BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.CL.1.cl.173.Contig1
(1297 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_175988.1| serine acetyltransferase; protein id: At1g... 635 0.0
pir||S71181 serine O-acetyltransferase (EC 2.3.1.30) SAT1 p... 623 e-177
emb|CAA56913.1| serine O-acetyltransferase [Arabidopsis tha... 465 e-130
ref|NP_187918.1| serine acetyltransferase (Sat-1); protein ... 465 e-130
gb|AAB02050.1| serine acetyltransferase 462 e-129
>ref|NP_175988.1| serine acetyltransferase; protein id: At1g55920.1, supported by cDNA:
gi_926938 [Arabidopsis thaliana]|gi|2146776|pir||S67482
serine O-acetyltransferase (EC 2.3.1.30), cytosolic -
Arabidopsis thaliana|gi|608577|gb|AAA58608.1| serine
acetyltransferase|gi|608677|emb|CAA84371.1| serine
acetyltransferase [Arabidopsis
thaliana]|gi|8778310|gb|AAF79319.1|AC002304_12 F14J16.18
[Arabidopsis thaliana]|gi|1093493|prf||2104212A Ser
acetyltransferase
Length = 314
Score = 635 bits (1637), Expect = 0.0
Identities = 313/314 (99%), Positives = 314/314 (99%)
Frame = -2
Query: 1035 MATCIDTCRTGNTQEDDSRFCCIKNFFRPGFSVNRKIHHTQIEDDDDVWIKMLEEAKSDV 856
MATCIDTCRTGNTQ+DDSRFCCIKNFFRPGFSVNRKIHHTQIEDDDDVWIKMLEEAKSDV
Sbjct: 1 MATCIDTCRTGNTQDDDSRFCCIKNFFRPGFSVNRKIHHTQIEDDDDVWIKMLEEAKSDV 60
Query: 855 KQEPILSNYYYASITSHRSLESALAHILSVKLSNLNLPSNTLFELFISVLEESPEIIEST 676
KQEPILSNYYYASITSHRSLESALAHILSVKLSNLNLPSNTLFELFISVLEESPEIIEST
Sbjct: 61 KQEPILSNYYYASITSHRSLESALAHILSVKLSNLNLPSNTLFELFISVLEESPEIIEST 120
Query: 675 KQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHTLWKQNRKIVALLIQNRVSESFA 496
KQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHTLWKQNRKIVALLIQNRVSESFA
Sbjct: 121 KQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHTLWKQNRKIVALLIQNRVSESFA 180
Query: 495 VDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHGVTLGGTGKQSGDRHPKIGDGVL 316
VDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHGVTLGGTGKQSGDRHPKIGDGVL
Sbjct: 181 VDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHGVTLGGTGKQSGDRHPKIGDGVL 240
Query: 315 IGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGNPARLIGGKENPRKHDKIPCLTM 136
IGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGNPARLIGGKENPRKHDKIPCLTM
Sbjct: 241 IGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGNPARLIGGKENPRKHDKIPCLTM 300
Query: 135 DQTSYLTEWSDYVI 94
DQTSYLTEWSDYVI
Sbjct: 301 DQTSYLTEWSDYVI 314
>pir||S71181 serine O-acetyltransferase (EC 2.3.1.30) SAT1 precursor, chloroplast
- Arabidopsis thaliana|gi|926939|gb|AAC37474.1| serine
acetyltransferase
Length = 314
Score = 623 bits (1607), Expect = e-177
Identities = 307/314 (97%), Positives = 310/314 (97%)
Frame = -2
Query: 1035 MATCIDTCRTGNTQEDDSRFCCIKNFFRPGFSVNRKIHHTQIEDDDDVWIKMLEEAKSDV 856
MATCI TCRTGNTQ+DDSRFCCI FFRPGFSVNRKIHHTQIEDDDDVWIKML+EA+SDV
Sbjct: 1 MATCIHTCRTGNTQDDDSRFCCINKFFRPGFSVNRKIHHTQIEDDDDVWIKMLKEAESDV 60
Query: 855 KQEPILSNYYYASITSHRSLESALAHILSVKLSNLNLPSNTLFELFISVLEESPEIIEST 676
