BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.sd.21.C1
(1579 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_171635.1| cytochrome p450 family; protein id: At1g01... 882 0.0
pir||JC7172 cytochrome P450 CYP703A1 - garden petunia|gi|73... 690 0.0
gb|AAL73540.1|AF466200_19 cytochrome P450 protein [Sorghum ... 676 0.0
pir||T52256 cytochrome P-450LXXIA1 [similarity] - avocado|g... 301 1e-84
gb|AAK62346.1| elicitor-inducible cytochrome P450 [Nicotian... 303 1e-83
>ref|NP_171635.1| cytochrome p450 family; protein id: At1g01280.1 [Arabidopsis
thaliana]|gi|25282580|pir||A86143 probable cytochrome
P450 [imported] - Arabidopsis
thaliana|gi|9665139|gb|AAF97323.1|AC023628_4 Putative
cytochrome P450 [Arabidopsis thaliana]
Length = 510
Score = 882 bits (2278), Expect(2) = 0.0
Identities = 433/441 (98%), Positives = 434/441 (98%)
Frame = +2
Query: 212 LENVDAITTNDPDTIREIXFXGRTDVF*SRPKTLAAVHLAYGCGDVALAPMGPHWKRMRR 391
L NVDAITTNDPDTIREI + DVF SRPKTLAAVHLAYGCGDVALAPMGPHWKRMRR
Sbjct: 71 LGNVDAITTNDPDTIREILLR-QDDVFSSRPKTLAAVHLAYGCGDVALAPMGPHWKRMRR 129
Query: 392 ICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLG 571
ICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLG
Sbjct: 130 ICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLG 189
Query: 572 KQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRV 751
KQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRV
Sbjct: 190 KQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRV 249
Query: 752 DEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSLPGENGKAHMEDVEIKALIQDMIAAAT 931
DEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSLPGENGKAHMEDVEIKALIQDMIAAAT
Sbjct: 250 DEFHTKIIDEHRRAKLEDEDKNGDMDFVDVLLSLPGENGKAHMEDVEIKALIQDMIAAAT 309
Query: 932 DTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMH 1111
DTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMH
Sbjct: 310 DTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMH 369
Query: 1112 PAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSG 1291
PAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSG
Sbjct: 370 PAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSG 429
Query: 1292 RVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWSSPGNIDTVEVYGMT 1471
RVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWSSPGNIDTVEVYGMT
Sbjct: 430 RVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWSSPGNIDTVEVYGMT 489
Query: 1472 MPKAKPLRAIAKPRLAAHLYT 1534
MPKAKPLRAIAKPRLAAHLYT
Sbjct: 490 MPKAKPLRAIAKPRLAAHLYT 510
Score = 108 bits (269), Expect(2) = 0.0
Identities = 56/72 (77%), Positives = 56/72 (77%)
Frame = +1
Query: 1 MILVLASLFAVLILNVLLWRWLKASACKAQXXXXXXXXXXXXXXXXQLGPLPHRDLASLC 180
MILVLASLFAVLILNVLLWRWLKASACKAQ QLGPLPHRDLASLC
Sbjct: 1 MILVLASLFAVLILNVLLWRWLKASACKAQRLPPGPPRLPILGNLLQLGPLPHRDLASLC 60
