFLKTILDIPF 1822
EV++AL DGN E LGAELLETLVKMAPTKEEE+KLR+++GD+SKLG+AERFLK +LDIPF
Sbjct: 498 EVTDALLDGNAECLGAELLETLVKMAPTKEEELKLRDFTGDLSKLGSAERFLKAVLDIPF 557
Query: 1823 AFKRVEAMLYRANFDAEVKYLRNSFQTLEEASLELKASRLFLKLLEAVLMTGNRMNVGTN 2002
AFKRV+ MLYRANF+ EV YLR SFQTLE A +LK SRLFLKLLEAVL TGNRMNVGTN
Sbjct: 558 AFKRVDVMLYRANFENEVNYLRKSFQTLEAACDDLKGSRLFLKLLEAVLRTGNRMNVGTN 617
Query: 2003 RGDAIAFKLDTLLKLVDIKGVDGKTTLLHFVVQEITRSEGTTTTK---DETILHGNNDGF 2173
RG+A AFKLDTLLKL D+KG DGKTTLLHFVVQEI RSE + K + +
Sbjct: 618 RGEAKAFKLDTLLKLADVKGADGKTTLLHFVVQEIVRSEDAKSEKAPENHITNIAKVEQL 677
Query: 2174 RKQGLQVVAGLSRDLVNVKKSAGMDFDVLSSYVTKLEMGLDKLRSFLKTE--TTQG-RFF 2344
R+QGL+VV+GLS +L NVK++A MDFDVL YV+KLE GL K++S L+ E +QG FF
Sbjct: 678 RRQGLKVVSGLSTELGNVKRAATMDFDVLHGYVSKLEAGLGKIKSVLQLEKQCSQGVNFF 737
Query: 2345 DSMKTFLKEAEEEIRKIKGGERKALSMVKEVTEYFHGNAAREEAHPLRIFMVVRDFLGVL 2524
+M+ FLKEAE+EI +++ E+ AL VKE+TEYFHGNA +EEAHPLRIFMVVRDFL +L
Sbjct: 738 ATMREFLKEAEQEIEQVRHDEKAALGRVKEITEYFHGNAVKEEAHPLRIFMVVRDFLSML 797
Query: 2525 DNVCKEVKTMQEMSTSMGSASARSFRISATASLPVLH 2635
D+VC+EV Q+ T +G SARSFRISA +LP+L+
Sbjct: 798 DHVCREVS--QQDRTFVG--SARSFRISAANALPILN 830
Score = 102 bits (255), Expect(2) = e-138
Identities = 62/120 (51%), Positives = 76/120 (62%), Gaps = 3/120 (2%)
Frame = +1
Query: 1285 PSPKTKAVEEVNSVSAG--SLEKSGDGDTDPSKPKLKPLHWDKVRASSDRATVWDQLKSS 1458
P AV + N +A S+ G+ D +PKLKPLHWDKVR SSDR VWD+LK
Sbjct: 381 PRAPAMAVTKDNDATAATMSVRTRGEAAGDEPRPKLKPLHWDKVRTSSDRDMVWDRLK-- 438
Query: 1459 SFQLNEDRMEHLFGCNSGSSAPKEPVRRSV-IPLAENENRVLDPKKSQNIAILLRALNVT 1635
L+ED +E LF NS + AP+ + V +P + E RVLDPKK+QNIAILLRALNVT
Sbjct: 439 ---LDEDMIEVLFMNNSTAVAPRMDNPKKVGMPQFKQEERVLDPKKAQNIAILLRALNVT 495
>dbj|BAB86073.1| putative formin-like protein AHF1 [Oryza sativa (japonica
cultivar-group)]|gi|22093600|dbj|BAC06896.1| putative
formin-like protein AHF1 [Oryza sativa (japonica
cultivar-group)]
Length = 960
Score = 374 bits (960), Expect(2) = e-134
Identities = 206/345 (59%), Positives = 255/345 (73%), Gaps = 15/345 (4%)
Frame = +2