KQEPILSNYYYASITSHRSLESAL HILSVKLSNLNLPSNTLFELFISVLEESPEIIEST
Sbjct: 61 KQEPILSNYYYASITSHRSLESALGHILSVKLSNLNLPSNTLFELFISVLEESPEIIEST 120
Query: 675 KQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHTLWKQNRKIVALLIQNRVSESFA 496
KQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHTLWKQNRKIVALLIQNRVSESFA
Sbjct: 121 KQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHTLWKQNRKIVALLIQNRVSESFA 180
Query: 495 VDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHGVTLGGTGKQSGDRHPKIGDGVL 316
VDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHGVTLGGTGKQSGDRHPKIGDGVL
Sbjct: 181 VDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHGVTLGGTGKQSGDRHPKIGDGVL 240
Query: 315 IGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGNPARLIGGKENPRKHDKIPCLTM 136
IGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGNPARLIGGKENPRKHDKIPCLTM
Sbjct: 241 IGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGNPARLIGGKENPRKHDKIPCLTM 300
Query: 135 DQTSYLTEWSDYVI 94
DQTSYLTEWSDYVI
Sbjct: 301 DQTSYLTEWSDYVI 314
>emb|CAA56913.1| serine O-acetyltransferase [Arabidopsis thaliana]
Length = 336
Score = 465 bits (1196), Expect = e-130
Identities = 241/336 (71%), Positives = 273/336 (80%), Gaps = 22/336 (6%)
Frame = -2
Query: 1035 MATCIDTCRTGNTQ----------EDDSRFCCIKNFFRPGFS----VNRKIHHTQ--IED 904
MA CIDTCRTG Q +D+S F + F P S K HT+ +ED
Sbjct: 1 MAACIDTCRTGKPQISPRDSSKHHDDESGFRYMNYFRYPDRSSFNGTQTKTLHTRPLLED 60
Query: 903 ------DDDVWIKMLEEAKSDVKQEPILSNYYYASITSHRSLESALAHILSVKLSNLNLP 742
DDVW K+ EEAKSD+ +EPI+S YY+ASI S RSLE+ALA+ LSVKLSNLNLP
Sbjct: 61 LDRDAEVDDVWAKIREEAKSDIAKEPIVSAYYHASIVSQRSLEAALANTLSVKLSNLNLP 120
Query: 741 SNTLFELFISVLEESPEIIESTKQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHT 562
SNTLF+LF VL+ +P+I+ES K DL+AVKERDPACISYVHCFL FKGFLACQAHRIAH
Sbjct: 121 SNTLFDLFSGVLQGNPDIVESVKLDLLAVKERDPACISYVHCFLHFKGFLACQAHRIAHE 180
Query: 561 LWKQNRKIVALLIQNRVSESFAVDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHG 382
LW Q+RKI+ALLIQNRVSE+FAVD HPGAKIG GILLDHAT +VIGETAVVG+NVSILH
Sbjct: 181 LWTQDRKILALLIQNRVSEAFAVDFHPGAKIGTGILLDHATAIVIGETAVVGNNVSILHN 240
Query: 381 VTLGGTGKQSGDRHPKIGDGVLIGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGN 202
VTLGGTGKQ GDRHPKIGDGVLIGAG+CILGNITIGEGAKIG+GSVV+KDVP RTTAVGN
Sbjct: 241 VTLGGTGKQCGDRHPKIGDGVLIGAGTCILGNITIGEGAKIGAGSVVLKDVPPRTTAVGN 300
Query: 201 PARLIGGKENPRKHDKIPCLTMDQTSYLTEWSDYVI 94
PARL+GGK+NP+ HDKIP LTMDQTS+++EWSDYVI
Sbjct: 301 PARLLGGKDNPKTHDKIPGLTMDQTSHISEWSDYVI 336
>ref|NP_187918.1| serine acetyltransferase (Sat-1); protein id: At3g13110.1, supported
by cDNA: gi_1184047 [Arabidopsis
thaliana]|gi|2129733|pir||S69192 serine
O-acetyltransferase (EC 2.3.1.30) SAT-1a precursor,
chloroplast - Arabidopsis
thaliana|gi|1184048|gb|AAB07778.1| serine
acetyltransferase [Arabidopsis
thaliana]|gi|10172598|dbj|BAB01402.1| serine
O-acetyltransferase (EC 2.3.1.30) SAT1 precursor
[Arabidopsis thaliana]
Length = 391