Query: 181 DKYGPLVYLRLG 216
DKYGPLVYLRLG
Sbjct: 61 DKYGPLVYLRLG 72
>pir||JC7172 cytochrome P450 CYP703A1 - garden petunia|gi|7339658|dbj|BAA92894.1|
cytochrome P450 [Petunia x hybrida]
Length = 539
Score = 690 bits (1781), Expect(2) = 0.0
Identities = 342/451 (75%), Positives = 385/451 (84%), Gaps = 11/451 (2%)
Frame = +2
Query: 212 LENVDAITTNDPDTIREIXFXGRTDVF*SRPKTLAAVHLAYGCGDVALAPMGPHWKRMRR 391
L NVDAITTNDP+ IREI + D+F SRP+TLAA+HLAYGCGDVALAP+GP WKRMRR
Sbjct: 90 LGNVDAITTNDPEIIREILVQ-QDDIFASRPRTLAAIHLAYGCGDVALAPLGPKWKRMRR 148
Query: 392 ICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLG 571
ICMEHLLTTKRLESF RA+EA+ L+ DV+ +++ G+ +NL+++LGAFSMNNVTRMLLG
Sbjct: 149 ICMEHLLTTKRLESFGKHRADEAQSLVEDVWAKTQKGETVNLRDLLGAFSMNNVTRMLLG 208
Query: 572 KQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRV 751
KQFFG S P+EA EF+HITH+LFWLLGVIYLGDYLP WRW+DP GCEK+MR+VEKRV
Sbjct: 209 KQFFGAES-AGPQEAMEFMHITHELFWLLGVIYLGDYLPLWRWIDPHGCEKKMREVEKRV 267
Query: 752 DEFHTKIIDEHRR-AKLEDEDKNGDMDFVDVLLSLPGE-----NGKAHMEDVEIKALIQD 913
D+FH +II+EHR+ K DE G+MDFVDVLLSLPGE NGK HM+D EIKALIQD
Sbjct: 268 DDFHMRIIEEHRKNGKNVDE---GEMDFVDVLLSLPGEDEGDGNGKQHMDDTEIKALIQD 324
Query: 914 MIAAATDTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNYLRCVVR 1093
MIAAATDTSAVTNEW MAE IK P V++KIQEELD VVGS+RMV ESDLVHL YLRCVVR
Sbjct: 325 MIAAATDTSAVTNEWPMAEVIKHPNVLKKIQEELDIVVGSDRMVTESDLVHLKYLRCVVR 384
Query: 1094 ETFRMHPAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPERHW 1273
ETFRMHPAGPFLIPHES+R T INGYYIPAKTRVFINTHGLGRNTKIWD++++FRPERH
Sbjct: 385 ETFRMHPAGPFLIPHESIRDTKINGYYIPAKTRVFINTHGLGRNTKIWDNIDEFRPERHL 444
Query: 1274 PVEGSGRVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWSSPG----- 1438
P + RVEISHG DFKILPFSAGKRKCPGAPLGV +VLMALARLFHCFEWS P
Sbjct: 445 PADELSRVEISHGADFKILPFSAGKRKCPGAPLGVKLVLMALARLFHCFEWSPPDGLRPE 504
Query: 1439 NIDTVEVYGMTMPKAKPLRAIAKPRLAAHLY 1531
+IDT+EVYGMTMPKAKPL AIA+PRL HLY
Sbjct: 505 DIDTIEVYGMTMPKAKPLMAIARPRLPDHLY 535
Score = 53.9 bits (128), Expect(2) = 0.0
Identities = 23/26 (88%), Positives = 24/26 (91%)
Frame = +1
Query: 139 QLGPLPHRDLASLCDKYGPLVYLRLG 216
QLG LPHRD+AS CDKYGPLVYLRLG
Sbjct: 66 QLGQLPHRDMASFCDKYGPLVYLRLG 91
>gb|AAL73540.1|AF466200_19 cytochrome P450 protein [Sorghum bicolor]
Length = 526
Score = 676 bits (1743), Expect(2) = 0.0
Identities = 330/454 (72%), Positives = 381/454 (83%), Gaps = 14/454 (3%)
Frame = +2
Query: 212 LENVDAITTNDPDTIREIXFXGRTDVF*SRPKTLAAVHLAYGCGDVALAPMGPHWKRMRR 391
L +DAITT+DP+ IREI + +VF SRP+TLAAVHLAYGCGDVALAP+GP+WKRMRR
Sbjct: 69 LGTIDAITTDDPEVIREILIR-QDEVFASRPRTLAAVHLAYGCGDVALAPLGPNWKRMRR 127
Query: 392 ICMEHLLTTKRLESFTTQRAEEARYLIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLG 571
+CMEHLLTTKRLESF RA+EA +L + V+ +S +GKP+NL+EVLGAFSMNNVTRMLLG