Query: 1643 EVSEALTDGNPESLGAELLETLVKMAPTKEEEIKLREYSGDVS--KLGTAERFLKTILDI 1816
+V +AL +GN E+ GAELLETL+KMAPTKEEEIKLRE+ + S KLG AE+FLK +LDI
Sbjct: 595 QVCDALCEGNTENFGAELLETLLKMAPTKEEEIKLREFKEETSPIKLGPAEKFLKAVLDI 654
Query: 1817 PFAFKRVEAMLYRANFDAEVKYLRNSFQTLEEASLELKASRLFLKLLEAVLMTGNRMNVG 1996
PFAFKRV+AMLY ANF++EV YL+ SF+TLE A EL+ SRLFLKLLEAVL TGNRMNVG
Sbjct: 655 PFAFKRVDAMLYIANFESEVNYLKKSFETLETACDELRNSRLFLKLLEAVLKTGNRMNVG 714
Query: 1997 TNRGDAIAFKLDTLLKLVDIKGVDGKTTLLHFVVQEITRSEGT-------TTTKDETILH 2155
TNRGDA AFKLDTLLKLVD+KG DGKTTLLHFVVQEI R+EG+ +T + +
Sbjct: 715 TNRGDAHAFKLDTLLKLVDVKGTDGKTTLLHFVVQEIIRTEGSHLSASNQSTPRTQANPL 774
Query: 2156 GNNDGFRKQGLQVVAGLSRDLVNVKKSAGMDFDVLSSYVTKLEMGLDKLRSFL------K 2317
+ +K GLQVVAGL +L NVKK+A MD DVLSSYV+KL G++K+ L K
Sbjct: 775 RDELECKKLGLQVVAGLGNELSNVKKAAAMDSDVLSSYVSKLAGGIEKITEVLRLNEEVK 834
Query: 2318 TETTQGRFFDSMKTFLKEAEEEIRKIKGGERKALSMVKEVTEYFHGNAAREEAHPLRIFM 2497
+ RF DSM+ FLK A+++I +++ E ALS+VKE+TEYFHG++A+EEAHP RIFM
Sbjct: 835 SREDAWRFHDSMQKFLKRADDDIIRVQAQESVALSLVKEITEYFHGDSAKEEAHPFRIFM 894
Query: 2498 VVRDFLGVLDNVCKEVKTMQEMSTSMGSASARSFRISATASLPVL 2632
VVRDFL VLD VCKEV + + + ++S R F + +P L
Sbjct: 895 VVRDFLSVLDQVCKEVGRINDRTI---ASSVRHFPVPVNPMMPQL 936
Score = 130 bits (328), Expect(2) = e-134
Identities = 70/112 (62%), Positives = 83/112 (73%), Gaps = 5/112 (4%)
Frame = +1
Query: 1318 NSVSAGSLEKSGDGDTDPS---KPKLKPLHWDKVRASSDRATVWDQLKSSSFQLNEDRME 1488
N+ A + G GD +PKLKPLHWDKVRASSDR VWDQLKSSSFQ+NE+ +E
Sbjct: 482 NADHAAAAAAGGGGDKSEETTPRPKLKPLHWDKVRASSDRVMVWDQLKSSSFQVNEEMIE 541
Query: 1489 HLFGCNSGSSA-PKEP-VRRSVIPLAENENRVLDPKKSQNIAILLRALNVTR 1638
LF CN +SA PKEP RR V+P + +N+VLDPKKSQNIAILLRALNV++
Sbjct: 542 TLFICNPANSAPPKEPATRRPVLPTPKTDNKVLDPKKSQNIAILLRALNVSK 593
>ref|NP_189177.1| expressed protein; protein id: At3g25500.1, supported by cDNA:
gi_19423898, supported by cDNA: gi_6503009 [Arabidopsis
thaliana]|gi|6503010|gb|AAF14548.1| formin-like protein
AHF1 [Arabidopsis thaliana]|gi|9279730|dbj|BAB01320.1|
formin-like protein [Arabidopsis
