BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.CL.3.cl.359.Contig1
(2432 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAO00820.1| Unknown protein [Arabidopsis thaliana] 1071 0.0
ref|NP_015076.1| Positive regulator of GAL genes; Gal4p [Sa... 243 1e-62
sp|P08657|LAC9_KLULA Lactose regulatory protein LAC9|gi|173... 167 9e-40
pir||A25762 regulatory protein LAC9 - yeast (Kluyveromyces ... 165 3e-39
ref|NP_595318.1| putative transcriptional activator with fu... 93 2e-17
>gb|AAO00820.1| Unknown protein [Arabidopsis thaliana]
Length = 549
Score = 1071 bits (2769), Expect = 0.0
Identities = 522/549 (95%), Positives = 522/549 (95%)
Frame = +3
Query: 582 MAALSIKPEGAGYFGASSSVVPLRALFDHGFDLNMPVRSARSGGVPLKAQLLESAPSGLI 761
MAALSIKPEGAGYFGASSSVVPLRALFDHGFDLNMPVRSARSGGVPLKAQLLESAPSGLI
Sbjct: 1 MAALSIKPEGAGYFGASSSVVPLRALFDHGFDLNMPVRSARSGGVPLKAQLLESAPSGLI 60
Query: 762 EQAFIDAFFLNYHTSYPFVHEPTFRAQFNDPSIRPHGTAWHILLNTILALGAWCIGDDSS 941
EQAFIDAFFLNYHTSYPFVHEPTFRAQFNDPSIRPHGTAWHILLNTILALGAWCIGDDSS
Sbjct: 61 EQAFIDAFFLNYHTSYPFVHEPTFRAQFNDPSIRPHGTAWHILLNTILALGAWCIGDDSS 120
Query: 942 DLDITFYQEARGYLQQASVFETGNXXXXXXXXXXSNYAQKRNKPNTGWNYLGLAVRMAMS 1121
DLDITFYQEARGYLQQASVFETGN SNYAQKRNKPNTGWNYLGLAVRMAMS
Sbjct: 121 DLDITFYQEARGYLQQASVFETGNLTLVQGLLLLSNYAQKRNKPNTGWNYLGLAVRMAMS 180
Query: 1122 LGLHKEFPGWKISLLQREIRRRLWWGVFIFDSGAAKTFGRPILLPEESVMDAKQVRNIHE 1301
LGLHKEFPGWKISLLQREIRRRLWWGVFIFDSGAAKTFGRPILLPEESVMDAKQVRNIHE
Sbjct: 181 LGLHKEFPGWKISLLQREIRRRLWWGVFIFDSGAAKTFGRPILLPEESVMDAKQVRNIHE 240
Query: 1302 DELTPTTTALPDESLGPTIYSGLIAQARFHILTNSVYQRLISSPSLTPEDTLKLQQPIEE 1481
DELTPTTTALPDESLGPTIYSGLIAQARFHILTNSVYQRLISSPSLTPEDTLKLQQPIEE
Sbjct: 241 DELTPTTTALPDESLGPTIYSGLIAQARFHILTNSVYQRLISSPSLTPEDTLKLQQPIEE 300
Query: 1482 WYNGLPSYLQHPQMPLSVQPTNPDPDSLALVRNRLLWRNWNLTILIHRPILLRWASRRWA 1661
WYNGLPSYLQHPQMPLSVQPTNPDPDSLALVRNRLLWRNWNLTILIHRPILLRWASRRWA
Sbjct: 301 WYNGLPSYLQHPQMPLSVQPTNPDPDSLALVRNRLLWRNWNLTILIHRPILLRWASRRWA 360
Query: 1662 PLSGMPGTPDAGTEDPCETECRVRCLQNARLTISSISEYMDNYICTRLGAWYMLYFLFQA 1841
PLSGMPGTPDAGTEDPCETECRVRCLQNARLTISSISEYMDNYICTRLGAWYMLYFLFQA
Sbjct: 361 PLSGMPGTPDAGTEDPCETECRVRCLQNARLTISSISEYMDNYICTRLGAWYMLYFLFQA 420
Query: 1842 GLIPIVFFMTDPSNPEAATWLQDIETTKALLTHPSLGTNRFATRCYEVINRLLGPPIPPT 2021
GLIPIVFFMTDPSNPEAATWLQDIETTKALLTHPSLGTNRFATRCYEVINRLLGPPIPPT
Sbjct: 421 GLIPIVFFMTDPSNPEAATWLQDIETTKALLTHPSLGTNRFATRCYEVINRLLGPPIPPT 480
