BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AY132012.f
(882 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_171616.1| chloroplast RNA-binding protein cp33, puta... 461 e-129
gb|AAF26472.1|AC007323_13 T25K16.8 [Arabidopsis thaliana] 368 e-101
pir||S49463 RNA-binding protein RNP1 precursor - kidney bea... 117 3e-25
sp|P19684|ROC5_NICSY 33 kDa ribonucleoprotein, chloroplast ... 115 6e-25
pir||S77714 RNA-binding protein precursor, 33K - wood tobac... 115 1e-24
>ref|NP_171616.1| chloroplast RNA-binding protein cp33, putative; protein id:
At1g01080.1, supported by cDNA: 21739., supported by
cDNA: gi_16930400 [Arabidopsis
thaliana]|gi|16930401|gb|AAL31886.1|AF419554_1
At1g01080/T25K16_7 [Arabidopsis
thaliana]|gi|21554268|gb|AAM63343.1| ribonucleoprotein,
putative [Arabidopsis
thaliana]|gi|22135777|gb|AAM91045.1| At1g01080/T25K16_7
[Arabidopsis thaliana]
Length = 293
Score = 461 bits (1186), Expect = e-129
Identities = 237/293 (80%), Positives = 237/293 (80%)
Frame = +1
Query: 1 MAASCFAIPLSSSSRSSHNAIPXXXXXXXXXXXXXXXXXXXQXXXXXXXXXXXXXRPFVA 180
MAASCFAIPLSSSSRSSHNAIP Q RPFVA
Sbjct: 1 MAASCFAIPLSSSSRSSHNAIPKYKTLISSSSYSYLESLKLQFSSSNSFHHSSLSRPFVA 60
Query: 181 QPLQIXXXXXXXXXXXXXXXXXXXXXXXXPNKDSVVSKAEPVKKPRPCELYVCNIPRSYD 360
QPLQI PNKDSVVSKAEPVKKPRPCELYVCNIPRSYD
Sbjct: 61 QPLQIKVSSSELSVLDEEKEEEVVKGEAEPNKDSVVSKAEPVKKPRPCELYVCNIPRSYD 120
Query: 361 IAQLLDMFQPFGTVISVEVSRNPQTGESRGSGYVTMGSINSAKIAIASLDGTEVGGREMR 540
IAQLLDMFQPFGTVISVEVSRNPQTGESRGSGYVTMGSINSAKIAIASLDGTEVGGREMR
Sbjct: 121 IAQLLDMFQPFGTVISVEVSRNPQTGESRGSGYVTMGSINSAKIAIASLDGTEVGGREMR 180
Query: 541 VRYSVDMNPGTRRNPEVLNSTPKKILMYESQHKVYVGNLPWFTQPDGLRNHFSKFGTIVS 720
VRYSVDMNPGTRRNPEVLNSTPKKILMYESQHKVYVGNLPWFTQPDGLRNHFSKFGTIVS
Sbjct: 181 VRYSVDMNPGTRRNPEVLNSTPKKILMYESQHKVYVGNLPWFTQPDGLRNHFSKFGTIVS 240
Query: 721 TRVLHDRKTGRNRVFAFLSFTSGEERDAALSFNGTQYEGRRIIVREGIEKSES 879
TRVLHDRKTGRNRVFAFLSFTSGEERDAALSFNGTQYEGRRIIVREGIEKSES
Sbjct: 241 TRVLHDRKTGRNRVFAFLSFTSGEERDAALSFNGTQYEGRRIIVREGIEKSES 293
>gb|AAF26472.1|AC007323_13 T25K16.8 [Arabidopsis thaliana]
Length = 679
Score = 368 bits (945), Expect = e-101
Identities = 191/226 (84%), Positives = 192/226 (84%), Gaps = 10/226 (4%)
Frame = +1
Query: 178 AQPLQIXXXXXXXXXXXXXXXXXXXXXXXXPNKDSVVSKAEPVKKPRPCELYVCNIPRSY 357
+QPLQI PNKDSVVSKAEPVKKPRPCELYVCNIPRSY
Sbjct: 416 SQPLQIKVSSSELSVLDEEKEEEVVKGEAEPNKDSVVSKAEPVKKPRPCELYVCNIPRSY 475
Query: 358 DIAQLLDMFQPFGTVISVEV-SRNPQTGESRGSGYVTMGSINSAKIAIASLDGT------ 516
DIAQLLDMFQPFGTVISVEV SRNPQTGESRGSGYVTMGSINSAKIAIASLDGT