Sbjct: 128 VCMEHLLTTKRLESFAAHRAQEAEHLCQFVWAKSHSGKPVNLREVLGAFSMNNVTRMLLG 187
Query: 572 KQFFGPGSLVSPKEAQEFLHITHKLFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRV 751
KQ+FG S P EA EF+HITH+LF+LLG+IYLGDYLP WRWVDP GCEK+MRDVEK+V
Sbjct: 188 KQYFGIQS-AGPGEAMEFMHITHELFFLLGLIYLGDYLPAWRWVDPYGCEKKMRDVEKKV 246
Query: 752 DEFHTKIIDEHRRAK---------LEDEDKNGDMDFVDVLLSLPGENGKAHMEDVEIKAL 904
D+FH KIIDEHRRA+ L+D+D DMDFVDVLLSLPGENGK HM+D+EIKAL
Sbjct: 247 DDFHQKIIDEHRRAREAKKTRRSSLDDDDGKEDMDFVDVLLSLPGENGKEHMDDMEIKAL 306
Query: 905 IQDMIAAATDTSAVTNEWAMAEAIKQPRVMRKIQEELDNVVGSNRMVDESDLVHLNYLRC 1084
+QDMIAAATDTS+VTNEW MAE IK PRV+R++QEELD V+G +RMV ESDL HL YLRC
Sbjct: 307 MQDMIAAATDTSSVTNEWVMAEVIKNPRVLRRVQEELDAVIGRDRMVAESDLTHLPYLRC 366
Query: 1085 VVRETFRMHPAGPFLIPHESVRATTINGYYIPAKTRVFINTHGLGRNTKIWDDVEDFRPE 1264
VVRE+FRMHPAGPFLIPHES++ TTI GY++PA+TRVFINTH LGRN ++WDDV+ FRPE
Sbjct: 367 VVRESFRMHPAGPFLIPHESLKPTTIMGYHVPARTRVFINTHALGRNPRVWDDVDAFRPE 426
Query: 1265 RHWPVEGSGRVEISHGPDFKILPFSAGKRKCPGAPLGVTMVLMALARLFHCFEWSSPG-- 1438
RH P E RVEISH PDFKILPFSAGKRKCPGAPLGV +VLMALARLFHCF+WS P
Sbjct: 427 RHLPAEEGARVEISHLPDFKILPFSAGKRKCPGAPLGVALVLMALARLFHCFDWSPPDGL 486
Query: 1439 ---NIDTVEVYGMTMPKAKPLRAIAKPRLAAHLY 1531
++DT EVYGMTMPKA PL A+A PRL HLY
Sbjct: 487 RPEDVDTQEVYGMTMPKATPLVAVATPRLPPHLY 520
Score = 48.1 bits (113), Expect(2) = 0.0
Identities = 20/26 (76%), Positives = 21/26 (79%)
Frame = +1
Query: 139 QLGPLPHRDLASLCDKYGPLVYLRLG 216
QL PLPH+D A C KYGPLVYLRLG
Sbjct: 45 QLSPLPHKDFARFCTKYGPLVYLRLG 70
>pir||T52256 cytochrome P-450LXXIA1 [similarity] -
avocado|gi|166949|gb|AAA32913.1| cytochrome P-450LXXIA1
(cyp71A1)
Length = 502
Score = 301 bits (772), Expect(2) = 1e-84
Identities = 164/419 (39%), Positives = 244/419 (58%), Gaps = 8/419 (1%)
Frame = +2
Query: 287 VF*SRPKTLAAVHLAYGCGDVALAPMGPHWKRMRRICMEHLLTTKRLESFTTQRAEEARY 466
+F SRP T AA + Y C DVA +P G +W+++R+IC+ LL+ KR+ S+ + R EE
Sbjct: 94 IFASRPSTTAARRIFYDCTDVAFSPYGEYWRQVRKICVLELLSIKRVNSYRSIREEEVGL 153
Query: 467 LIRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLGKQFFGPGSLVSPKEAQEFLHITHKL 646
++ + + TG+ +NL E+L S +TR+ GK++ G + +F + +L
Sbjct: 154 MMERISQSCSTGEAVNLSELLLLLSSGTITRVAFGKKYEG-----EEERKNKFADLATEL 208
Query: 647 FWLLGVIYLGDYLPFWRWVDP-SGCEKEMRDVEKRVDEFHTKIIDEHRRA-KLEDEDKNG 820
L+G ++GDY P + WVD +G + ++ +D F +ID+H + K D
Sbjct: 209 TTLMGAFFVGDYFPSFAWVDVLTGMDARLKRNHGELDAFVDHVIDDHLLSRKANGSDGVE 268
Query: 821 DMDFVDVLLSLPGENGKA-HMEDVEIKALIQDMIAAATDTSAVTNEWAMAEAIKQPRVMR 997
D VDVLL L ++ H+ +KA+I DM + TDT+AVT EWAMAE IK P VM
Sbjct: 269 QKDLVDVLLHLQKDSSLGVHLNRNNLKAVILDMFSGGTDTTAVTLEWAMAELIKHPDVME 328
Query: 998 KIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMHPAGPFLIPHESVRATTINGYYI 1177
K Q+E+ VVG V+E DL L+YL+ +++ET R+HP P L+P ES R I GY+I