Score = 465 bits (1196), Expect = e-130
Identities = 241/336 (71%), Positives = 273/336 (80%), Gaps = 22/336 (6%)
Frame = -2
Query: 1035 MATCIDTCRTGNTQ----------EDDSRFCCIKNFFRPGFS----VNRKIHHTQ--IED 904
MA CIDTCRTG Q +D+S F + F P S K HT+ +ED
Sbjct: 56 MAACIDTCRTGKPQISPRDSSKHHDDESGFRYMNYFRYPDRSSFNGTQTKTLHTRPLLED 115
Query: 903 ------DDDVWIKMLEEAKSDVKQEPILSNYYYASITSHRSLESALAHILSVKLSNLNLP 742
DDVW K+ EEAKSD+ +EPI+S YY+ASI S RSLE+ALA+ LSVKLSNLNLP
Sbjct: 116 LDRDAEVDDVWAKIREEAKSDIAKEPIVSAYYHASIVSQRSLEAALANTLSVKLSNLNLP 175
Query: 741 SNTLFELFISVLEESPEIIESTKQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHT 562
SNTLF+LF VL+ +P+I+ES K DL+AVKERDPACISYVHCFL FKGFLACQAHRIAH
Sbjct: 176 SNTLFDLFSGVLQGNPDIVESVKLDLLAVKERDPACISYVHCFLHFKGFLACQAHRIAHE 235
Query: 561 LWKQNRKIVALLIQNRVSESFAVDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHG 382
LW Q+RKI+ALLIQNRVSE+FAVD HPGAKIG GILLDHAT +VIGETAVVG+NVSILH
Sbjct: 236 LWTQDRKILALLIQNRVSEAFAVDFHPGAKIGTGILLDHATAIVIGETAVVGNNVSILHN 295
Query: 381 VTLGGTGKQSGDRHPKIGDGVLIGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGN 202
VTLGGTGKQ GDRHPKIGDGVLIGAG+CILGNITIGEGAKIG+GSVV+KDVP RTTAVGN
Sbjct: 296 VTLGGTGKQCGDRHPKIGDGVLIGAGTCILGNITIGEGAKIGAGSVVLKDVPPRTTAVGN 355
Query: 201 PARLIGGKENPRKHDKIPCLTMDQTSYLTEWSDYVI 94
PARL+GGK+NP+ HDKIP LTMDQTS+++EWSDYVI
Sbjct: 356 PARLLGGKDNPKTHDKIPGLTMDQTSHISEWSDYVI 391
>gb|AAB02050.1| serine acetyltransferase
Length = 336
Score = 462 bits (1189), Expect = e-129
Identities = 240/336 (71%), Positives = 272/336 (80%), Gaps = 22/336 (6%)
Frame = -2
Query: 1035 MATCIDTCRTGNTQ----------EDDSRFCCIKNFFRPGFS----VNRKIHHTQ--IED 904
MA CIDTCRTG Q +D+S F + F P S K HT+ +ED
Sbjct: 1 MAACIDTCRTGKPQISPRDSSKHHDDESGFRYMNYFRYPDRSSFNGTQTKTLHTRPLLED 60
Query: 903 ------DDDVWIKMLEEAKSDVKQEPILSNYYYASITSHRSLESALAHILSVKLSNLNLP 742
DDVW K+ EEAKSD+ +EPI+S YY+ASI S RSLE+ALA+ LSVKLSNLNLP
Sbjct: 61 LDRDAEVDDVWAKIREEAKSDIAKEPIVSAYYHASIVSQRSLEAALANTLSVKLSNLNLP 120
Query: 741 SNTLFELFISVLEESPEIIESTKQDLIAVKERDPACISYVHCFLGFKGFLACQAHRIAHT 562
SNTLF+LF L+ +P+I+ES K DL+AVKERDPACISYVHCFL FKGFLACQAHRIAH
Sbjct: 121 SNTLFDLFSGGLQGNPDIVESVKLDLLAVKERDPACISYVHCFLHFKGFLACQAHRIAHE 180
Query: 561 LWKQNRKIVALLIQNRVSESFAVDIHPGAKIGKGILLDHATGVVIGETAVVGDNVSILHG 382
LW Q+RKI+ALLIQNRVSE+FAVD HPGAKIG GILLDHAT +VIGETAVVG+NVSILH
Sbjct: 181 LWTQDRKILALLIQNRVSEAFAVDFHPGAKIGTGILLDHATAIVIGETAVVGNNVSILHN 240
Query: 381 VTLGGTGKQSGDRHPKIGDGVLIGAGSCILGNITIGEGAKIGSGSVVVKDVPARTTAVGN 202
VTLGGTGKQ GDRHPKIGDGVLIGAG+CILGNITIGEGAKIG+GSVV+KDVP RTTAVGN
Sbjct: 241 VTLGGTGKQCGDRHPKIGDGVLIGAGTCILGNITIGEGAKIGAGSVVLKDVPPRTTAVGN 300
Query: 201 PARLIGGKENPRKHDKIPCLTMDQTSYLTEWSDYVI 94
PARL+GGK+NP+ HDKIP LTMDQTS+++EWSDYVI
Sbjct: 301 PARLLGGKDNPKTHDKIPGLTMDQTSHISEWSDYVI 336
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 126
effective length of database: 260,468,745
effective search space used: 79442967225
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)