thaliana]|gi|19423899|gb|AAL87275.1| putative formin
protein AHF1 [Arabidopsis
thaliana]|gi|23296692|gb|AAN13148.1| putative formin
protein AHF1 [Arabidopsis thaliana]
Length = 1051
Score = 377 bits (968), Expect(2) = e-129
Identities = 206/335 (61%), Positives = 254/335 (75%), Gaps = 7/335 (2%)
Frame = +2
Query: 1643 EVSEALTDGNPESLGAELLETLVKMAPTKEEEIKLREYSGDVS-KLGTAERFLKTILDIP 1819
EV EAL +GN ++LG ELLE+L+KMAPTKEEE KL+ Y+ D KLG AE+FLK +LDIP
Sbjct: 686 EVCEALLEGNADTLGTELLESLLKMAPTKEEERKLKAYNDDSPVKLGHAEKFLKAMLDIP 745
Query: 1820 FAFKRVEAMLYRANFDAEVKYLRNSFQTLEEASLELKASRLFLKLLEAVLMTGNRMNVGT 1999
FAFKRV+AMLY ANF++EV+YL+ SF+TLE A EL+ SR+FLKLLEAVL TGNRMNVGT
Sbjct: 746 FAFKRVDAMLYVANFESEVEYLKKSFETLEAACEELRNSRMFLKLLEAVLKTGNRMNVGT 805
Query: 2000 NRGDAIAFKLDTLLKLVDIKGVDGKTTLLHFVVQEITRSEGTTTTKDETILHGNNDGFRK 2179
NRGDA AFKLDTLLKLVD+KG DGKTTLLHFVVQEI R+EGT + + T ++ RK
Sbjct: 806 NRGDAHAFKLDTLLKLVDVKGADGKTTLLHFVVQEIIRAEGTRLSGNNT--QTDDIKCRK 863
Query: 2180 QGLQVVAGLSRDLVNVKKSAGMDFDVLSSYVTKLEMGLDKL------RSFLKTETTQGRF 2341
GLQVV+ L +L NVKK+A MD +VLSSYV+KL G+ K+ +S + E+ RF
Sbjct: 864 LGLQVVSSLCSELSNVKKAAAMDSEVLSSYVSKLSQGIAKINEAIQVQSTITEESNSQRF 923
Query: 2342 FDSMKTFLKEAEEEIRKIKGGERKALSMVKEVTEYFHGNAAREEAHPLRIFMVVRDFLGV 2521
+SMKTFLK AEEEI +++ E ALS+VKE+TEYFHGN+A+EEAHP RIF+VVRDFLGV
Sbjct: 924 SESMKTFLKRAEEEIIRVQAQESVALSLVKEITEYFHGNSAKEEAHPFRIFLVVRDFLGV 983
Query: 2522 LDNVCKEVKTMQEMSTSMGSASARSFRISATASLP 2626
+D VCKEV + E + +SA F + +P
Sbjct: 984 VDRVCKEVGMINERTM---VSSAHKFPVPVNPMMP 1015
Score = 109 bits (272), Expect(2) = e-129
Identities = 57/89 (64%), Positives = 67/89 (75%), Gaps = 2/89 (2%)
Frame = +1
Query: 1375 KPKLKPLHWDKVRASSDRATVWDQLKSSSFQLNEDRMEHLFGCNSGSSAP--KEPVRRSV 1548
KPKLK LHWDKVRASSDR VWD L+SSSF+L+E+ +E LF S ++ P + R V
Sbjct: 595 KPKLKALHWDKVRASSDREMVWDHLRSSSFKLDEEMIETLFVAKSLNNKPNQSQTTPRCV 654
Query: 1549 IPLAENENRVLDPKKSQNIAILLRALNVT 1635
+P ENRVLDPKK+QNIAILLRALNVT
Sbjct: 655 LPSPNQENRVLDPKKAQNIAILLRALNVT 683
>gb|AAF24497.1|AF213696_1 FH protein NFH2 [Nicotiana tabacum]
Length = 835