Query: 2022 QVIPTANPHEAXXXXXXXXXXXXXXXXXFPEQLFSDPGFGGSLFPVEQQMQMNAGGMDFS 2201
QVIPTANPHEA FPEQLFSDPGFGGSLFPVEQQMQMNAGGMDFS
Sbjct: 481 QVIPTANPHEAQQQPMHHIPQQPQNMMPFPEQLFSDPGFGGSLFPVEQQMQMNAGGMDFS 540
Query: 2202 EWVNFPPTE 2228
EWVNFPPTE
Sbjct: 541 EWVNFPPTE 549
>ref|NP_015076.1| Positive regulator of GAL genes; Gal4p [Saccharomyces
cerevisiae]|gi|1169823|sp|P04386|GAL4_YEAST Regulatory
protein GAL4|gi|73246|pir||RGBYG4 regulatory protein GAL4
- yeast (Saccharomyces
cerevisiae)|gi|171558|gb|AAA34626.1| GAL4
protein|gi|1061241|emb|CAA91596.1| GAL4 [Saccharomyces
cerevisiae]|gi|1370511|emb|CAA97969.1| ORF YPL248c
[Saccharomyces cerevisiae]|gi|4995952|dbj|BAA78208.1|
Gal4 [Drosophila melanogaster]
Length = 881
Score = 243 bits (620), Expect = 1e-62
Identities = 188/677 (27%), Positives = 281/677 (40%), Gaps = 56/677 (8%)
Frame = +3
Query: 186 CDECRLRKSRCSKERPVCLQCRQLNKECLYSPKVSRSPLTRQHLTYVEDRLHSFETALGR 365
CD CRL+K +CSKE+P C +C + N EC YSPK RSPLTR HLT VE RL E
Sbjct: 11 CDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESRLERLEQLFLL 70
Query: 366 LFPGG-----------------------------------------DLDATLR----SLL 410
+FP D+ TLR S
Sbjct: 71 IFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPLTLRQHRISAT 130
Query: 411 QNPEXXXXXXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXDGFDWAEKEITLGDLTDGMAA 590
+ E H GFDW+E++ D++DG+
Sbjct: 131 SSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDFMPRDALHGFDWSEED----DMSDGLPF 186
Query: 591 LSIKPEGAGYFGASSSVVPLRALFDHGFDLNMPVRSARSGGVPLKAQLLE--SAPSGLIE 764
L P G+FG S + LR++ GF P S L + + + S
Sbjct: 187 LKTDPNNNGFFGDGSLLCILRSI---GFK---PENYTNSNVNRLPTMITDRYTLASRSTT 240
Query: 765 QAFIDAFFLNYHTSYPFVHEPTFRAQFNDPSIRPHGTAWHILLNTILALGAWCIGDDSSD 944
+ ++ N+H P VH PT +N+ W IL N ILA+GAWCI +S+D
Sbjct: 241 SRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQILFNCILAIGAWCIEGESTD 300
Query: 945 LDITFYQEARGYLQQASVFETGNXXXXXXXXXXSNYAQKRNKPNTGWNYLGLAVRMAMSL 1124
+D+ +YQ A+ +L + VFE+G+ S Y Q R K NT +N+ ++RMA+SL
Sbjct: 301 IDVFYYQNAKSHL-TSKVFESGSIILVTALHLLSRYTQWRQKTNTSYNFHSFSIRMAISL 359
Query: 1125 GLHKEFPGWKISLLQREIRRRLWWGVFIFDSGAAKTFGRPILLPEESVMDAKQVRNIHED 1304
GL+++ P E RRR+WW V+ ++ + +GR I L + ++ V D
Sbjct: 360 GLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRSIQLSQNTISFPSSV-----D 414
