BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.CL.1.cl.116.Contig1
(2046 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
pir||G96588 hypothetical protein T22H22.10 [imported] - Ara... 451 0.0
ref|NP_197784.1| terpene synthase/cyclase family; protein i... 269 e-166
dbj|BAB08719.1| (+)-delta-cadinene synthase (d-cadinene syn... 252 e-161
gb|AAL15301.1| unknown protein [Arabidopsis thaliana] 462 e-129
dbj|BAA82092.1| vetispiradiene synthase [Solanum tuberosum] 196 2e-99
>pir||G96588 hypothetical protein T22H22.10 [imported] - Arabidopsis
thaliana|gi|3776563|gb|AAC64880.1| Strong similarity to
gb|L04680 5-epi-aristolochene synthase from Nicotiana
tabacum and delta cadinene syntase gb|U88318 from
Gosssypium hirsutum. [Arabidopsis thaliana]
Length = 530
Score = 451 bits (1161), Expect(3) = 0.0
Identities = 223/231 (96%), Positives = 226/231 (97%)
Frame = +3
Query: 1146 ISLIMWNPSGIEGLPESMRYLHHVVLDFYGKLEEDMEREGRSGCGLFAKKSMIVTAKAYL 1325
I L + + +GIEGLPESMRYLHHVVLDFYGKLEEDMEREGRSGCGLFAKKSMIVTAKAYL
Sbjct: 300 ILLTLVDDTGIEGLPESMRYLHHVVLDFYGKLEEDMEREGRSGCGLFAKKSMIVTAKAYL 359
Query: 1326 QEAKWLSEDFVATFDEYKENGVYSSSYLALLTGSFLGMVDEGTLDVFEWLSTFPPLLVTS 1505
QEAKWLSEDFVATFDEYKENGVYSSSYLALLTGSFLGMVDEGTLDVFEWLSTFPPLLVTS
Sbjct: 360 QEAKWLSEDFVATFDEYKENGVYSSSYLALLTGSFLGMVDEGTLDVFEWLSTFPPLLVTS 419
Query: 1506 ALIARLCGDIASCEFEHKRKHVGTSIDCYMKQYGISWEKAEEEIKIMALDSWKSLNQELM 1685
ALI RLCGDIASCEFEHKRKHVGTSIDCYMKQYGISWEKAEEEIKIMALDSWKSLNQELM
Sbjct: 420 ALIGRLCGDIASCEFEHKRKHVGTSIDCYMKQYGISWEKAEEEIKIMALDSWKSLNQELM 479
Query: 1686 TRDHSFAFPIVMRFLNLSRVVKVLYKDTDIFTHPELMKHHVVSLFLNRISI 1838
TRDHSFAFPIVMRFLNLSRVVKVLYKDTDIFTHPELMKHHVVSLFLNRISI
Sbjct: 480 TRDHSFAFPIVMRFLNLSRVVKVLYKDTDIFTHPELMKHHVVSLFLNRISI 530
Score = 373 bits (957), Expect(3) = 0.0
Identities = 195/242 (80%), Positives = 196/242 (80%)
Frame = +1
Query: 385 MQMIRGNEFDLYTVSLVFQIFRQFGYKLGVDVXXXXXXXXXXXXEHLAEDARGLLCLYEA 564
+ MIRGNEFDLYTVSLVFQIFRQFGYKLGVDV EHLAEDARGLLCLYEA
Sbjct: 100 VMMIRGNEFDLYTVSLVFQIFRQFGYKLGVDVFNNFKNKDGKFKEHLAEDARGLLCLYEA 159
Query: 565 AHWSTHGEDILDEALAFSRSHLEGLADQSSPPMSIRIKNALKHAYPRGISRIETRQYISY 744
AHWSTHGEDILDEALAFSRSHLEGLADQSSPPMSIRIKNALKHAYPRGISRIETRQYISY
Sbjct: 160 AHWSTHGEDILDEALAFSRSHLEGLADQSSPPMSIRIKNALKHAYPRGISRIETRQYISY 219
Query: 745 YEEEDLHDQTLLEFAKIDFNLLQILHRKELCQVFRCHYELAR*IIYTF*NSYIWRDYHND 924
