BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.CL.3.cl.339.Contig1
(2338 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_182027.1| hypothetical protein; protein id: At2g4500... 573 e-162
gb|AAM91808.1| unknown protein [Arabidopsis thaliana] 489 e-137
dbj|BAA24403.1| nuclear pore complex glycoprotein p62 [Onco... 99 4e-19
pir||A56573 nuclear pore complex glycoprotein p62 - mouse|g... 95 5e-18
emb|CAA41411.1| nucleoporin p62 [Homo sapiens] 94 7e-18
>ref|NP_182027.1| hypothetical protein; protein id: At2g45000.1, supported by cDNA:
gi_18491274 [Arabidopsis
thaliana]|gi|25408916|pir||D84885 hypothetical protein
At2g45000 [imported] - Arabidopsis
thaliana|gi|18491275|gb|AAL69462.1| At2g45000/T14P1.20
[Arabidopsis thaliana]|gi|19347759|gb|AAL86303.1| unknown
protein [Arabidopsis thaliana]
Length = 387
Score = 573 bits (1478), Expect = e-162
Identities = 300/385 (77%), Positives = 300/385 (77%)
Frame = -2
Query: 1197 NTAPDVLDAGAAELPVERLKPELADVPNEKXXXXXXXXXXXXXXXXXXXXXXXLFKKENP 1018
NTAPDVLDAGAAELPVERLKPELADVPNEK LFKKENP
Sbjct: 3 NTAPDVLDAGAAELPVERLKPELADVPNEKEDEDEAVCGGAEGVVELVVELAVLFKKENP 62
Query: 1017 VEAENGEAEAFEVLDVDEELKVDTPNGDGEEVVAPDEEDAKRDGVEPAEEDEPKNGEEPV 838
VEAENGEAEAFEVLDVDEELKVDTPNGDGEEVVAPDEEDAKRDGVEPAEEDEPKNGEEPV
Sbjct: 63 VEAENGEAEAFEVLDVDEELKVDTPNGDGEEVVAPDEEDAKRDGVEPAEEDEPKNGEEPV 122
Query: 837 APKIDGEEPEADEATLKEGDEPVAPKMDDEEPGADEATLKDGVAPVADDGAPKRGDVEVA 658
APKIDGEEPEADEATLKEGDEPVAPKMDDEEPGADEATLKDGVAPVADDGAPKRGDVEVA
Sbjct: 123 APKIDGEEPEADEATLKEGDEPVAPKMDDEEPGADEATLKDGVAPVADDGAPKRGDVEVA 182
Query: 657 ADEDAPKRXXXXXXXXXXXENKEGEDPKRGVEPLAGAAPNXXXXXXXXXXXXAPNRDDDG 478
ADEDAPKR ENKEGEDPKRGVEPLAGAAPN APNRDDDG
Sbjct: 183 ADEDAPKREELEAEADEGAENKEGEDPKRGVEPLAGAAPNGDDDGVAAEEAGAPNRDDDG 242
Query: 477 XXXXXXXXVTNPKXXXXXXXXXALVVDEPNSVGAGADEEAAVPNPNDGVVEAGVTEAEDP 298
VTNPK ALVVDEPNS GAGADEEAAVPNPNDGVVEAGVTEAEDP
Sbjct: 243 VAVEEAEEVTNPKGEEPGAAEEALVVDEPNSDGAGADEEAAVPNPNDGVVEAGVTEAEDP 302
Query: 297 NPNDGVDEEAEEPNPKEGVEEAPNPNDGVVEEAGVDDTEDPKPNPVELGFEDWLKEKXXX 118
NPNDGVDEEAEEPNPKEGVEEAPNPNDGVVEEAGVDDTEDPKPNPVELGFEDWLKEK
Sbjct: 303 NPNDGVDEEAEEPNPKEGVEEAPNPNDGVVEEAGVDDTEDPKPNPVELGFEDWLKEKDNG 362
Query: 117 XXXXXXXXXXXXXXXXXXNPPTELD 43
NPPTELD
Sbjct: 363 EVVDEAEEELVAEEDPNENPPTELD 387
>gb|AAM91808.1| unknown protein [Arabidopsis thaliana]
Length = 739
Score = 489 bits (1260), Expect = e-137
Identities = 272/521 (52%), Positives = 272/521 (52%)
Frame = +2
Query: 674 PLFGAPSSATGATXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXIFXXXXXXX 853
PLFGAPSSATGAT IF
Sbjct: 219 PLFGAPSSATGATPSFSVASSAPGSSSSIFGATGSSPSFSVASSASGSSPSIFGATGSSP 278