Score = 354 bits (908), Expect(2) = e-121
Identities = 196/350 (56%), Positives = 256/350 (73%), Gaps = 13/350 (3%)
Frame = +2
Query: 1643 EVSEALTDGNPESLGAELLETLVKMAPTKEEEIKLREYSGDVS-KLGTAERFLKTILDIP 1819
E+ EA +GN E++G ELLE L+KMAP+KEEE KL+EY D KLG AE+FLK +LDIP
Sbjct: 477 EICEAFLEGNAENIGTELLEILLKMAPSKEEERKLKEYKDDSPFKLGPAEKFLKAVLDIP 536
Query: 1820 FAFKRVEAMLYRANFDAEVKYLRNSFQTLEEASLELKASRLFLKLLEAVLMTGNRMNVGT 1999
FAFKR++AMLY +NFD EV YL NSF+TLE A EL++SR+FLKLLEAVL TGNRMNVGT
Sbjct: 537 FAFKRIDAMLYISNFDYEVDYLGNSFETLEAACEELRSSRMFLKLLEAVLKTGNRMNVGT 596
Query: 2000 NRGDAIAFKLDTLLKLVDIKGVDGKTTLLHFVVQEITRSEGTTTT---KDETILHGNNDG 2170
NRGDA AFKLDTLLKLVD+KG DGKTTLLHFVVQEI +SEG + ++ N+D
Sbjct: 597 NRGDAHAFKLDTLLKLVDVKGADGKTTLLHFVVQEIIKSEGARLSGGNQNHQQSTTNDDA 656
Query: 2171 -FRKQGLQVVAGLSRDLVNVKKSAGMDFDVLSSYVTKLEMGLDKLRSFLKT-------ET 2326
+K GLQVV+ +S +L+NVKKSA MD +VL + V KL G+ + +++ E+
Sbjct: 657 KCKKLGLQVVSNISSELINVKKSAAMDSEVLHNDVLKLSKGIQNIAEVVRSIEAVGLEES 716
Query: 2327 TQGRFFDSMKTFLKEAEEEIRKIKGGERKALSMVKEVTEYFHGNAAREEAHPLRIFMVVR 2506
+ RF +SM F+K AEE+I +++ E A+S+VKE+TEY HG++AREEAHP RIFMVV+
Sbjct: 717 SIKRFSESMNRFMKVAEEKILRLQAQETLAMSLVKEITEYVHGDSAREEAHPFRIFMVVK 776
Query: 2507 DFLGVLDNVCKEVKTMQEMSTSMGSASARSFRISATASL-PVLHRYKARQ 2653
DFL +LD VCKEV T+ E + +SA+ F + +L PV+ ++A++
Sbjct: 777 DFLMILDCVCKEVGTINERTI---VSSAQKFPVPVNPNLQPVISGFRAKR 823
Score = 107 bits (268), Expect(2) = e-121
Identities = 58/105 (55%), Positives = 70/105 (66%)
Frame = +1
Query: 1321 SVSAGSLEKSGDGDTDPSKPKLKPLHWDKVRASSDRATVWDQLKSSSFQLNEDRMEHLFG 1500
+V S+EK+ + + KPKLK LHWDKVRASSD VWDQLKSSSF+LNE+ +E LF
Sbjct: 370 NVENHSIEKNEEKSEEILKPKLKTLHWDKVRASSDCEMVWDQLKSSSFKLNEEMIETLFV 429
Query: 1501 CNSGSSAPKEPVRRSVIPLAENENRVLDPKKSQNIAILLRALNVT 1635
+ + + V+ ENRVLDPKKSQNIAILLR LN T
Sbjct: 430 VKNPTLNTSATAKHFVVSSMSQENRVLDPKKSQNIAILLRVLNGT 474
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 133
effective length of database: 251,095,423
effective search space used: 213180014127
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)