Query: 1305 ELTPTTTALPDESLGPTIYSGLIAQARFHILTNSVYQR----LISSPSLTPEDTLKLQQP 1472
++ TTT GPTIY G+I AR + +Y+ + + L +
Sbjct: 415 DVQRTTT-------GPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICAKKCLMICNE 467
Query: 1473 IEEWYNGLPSYLQHPQMPLSVQPTNPDPDSLALVRNRLLWRNWNLTILIHRPILLRWASR 1652
IEE P +LQ ++ + L+ R L W+ +L I + R + +
Sbjct: 468 IEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVLRDFFTNFTQK 527
Query: 1653 RWAPLSGMPGTPDAGTEDPCETE-CRVRCLQNARLTISSISEYMDNYICTRLGAWYMLYF 1829
+ D E + C + A+ T+ S+S YMDN+ T AW Y+
Sbjct: 528 K------SQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAWNCSYY 581
Query: 1830 LFQAGLIPIVFFM----TDPSNPEAATWLQDIETTKALLTHPSLGTNRFATRCYEVINRL 1997
LF A L+PI + ++ N E A LQ I T LL + + + +V+ +
Sbjct: 582 LFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYIQVLEEV 641
Query: 1998 LGPPIPPTQVIPTANPH 2048
P + IP PH
Sbjct: 642 CAPFLLSQCAIPL--PH 656
>sp|P08657|LAC9_KLULA Lactose regulatory protein LAC9|gi|173307|gb|AAA35266.1| LAC9
protein|gi|298015|emb|CAA29565.1| LAC9 regulatory protein
(AA 1 - 865) [Kluyveromyces lactis]
Length = 865
Score = 167 bits (422), Expect = 9e-40
Identities = 126/401 (31%), Positives = 196/401 (48%), Gaps = 6/401 (1%)
Frame = +3
Query: 768 AFIDAFFLNYHTSYPFVHEPTFRAQFNDPSIRPHGTA-WHILLNTILALGAWCIGDDSSD 944
A+IDA+F +YH YP V + F AQ+ND I+P WHILLN +LALG+WC SS
Sbjct: 387 AYIDAYFKHYHALYPLVSKEMFFAQYND-QIKPENVEIWHILLNAVLALGSWCSNSCSSH 445
Query: 945 LDITFYQEARGYLQQASVFETGNXXXXXXXXXXSNYAQKRNKPNTGWNYLGLAVRMAMSL 1124
+ +YQ A YL A V ETG+ ++Y QK +KPNT W+ +GL MA SL
Sbjct: 446 HTL-YYQNALSYLSTA-VLETGSTDLTIALILLTHYVQKMHKPNTAWSLIGLCSHMATSL 503
Query: 1125 GLHKEFPGWKISLLQREIRRRLWWGVFIFDSGAAKTFGRPILLPEESVMDAKQVRNIHED 1304
GLH++ P I +++RR LWW ++ + GRP LLP +D
Sbjct: 504 GLHRDLPNSTIH--DQQLRRVLWWTIYCTGCDLSLETGRPSLLPNLQAIDIP-------- 553
Query: 1305 ELTPTTTALPDESLGPTIYSGLIAQARFHILTNSVYQRLISSPSLTPEDTLKLQQPIEE- 1481
P ++A E P+IYS +I ++++ + + Q+ +S+ S QQ E
Sbjct: 554 --LPASSATIKE---PSIYSSIIQESQW----SQILQQKLSNNS--------YQQSAGEC 596
Query: 1482 --WYNGLPSYLQHPQMPLSVQPTNPDPDSLALVRNRLLWRNWNLTILIHRPILLRWASRR 1655
W++ + ++L H P + + + AL +L W L ++ RP + S
Sbjct: 597 LSWFDSVQAFLDHWPTP------STEAELKALNETQLDW----LPLVKFRPYWMFHCS-- 644
Query: 1656 WAPLSGMPGTPDAGTEDPCETECRVRCLQNARLTISSISEYMDNYICTRLGAWYMLYFLF 1835
L + DA T++ C+ CLQ + I S++ ++ +Y L WY ++L
Sbjct: 645 LISLFSVFFEEDAPTDNNV-IRCKELCLQLSSRNIFSVATFVRSYAFNSLSCWYATHYLV 703