Query: 854 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVXXXXXXXXXXXXXXXXXXXXXXXGXXXX 1033
V G
Sbjct: 279 FFGSSSSAGSTPSLFASSSSGATTSSPSPFGVSTFNSSSTSNTSNASASPFSASTGFSFL 338
Query: 1034 XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXGFNLXXXXXXXXXXXXXXXVFSIXXX 1213
GFNL VFSI
Sbjct: 339 KSTASSTTSSTTPSAPPQTASSSSSFSFGTSANSGFNLSTGSSAAPASSTSGAVFSIATT 398
Query: 1214 XXXXXXXXXXXXXXXXXXXXXXXXXXXFGVXXXXXXXXXXXXXXXXXXXGFGLASSTPAT 1393
FGV GFGLASSTPAT
Sbjct: 399 TTTSSSTPAATSAPASSAPASTMAFPSFGVTSSATNTTPASSAATFSTTGFGLASSTPAT 458
Query: 1394 GSTNSFTGFAVPKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 1573
GSTNSFTGFAVPK
Sbjct: 459 GSTNSFTGFAVPKTSTPASSSQPQTTSPAFSFSLPSSTSTTAPATSSATTTQTTLVVPSS 518
Query: 1574 XGTSTAVAPVAGSPKLPSEITGKTVEEIIKEWNTELQERTGRFRKQANAIAEWDKRILQN 1753
GTSTAVAPVAGSPKLPSEITGKTVEEIIKEWNTELQERTGRFRKQANAIAEWDKRILQN
Sbjct: 519 SGTSTAVAPVAGSPKLPSEITGKTVEEIIKEWNTELQERTGRFRKQANAIAEWDKRILQN 578
Query: 1754 RDVLLRLEIEVAKVVETQSSLERQLELIETHQQEVDKALQSMEEEAERIYNDERKSLLDD 1933
RDVLLRLEIEVAKVVETQSSLERQLELIETHQQEVDKALQSMEEEAERIYNDERKSLLDD
Sbjct: 579 RDVLLRLEIEVAKVVETQSSLERQLELIETHQQEVDKALQSMEEEAERIYNDERKSLLDD 638
Query: 1934 EAASTRDAMYEQSELVERELEHMTEQIRSIIQSVNANQGGELEAIDGMSPLDVVVRILNN 2113
EAASTRDAMYEQSELVERELEHMTEQIRSIIQSVNANQGGELEAIDGMSPLDVVVRILNN
Sbjct: 639 EAASTRDAMYEQSELVERELEHMTEQIRSIIQSVNANQGGELEAIDGMSPLDVVVRILNN 698
Query: 2114 QLSSLMWIDEKAEEFSSRIQKIALQGSGGDRELMAPKHWMS 2236
QLSSLMWIDEKAEEFSSRIQKIALQGSGGDRELMAPKHWMS
Sbjct: 699 QLSSLMWIDEKAEEFSSRIQKIALQGSGGDRELMAPKHWMS 739
>dbj|BAA24403.1| nuclear pore complex glycoprotein p62 [Oncorhynchus mykiss]
Length = 579
Score = 98.6 bits (244), Expect = 4e-19
Identities = 53/202 (26%), Positives = 109/202 (53%)
Frame = +2
Query: 1577 GTSTAVAPVAGSPKLPSEITGKTVEEIIKEWNTELQERTGRFRKQANAIAEWDKRILQNR 1756
G +T +A V S P ++ +E +I +W+ EL+++ F +QA + WD+ +++N
Sbjct: 369 GVATTIATVTASA--PPVMSYMQLEGLINKWSLELEDQERHFLQQATQVNAWDRTLVENG 426
Query: 1757 DVLLRLEIEVAKVVETQSSLERQLELIETHQQEVDKALQSMEEEAERIYNDERKSLLDDE 1936
+ + L E+ KV Q L+++L+ I + Q+E++ L +EE + ++ ++
Sbjct: 427 EKITSLHREMEKVKLDQRRLDQELDFILSQQKELEDLLSPLEESVK----EQSGTIYMQN 482
Query: 1937 AASTRDAMYEQSELVERELEHMTEQIRSIIQSVNANQGGELEAIDGMSPLDVVVRILNNQ 2116
A R+ Y+ +E V+ +L+ M++ ++ II+ +N + G D PL + +ILN
Sbjct: 483 ADEERERTYKLAENVDAQLKRMSQDLKEIIEHLNTSSG----PADNSDPLQQICKILNAH 538
Query: 2117 LSSLMWIDEKAEEFSSRIQKIA 2182
+ SL WID+ + R++ ++
Sbjct: 539 MDSLQWIDQNSVLLQRRVEDVS 560
>pir||A56573 nuclear pore complex glycoprotein p62 -
mouse|gi|236261|gb|AAB19953.1| nuclear pore complex
glycoprotein p62 [Mus sp.]