Sbjct: 476 DIAQLLDMFQPFGTVISVEVVSRNPQTGESRGSGYVTMGSINSAKIAIASLDGTVRARET 535
Query: 517 ---EVGGREMRVRYSVDMNPGTRRNPEVLNSTPKKILMYESQHKVYVGNLPWFTQPDGLR 687
EVGGREMRVRYSVDMNPGTRRNPEVLNSTPKKILMYESQHKVYVGNLPWFTQPDGLR
Sbjct: 536 KKQEVGGREMRVRYSVDMNPGTRRNPEVLNSTPKKILMYESQHKVYVGNLPWFTQPDGLR 595
Query: 688 NHFSKFGTIVSTRVLHDRKTGRNRVFAFLSFTSGEERDAALSFNGT 825
NHFSKFGTIVSTRVLHDRKTGRNRVFAFLSFTSGEERDAALSFNGT
Sbjct: 596 NHFSKFGTIVSTRVLHDRKTGRNRVFAFLSFTSGEERDAALSFNGT 641
>pir||S49463 RNA-binding protein RNP1 precursor - kidney
bean|gi|558629|emb|CAA57551.1| chloroplast RNA binding
protein [Phaseolus vulgaris]
Length = 287
Score = 117 bits (292), Expect = 3e-25
Identities = 71/198 (35%), Positives = 111/198 (55%), Gaps = 3/198 (1%)
Frame = +1
Query: 277 DSVVSKAEPVKKPRPCELYVCNIPRSYDIAQLLDMFQPFGTVISVEVSRNPQTGESRGSG 456
+ VV++ + + +LY N+P S D A+L + Q +G+ +EV + TG+SRG
Sbjct: 98 EEVVAEQDSDESFASTKLYFGNLPYSVDSAKLAGLIQDYGSAELIEVLYDRDTGKSRGFA 157
Query: 457 YVTMGSINSAKIAIASLDGTEVGGREMRVRYSVDMNPGTRRNPEVLNSTPKKILMYESQH 636
+VTM I I +LDG E GR +RV +S P+ K+ L E++H
Sbjct: 158 FVTMSCIEDCNAVIENLDGKEYLGRTLRVNFS--------NKPKA-----KEPLYPETEH 204
Query: 637 KVYVGNLPWFTQPDGLRNHFSKFGTIVSTRVLHDRKTGRNRVFAFLSFTSGEERDAAL-S 813
K++VGNL W + L F ++GT+V RVL+D +TGR+R + F+ F++ EE +AAL +
Sbjct: 205 KLFVGNLSWSVTNEILTQAFQEYGTVVGARVLYDGETGRSRGYGFVCFSTKEEMEAALGA 264
Query: 814 FNGTQYEGR--RIIVREG 861
N + EGR R+ + EG
Sbjct: 265 LNDVELEGRAMRVSLAEG 282
>sp|P19684|ROC5_NICSY 33 kDa ribonucleoprotein, chloroplast
precursor|gi|100390|pir||S12111 ribonucleoprotein, 33K,
precursor - common tobacco|gi|20005|emb|CAA37879.1| 33
kDa ribonucleoprotein precursor [Nicotiana tabacum]
Length = 324
Score = 115 bits (289), Expect = 6e-25
Identities = 68/174 (39%), Positives = 103/174 (59%), Gaps = 3/174 (1%)
Frame = +1
Query: 328 LYVCNIPRSYDIAQLLDMFQPFGTVISVEVSRNPQTGESRGSGYVTMGSINSAKIAIASL 507
LYV N+P S +QL ++F GTV +VE+ + T SRG +VTMGS+ AK AI
Sbjct: 116 LYVGNLPFSMTSSQLSEIFAEAGTVANVEIVYDRVTDRSRGFAFVTMGSVEEAKEAIRLF 175
Query: 508 DGTEVGGREMRVRYSVDMNPGTRRNPEVLNSTPKKIL--MYESQHKVYVGNLPWFTQPDG 681
DG++VGGR ++V + G R EV+++ + +S HK+YV NL W G
Sbjct: 176 DGSQVGGRTVKVNFPEVPRGGER---EVMSAKIRSTYQGFVDSPHKLYVANLSWALTSQG 232
Query: 682 LRNHFSKFGTIVSTRVLHDRKTGRNRVFAFLSFTSGEERDAAL-SFNGTQYEGR 840
LR+ F+ +S +V++DR +GR+R F F++F+S E ++AL + N + EGR
Sbjct: 233 LRDAFADQPGFMSAKVIYDRSSGRSRGFGFITFSSAEAMNSALDTMNEVELEGR 286
Score = 53.5 bits (127), Expect = 4e-06
Identities = 28/73 (38%), Positives = 46/73 (62%), Gaps = 1/73 (1%)
Frame = +1
Query: 637 KVYVGNLPWFTQPDGLRNHFSKFGTIVSTRVLHDRKTGRNRVFAFLSFTSGEE-RDAALS 813