Query: 1836 QAGLIPIVFFMTDPSNPEAATWLQDIETTKA--LLTHPSLG 1952
++ L+P+ F +P+ A W ET KA L H ++G
Sbjct: 704 RSALVPLHF--ASRISPQHALW----ETVKAQLLSAHEAMG 738
Score = 79.3 bits (194), Expect = 3e-13
Identities = 36/76 (47%), Positives = 49/76 (64%)
Frame = +3
Query: 174 LHV*CDECRLRKSRCSKERPVCLQCRQLNKECLYSPKVSRSPLTRQHLTYVEDRLHSFET 353
+H CD CR +K +CSK P C C + N +C+YSP+V R+PLTR HLT +E+R+ E
Sbjct: 91 MHQACDACRKKKWKCSKTVPTCTNCLKYNLDCVYSPQVVRTPLTRAHLTEMENRVAELEQ 150
Query: 354 ALGRLFPGGDLDATLR 401
L LFP D+D L+
Sbjct: 151 FLKELFPVWDIDRLLQ 166
>pir||A25762 regulatory protein LAC9 - yeast (Kluyveromyces marxianus var. lactis)
Length = 865
Score = 165 bits (417), Expect = 3e-39
Identities = 125/401 (31%), Positives = 195/401 (48%), Gaps = 6/401 (1%)
Frame = +3
Query: 768 AFIDAFFLNYHTSYPFVHEPTFRAQFNDPSIRPHGTA-WHILLNTILALGAWCIGDDSSD 944
A+IDA+F +YH YP V + F A +ND I+P WHILLN +LALG+WC SS
Sbjct: 387 AYIDAYFKHYHALYPLVSKEMFFAHYND-QIKPENVEIWHILLNAVLALGSWCSNSCSSH 445
Query: 945 LDITFYQEARGYLQQASVFETGNXXXXXXXXXXSNYAQKRNKPNTGWNYLGLAVRMAMSL 1124
+ +YQ A YL A V ETG+ ++Y QK +KPNT W+ +GL MA SL
Sbjct: 446 HTL-YYQNALSYLSTA-VLETGSTDLTIALILLTHYVQKMHKPNTAWSLIGLCSHMATSL 503
Query: 1125 GLHKEFPGWKISLLQREIRRRLWWGVFIFDSGAAKTFGRPILLPEESVMDAKQVRNIHED 1304
GLH++ P I +++RR LWW ++ + GRP LLP +D
Sbjct: 504 GLHRDLPNSTIH--DQQLRRVLWWTIYCTGCDLSLETGRPSLLPNLQAIDIP-------- 553
Query: 1305 ELTPTTTALPDESLGPTIYSGLIAQARFHILTNSVYQRLISSPSLTPEDTLKLQQPIEE- 1481
P ++A E P+IYS +I ++++ + + Q+ +S+ S QQ E
Sbjct: 554 --LPASSATIKE---PSIYSSIIQESQW----SQILQQKLSNNS--------YQQSAGEC 596
Query: 1482 --WYNGLPSYLQHPQMPLSVQPTNPDPDSLALVRNRLLWRNWNLTILIHRPILLRWASRR 1655
W++ + ++L H P + + + AL +L W L ++ RP + S
Sbjct: 597 LSWFDSVQAFLDHWPTP------STEAELKALNETQLDW----LPLVKFRPYWMFHCS-- 644
Query: 1656 WAPLSGMPGTPDAGTEDPCETECRVRCLQNARLTISSISEYMDNYICTRLGAWYMLYFLF 1835
L + DA T++ C+ CLQ + I S++ ++ +Y L WY ++L
Sbjct: 645 LISLFSVFFEEDAPTDNNV-IRCKELCLQLSSRNIFSVATFVRSYAFNSLSCWYATHYLV 703
Query: 1836 QAGLIPIVFFMTDPSNPEAATWLQDIETTKA--LLTHPSLG 1952
++ L+P+ F +P+ A W ET KA L H ++G
Sbjct: 704 RSALVPLHF--ASRISPQHALW----ETVKAQLLSAHEAMG 738
Score = 79.3 bits (194), Expect = 3e-13
Identities = 36/76 (47%), Positives = 49/76 (64%)
Frame = +3
Query: 174 LHV*CDECRLRKSRCSKERPVCLQCRQLNKECLYSPKVSRSPLTRQHLTYVEDRLHSFET 353
+H CD CR +K +CSK P C C + N +C+YSP+V R+PLTR HLT +E+R+ E