Sbjct: 329 KAQQEVRRVVGKKAKVEEEDLHQLHYLKLIIKETLRLHPVAPLLVPRESTRDVVIRGYHI 388
Query: 1178 PAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSGRVEISHGPDFKILPFSAGKRKC 1357
PAKTRVFIN +GR+ K W++ E+F PER V S + G DF+++PF AG+R C
Sbjct: 389 PAKTRVFINAWAIGRDPKSWENAEEFLPERF--VNNSVDFK---GQDFQLIPFGAGRRGC 443
Query: 1358 PGAPLGVTMVLMALARLFHCFEWSSPG-----NIDTVEVYGMTMPKAKPLRAIAKPRLA 1519
PG G++ V ++LA L + F W PG ++D E G+T+ PL+ +AK L+
Sbjct: 444 PGIAFGISSVEISLANLLYWFNWELPGDLTKEDLDMSEAVGITVHMKFPLQLVAKRHLS 502
Score = 36.2 bits (82), Expect(2) = 1e-84
Identities = 16/26 (61%), Positives = 19/26 (72%)
Frame = +1
Query: 139 QLGPLPHRDLASLCDKYGPLVYLRLG 216
QLG LPHR L SL ++ GPL+ L LG
Sbjct: 46 QLGNLPHRSLRSLANELGPLILLHLG 71
>gb|AAK62346.1| elicitor-inducible cytochrome P450 [Nicotiana tabacum]
Length = 509
Score = 303 bits (777), Expect(2) = 1e-83
Identities = 171/425 (40%), Positives = 257/425 (60%), Gaps = 10/425 (2%)
Frame = +2
Query: 290 F*SRPKTLAAVHLAYGCGDVALAPMGPHWKRMRRICMEHLLTTKRLESFTTQRAEEARYL 469
F RPKT A + Y D+ +P GP+W++ RR+C+ L +TKRL+S+ RAEE L
Sbjct: 97 FVGRPKTAAGKYTTYNYSDITWSPYGPYWRQARRMCLMELFSTKRLDSYEYIRAEELHSL 156
Query: 470 IRDVFKRSETGKPINLKEVLGAFSMNNVTRMLLGKQFF--GPGSLVSPKEAQEFLHITHK 643
+ ++ K S GKPI LK+ L S+N ++RM+LGK++ S+V+P+E ++ L +
Sbjct: 157 LHNLNKIS--GKPIVLKDYLTTLSLNVISRMVLGKRYLDESENSIVTPEEFKKMLD---E 211
Query: 644 LFWLLGVIYLGDYLPFWRWVDPSGCEKEMRDVEKRVDEFHTKIIDEH--RRAKLEDEDKN 817
LF L GV+ +GD +P+ ++D G K M+ V K+ D+F +IDEH RR +E+
Sbjct: 212 LFLLNGVLNIGDSIPWIDFMDLQGYVKRMKFVSKKFDKFLEHVIDEHNVRRNGVENYIAK 271
Query: 818 GDMDFVDVLLSLPGENG-KAHMEDVEIKALIQDMIAAATDTSAVTNEWAMAEAIKQPRVM 994
D VDVLL L + + +E +KA QDM+A T++SAVT EWA++E +K+P +
Sbjct: 272 ---DMVDVLLQLADDPTLEVKLERHGVKAFTQDMLAGGTESSAVTVEWAISELLKKPEIF 328
Query: 995 RKIQEELDNVVGSNRMVDESDLVHLNYLRCVVRETFRMHPAGPFLIPHESVRATTINGYY 1174
+K EELD V+G NR V E D+ +L Y+ +V+ET R+HP P L+P E + GY
Sbjct: 329 KKATEELDRVIGQNRWVQEKDIPNLPYIEAIVKETMRLHPVAPMLVPRECREDCKVAGYD 388
Query: 1175 IPAKTRVFINTHGLGRNTKIWDDVEDFRPERHWPVEGSGRVEISHGPDFKILPFSAGKRK 1354
+ TRV ++ +GR+ +WD+ E F+PER E S V+ G DF++LPF AG+R
Sbjct: 389 VKKGTRVLVSVWTIGRDPTLWDEPEAFKPERFH--EKSIDVK---GHDFELLPFGAGRRM 443
Query: 1355 CPGAPLGVTMVLMALARLFHCFEWSSPGN-----IDTVEVYGMTMPKAKPLRAIAKPRLA 1519
CPG LG+ ++ +LA L H F WS P N +D E++G++ PK PL + +PRL+
Sbjct: 444 CPGYNLGLKVIQASLANLIHGFNWSLPDNMTPEDLDMDEIFGLSTPKKFPLATVIEPRLS 503
Query: 1520 AHLYT 1534
LY+
Sbjct: 504 PKLYS 508
Score = 30.4 bits (67), Expect(2) = 1e-83
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +1
Query: 142 LGPLPHRDLASLCDKYGPLVYLRLG 216
+G LPHR + L KYGP++ L+ G
Sbjct: 49 IGNLPHRSIHELSLKYGPIMQLQFG 73
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 128
effective length of database: 257,790,653
effective search space used: 102342889241
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)