BLASTX 1.4.13-Paracel [2002-12-12]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AU236273.f
         (496 letters)

Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr 
           1,339,046 sequences; 429,188,541 total letters

Searching...................................................done


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|NP_171626.1|  hypothetical protein; protein id: At1g0118...   215   5e-66
gb|ZP_00023201.1|  hypothetical protein [Ralstonia metallidu...    33   2.2  
gb|ZP_00074575.1|  hypothetical protein [Trichodesmium eryth...    31   8.4  
ref|NP_116674.1|  May regulate vacuole homeostasis; mutation...    31   8.4  
sp|P34756|FAB1_YEAST  1-phosphatidylinositol-3-phosphate 5-k...    31   8.4  

>ref|NP_171626.1| hypothetical protein; protein id: At1g01180.1 [Arabidopsis
           thaliana]|gi|25406717|pir||A86142 protein T25K16.17
           [imported] - Arabidopsis
           thaliana|gi|6715637|gb|AAF26464.1|AC007323_5 T25K16.17
           [Arabidopsis thaliana]
          Length = 478

 Score =  215 bits (548), Expect(2) = 5e-66
 Identities = 104/115 (90%), Positives = 105/115 (90%)
 Frame = +3

Query: 33  MKEDRRLPHKRDAFQFLKTKAAYVIVIVLTYAFGYFSAYHYHXXXXXXXXXSTTAVETTK 212
           MKEDRRLPHKRDAFQFLKTKAAYVIVIVLTYAFGYFSAYHYH         STTAVETTK
Sbjct: 1   MKEDRRLPHKRDAFQFLKTKAAYVIVIVLTYAFGYFSAYHYHQPLQQQLPPSTTAVETTK 60

Query: 213 PQVCSIDNFRVTTPCGNLVPPELIRQTVIDRIFNGTSPYIDFPPPHAKKFLRPSQ 377
           PQVCSIDNFRVTTPCGNLVPPELIRQTVIDRIFNGTSPYIDFPPPHAKKFLRP +
Sbjct: 61  PQVCSIDNFRVTTPCGNLVPPELIRQTVIDRIFNGTSPYIDFPPPHAKKFLRPKR 115




 Score = 57.0 bits (136), Expect(2) = 5e-66
 Identities = 28/28 (100%), Positives = 28/28 (100%)
 Frame = +1

Query: 370 RVKPKTIVEVGSFLGASAIHMANLTRRL 453
           RVKPKTIVEVGSFLGASAIHMANLTRRL
Sbjct: 132 RVKPKTIVEVGSFLGASAIHMANLTRRL 159



>gb|ZP_00023201.1| hypothetical protein [Ralstonia metallidurans]
          Length = 619

 Score = 32.7 bits (73), Expect = 2.2
 Identities = 15/30 (50%), Positives = 19/30 (63%)
 Frame = +1

Query: 382 KTIVEVGSFLGASAIHMANLTRRLWARGDS 471
           KT+ E    LGA+   +A +TR LWA GDS
Sbjct: 505 KTLGEQARALGAATARLAEVTRTLWAAGDS 534



>gb|ZP_00074575.1| hypothetical protein [Trichodesmium erythraeum IMS101]
          Length = 97

 Score = 30.8 bits (68), Expect = 8.4
 Identities = 12/27 (44%), Positives = 18/27 (66%)
 Frame = +1

Query: 373 VKPKTIVEVGSFLGASAIHMANLTRRL 453
           VKPK I++VG + G S IHM  + + +
Sbjct: 9   VKPKVIIKVGVWKGQSTIHMTEIAKEI 35



>ref|NP_116674.1| May regulate vacuole homeostasis; mutation causes pleiotropic effects
            on nuclear migration and orientation and separation of
            mitotic chromosomes; Fab1p [Saccharomyces
            cerevisiae]|gi|1084543|pir||S56274 FAB1 protein - yeast
            (Saccharomyces cerevisiae)|gi|836774|dbj|BAA09258.1| FAB1
            protein [Saccharomyces cerevisiae]
          Length = 2278

 Score = 30.8 bits (68), Expect = 8.4
 Identities = 19/64 (29%), Positives = 32/64 (49%)
 Frame = +3

Query: 189  TTAVETTKPQVCSIDNFRVTTPCGNLVPPELIRQTVIDRIFNGTSPYIDFPPPHAKKFLR 368
            + A E  K  +  +D F   +PC   +P  L+ +++ D+  N  S +  F    AK +L 
Sbjct: 1427 SNATEEQKLMLQDLDTFYADSPCDQHLPLNLVIKSLYDKAVNWNSTFAIF----AKSYL- 1481

Query: 369  PSQT 380
            PS+T
Sbjct: 1482 PSET 1485



>sp|P34756|FAB1_YEAST 1-phosphatidylinositol-3-phosphate 5-kinase FAB1
            (Phosphatidylinositol-3-phosphate 5-kinase) (Type III PIP
            kinase)|gi|398498|gb|AAA81360.1| Fab1p
          Length = 2278

 Score = 30.8 bits (68), Expect = 8.4
 Identities = 19/64 (29%), Positives = 32/64 (49%)
 Frame = +3

Query: 189  TTAVETTKPQVCSIDNFRVTTPCGNLVPPELIRQTVIDRIFNGTSPYIDFPPPHAKKFLR 368
            + A E  K  +  +D F   +PC   +P  L+ +++ D+  N  S +  F    AK +L 
Sbjct: 1427 SNATEEQKLMLQDLDTFYADSPCDQHLPLNLVIKSLYDKAVNWNSTFAIF----AKSYL- 1481

Query: 369  PSQT 380
            PS+T
Sbjct: 1482 PSET 1485



  Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
    Posted date:  Feb 17, 2003 10:02 AM
  Number of letters in database: 429,188,541
  Number of sequences in database:  1,339,046
  
Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 113
effective length of database: 277,876,343
effective search space used: 14171693493
frameshift window, decay const: 50,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)