Sbjct: 91 MHQACDACRKKKWKCSKTVPTCTNCLKYNLDCVYSPQVVRTPLTRAHLTEMENRVAELEQ 150
Query: 354 ALGRLFPGGDLDATLR 401
L LFP D+D L+
Sbjct: 151 FLKELFPVWDIDRLLQ 166
>ref|NP_595318.1| putative transcriptional activator with fungal binuclear cluster
domain [Schizosaccharomyces pombe]|gi|7491692|pir||T40521
hypothetical protein SPBC530.05 - fission yeast
(Schizosaccharomyces pombe)|gi|3150252|emb|CAA19171.1|
putative transcriptional activator with fungal binuclear
cluster domain [Schizosaccharomyces pombe]
Length = 743
Score = 93.2 bits (230), Expect = 2e-17
Identities = 105/474 (22%), Positives = 179/474 (37%), Gaps = 32/474 (6%)
Frame = +3
Query: 186 CDECRLRKSRCSKERPVCLQCRQLNKECLYSPKVSRSPLTRQ-HLTYVEDRLHSFETALG 362
CD CR RK RC ++P C C C+++ + R RQ ++ + RL E+ L
Sbjct: 11 CDMCRKRKIRCDGKQPACSNCVSHGIPCVFTARPKRRTGQRQMYIKSLVSRLEQMESTLR 70
Query: 363 RLFPGGDLDATLRSLLQNPEXXXXXXXXXXXXXXXXXXHXXXXXXXXXXXXXXXXDGFDW 542
+ P D + P+ H D
Sbjct: 71 SVIPNYDQQPDIIHPSSTPKNY----------------HDLNCTKGETSSDDSTDD---- 110
Query: 543 AEKEITLGDLTDGMAALSIKPEGAG-YFGASS--SVVPLRALFDHGFDLNMPVRS---AR 704
+ L + M L P G+ YFG+SS S++ A F G + + + A+
Sbjct: 111 ------IAFLNEKMGTLVTTPIGSQKYFGSSSTFSIIQHAAKFASGVESDKVLEHLSMAK 164
Query: 705 SGGVPLKAQLLESA----PSGLIEQAFIDAFFLNYHTSYPFVHEPTFRAQFNDPSI--RP 866
SG + + + + PS I +IDA+F +Y+ +P F +F P+ +P
Sbjct: 165 SGCLFDPDESFDGSKAELPSSEIANIYIDAYFKSYNPLFPVFTRENFYQKFGSPNCFKKP 224
Query: 867 HGTAWHILLN-----TILALGAWCIGDDSSDLDIT--FYQEARGYLQQASVFETGNXXXX 1025
G+ L+N +L+LG I D + ++ G V + +
Sbjct: 225 DGSI--DLVNYASYVVVLSLGCLAIADTEEQVSRANALFKNTLGI--SIEVTKDMSFRTL 280
Query: 1026 XXXXXXSNYAQKRNKPNTGWNYLGLAVRMAMSLGLHKEFPGWKISLLQREIRRRLWWGVF 1205
S Y +KPN W +G+ VR+A +LGLH+ W I E + RL+W ++
Sbjct: 281 VFNFLTSVYYCAVSKPNAVWLNVGVVVRVAQTLGLHRNSAMWSIGKEDAEEKARLFWYIY 340
Query: 1206 IFDSGAAKTFGRPILLPEESVMDAKQVRNIHEDELTPTTTAL------PDESLGPTIYSG 1367
D ++ G+P+ ++ + D++ P + P E ++
Sbjct: 341 YLDRVSSMMTGKPVAFQDDDI-----------DQMVPFYSIYCYYGLKPPEGDPLGTFNF 389
Query: 1368 LIAQARFHILTNSVYQRLI------SSPSLTPEDTLKLQQPIEEWYNGLPSYLQ 1511
L A+ + + V + L S+ S E L+ + WYN LP +Q
Sbjct: 390 LEAEVQLTRIVGQVLKELYSVSGMKSNSSQVMEKILEFDLLLNNWYNSLPDCMQ 443
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 131
effective length of database: 253,773,515
effective search space used: 172312216685
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)