++YVGNLP+ L F++ GT+ + +++DR T R+R FAF++ S EE ++A
Sbjct: 115 RLYVGNLPFSMTSSQLSEIFAEAGTVANVEIVYDRVTDRSRGFAFVTMGSVEEAKEAIRL 174
Query: 814 FNGTQYEGRRIIV 852
F+G+Q GR + V
Sbjct: 175 FDGSQVGGRTVKV 187
Score = 43.1 bits (100), Expect = 0.005
Identities = 26/97 (26%), Positives = 50/97 (50%)
Frame = +1
Query: 319 PCELYVCNIPRSYDIAQLLDMFQPFGTVISVEVSRNPQTGESRGSGYVTMGSINSAKIAI 498
P +LYV N+ + L D F +S +V + +G SRG G++T S + A+
Sbjct: 216 PHKLYVANLSWALTSQGLRDAFADQPGFMSAKVIYDRSSGRSRGFGFITFSSAEAMNSAL 275
Query: 499 ASLDGTEVGGREMRVRYSVDMNPGTRRNPEVLNSTPK 609
+++ E+ GR +R+ + P + +P V+ ++P+
Sbjct: 276 DTMNEVELEGRPLRLNVAGQKAPVS--SPPVVETSPE 310
>pir||S77714 RNA-binding protein precursor, 33K - wood
tobacco|gi|311952|emb|CAA41253.1| 33 kd chloroplast
ribonucleoprotein [Nicotiana sylvestris]
Length = 319
Score = 115 bits (287), Expect = 1e-24
Identities = 68/174 (39%), Positives = 102/174 (58%), Gaps = 3/174 (1%)
Frame = +1
Query: 328 LYVCNIPRSYDIAQLLDMFQPFGTVISVEVSRNPQTGESRGSGYVTMGSINSAKIAIASL 507
LYV N+P S +QL ++F GTV +VE+ + T SRG +VTMGS+ AK AI
Sbjct: 111 LYVGNLPFSMTSSQLSEIFAEAGTVANVEIVYDRVTDRSRGFAFVTMGSVEEAKEAIRLF 170
Query: 508 DGTEVGGREMRVRYSVDMNPGTRRNPEVLNSTPKKIL--MYESQHKVYVGNLPWFTQPDG 681
DG++VGGR ++V + G R EV+++ + +S HK+YV NL W G
Sbjct: 171 DGSQVGGRTVKVNFPEVPRGGER---EVMSAKIRSTYQGFVDSPHKLYVANLSWALTSQG 227
Query: 682 LRNHFSKFGTIVSTRVLHDRKTGRNRVFAFLSFTSGEERDAAL-SFNGTQYEGR 840
LR+ F+ +S +V++DR +GR+R F F++F+S E +AL + N + EGR
Sbjct: 228 LRDAFADQPGFMSAKVIYDRSSGRSRGFGFITFSSAEAMKSALDTMNEVELEGR 281
Score = 53.5 bits (127), Expect = 4e-06
Identities = 28/73 (38%), Positives = 46/73 (62%), Gaps = 1/73 (1%)
Frame = +1
Query: 637 KVYVGNLPWFTQPDGLRNHFSKFGTIVSTRVLHDRKTGRNRVFAFLSFTSGEE-RDAALS 813
++YVGNLP+ L F++ GT+ + +++DR T R+R FAF++ S EE ++A
Sbjct: 110 RLYVGNLPFSMTSSQLSEIFAEAGTVANVEIVYDRVTDRSRGFAFVTMGSVEEAKEAIRL 169
Query: 814 FNGTQYEGRRIIV 852
F+G+Q GR + V
Sbjct: 170 FDGSQVGGRTVKV 182
Score = 45.1 bits (105), Expect = 0.001
Identities = 27/97 (27%), Positives = 50/97 (50%)
Frame = +1
Query: 319 PCELYVCNIPRSYDIAQLLDMFQPFGTVISVEVSRNPQTGESRGSGYVTMGSINSAKIAI 498
P +LYV N+ + L D F +S +V + +G SRG G++T S + K A+
Sbjct: 211 PHKLYVANLSWALTSQGLRDAFADQPGFMSAKVIYDRSSGRSRGFGFITFSSAEAMKSAL 270
Query: 499 ASLDGTEVGGREMRVRYSVDMNPGTRRNPEVLNSTPK 609
+++ E+ GR +R+ + P +P V+ ++P+
Sbjct: 271 DTMNEVELEGRPLRLNVAGQKAP--LSSPSVVETSPE 305
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 122
effective length of database: 265,824,929
effective search space used: 45456062859
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)