Length = 526
Score = 94.7 bits (234), Expect = 5e-18
Identities = 53/210 (25%), Positives = 109/210 (51%)
Frame = +2
Query: 1580 TSTAVAPVAGSPKLPSEITGKTVEEIIKEWNTELQERTGRFRKQANAIAEWDKRILQNRD 1759
T+ V+ A +T +E +I +W+ EL+++ F +QA + WD+ +++N +
Sbjct: 315 TALPVSSTAAGTATGPAMTYAQLESLINKWSLELEDQERHFLQQATQVNAWDRTLIENGE 374
Query: 1760 VLLRLEIEVAKVVETQSSLERQLELIETHQQEVDKALQSMEEEAERIYNDERKSLLDDEA 1939
+ L EV KV Q L+++L+ I + Q+E++ L +EE + ++ ++ A
Sbjct: 375 KITSLHREVEKVKLDQKRLDQELDFILSQQKELEDLLSPLEESVK----EQSGTIYLQHA 430
Query: 1940 ASTRDAMYEQSELVERELEHMTEQIRSIIQSVNANQGGELEAIDGMSPLDVVVRILNNQL 2119
R+ Y+ +E ++ +L+ M + ++ II+ +N GG + D PL + +ILN +
Sbjct: 431 DEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNM-AGGPADTSD---PLQQICKILNAHM 486
Query: 2120 SSLMWIDEKAEEFSSRIQKIALQGSGGDRE 2209
SL W+D+ + R+++ + G +E
Sbjct: 487 DSLQWVDQSSALLQRRVEEASRVCEGRRKE 516
>emb|CAA41411.1| nucleoporin p62 [Homo sapiens]
Length = 522
Score = 94.4 bits (233), Expect = 7e-18
Identities = 48/185 (25%), Positives = 100/185 (53%)
Frame = +2
Query: 1625 SEITGKTVEEIIKEWNTELQERTGRFRKQANAIAEWDKRILQNRDVLLRLEIEVAKVVET 1804
S +T +E +I +W+ EL+++ F +QA + WD+ +++N + + L EV KV
Sbjct: 326 SAMTYAQLESLINKWSLELEDQERHFLQQATQVNAWDRTLIENGEKITSLHREVEKVKLD 385
Query: 1805 QSSLERQLELIETHQQEVDKALQSMEEEAERIYNDERKSLLDDEAASTRDAMYEQSELVE 1984
Q L+++L+ I + Q+E++ L +EE + ++R ++ A R Y+ +E ++
Sbjct: 386 QKRLDQELDFILSQQKELEDLLSPLEE----LVKEQRATIYLQHADEERQKTYKLAENID 441
Query: 1985 RELEHMTEQIRSIIQSVNANQGGELEAIDGMSPLDVVVRILNNQLSSLMWIDEKAEEFSS 2164
+L+ M + ++ II+ +N + D PL + +ILN + SL WID+ +
Sbjct: 442 AQLKRMAQDLKDIIEHLNTSGA----PADTSDPLQQICKILNAHMDSLQWIDQNSALLQR 497
Query: 2165 RIQKI 2179
+++++
Sbjct: 498 KVEEV 502
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 131
effective length of database: 253,773,515
effective search space used: 164191464205
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)