YEEEDLHDQTLLEFAKIDFNLLQILHRKELCQVF
Sbjct: 220 YEEEDLHDQTLLEFAKIDFNLLQILHRKELCQVF-------------------------- 253
Query: 925 CRFFFFCRWYSNLELDLKVPYARNRTVESYLWAVGAYFEPRYSQARIRLAIVVILLTLVD 1104
RWYSNLELDLKVPYARNRTVESYLWAVGAYFEPRYSQARIRLAIVVILLTLVD
Sbjct: 254 -------RWYSNLELDLKVPYARNRTVESYLWAVGAYFEPRYSQARIRLAIVVILLTLVD 306
Query: 1105 DT 1110
DT
Sbjct: 307 DT 308
Score = 202 bits (513), Expect(3) = 0.0
Identities = 101/103 (98%), Positives = 103/103 (99%)
Frame = +2
Query: 77 MQELINTLSALTTEEIYRPLADFPVNIWEDPLISFSVLDPESEINEEKLSSLKETVRGLL 256
MQELINTLSALTTEEIYRPLADFPVNIWEDPLISFSVLDPESEINEEKLSSLKETVRGLL
Sbjct: 1 MQELINTLSALTTEEIYRPLADFPVNIWEDPLISFSVLDPESEINEEKLSSLKETVRGLL 60
Query: 257 MASKEEPIENIKFIDALCRLGVSYHFEVDIFTQLETMFGVMVL 385
MASKEEPIENIKFIDALCRLGVSYHFEVDIFTQLETMFGVM++
Sbjct: 61 MASKEEPIENIKFIDALCRLGVSYHFEVDIFTQLETMFGVMMI 103
>ref|NP_197784.1| terpene synthase/cyclase family; protein id: At5g23960.1 [Arabidopsis
thaliana]
Length = 545
Score = 269 bits (687), Expect(3) = e-166
Identities = 140/259 (54%), Positives = 168/259 (64%)
Frame = +1
Query: 373 CHGFMQMIRGNEFDLYTVSLVFQIFRQFGYKLGVDVXXXXXXXXXXXXEHLAEDARGLLC 552
C F QM+R DLYTV ++FQ+FRQFG+KL DV HL DA G+L
Sbjct: 89 CLDFPQMVRQEGCDLYTVGIIFQVFRQFGFKLSADVFEKFKDENGKFKGHLVTDAYGMLS 148
Query: 553 LYEAAHWSTHGEDILDEALAFSRSHLEGLADQSSPPMSIRIKNALKHAYPRGISRIETRQ 732
LYEAA W THGEDI+DEALAFSRSHLE ++ +SSP ++IRIKNALKH Y +GISRIETRQ
Sbjct: 149 LYEAAQWGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETRQ 208
Query: 733 YISYYEEEDLHDQTLLEFAKIDFNLLQILHRKELCQVFRCHYELAR*IIYTF*NSYIWRD 912
YISYYEEE+ D TLLEFAKIDFNLLQILHR+EL V
Sbjct: 209 YISYYEEEESCDPTLLEFAKIDFNLLQILHREELACV----------------------- 245
Query: 913 YHNDCRFFFFCRWYSNLELDLKVPYARNRTVESYLWAVGAYFEPRYSQARIRLAIVVILL 1092
RW+ +E KV Y R+R E+YLW++G YFEP+YSQAR+ + +IL
Sbjct: 246 ----------TRWHHEMEFKSKVTYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILF 295
Query: 1093 TLVDDTYDAFGTIEELEPF 1149
T +DD YDA+GT+EELE F
Sbjct: 296 TALDDMYDAYGTMEELELF 314
Score = 252 bits (644), Expect(3) = e-166
Identities = 117/218 (53%), Positives = 162/218 (73%)
Frame = +3
Query: 1185 LPESMRYLHHVVLDFYGKLEEDMEREGRSGCGLFAKKSMIVTAKAYLQEAKWLSEDFVAT 1364
+P+SM+++++V ++FY KL+E++E+EGRSGCG KKS+ TA Y+QEAKWL +D++AT
Sbjct: 329 IPDSMKFIYNVTVEFYDKLDEELEKEGRSGCGFHLKKSLQKTANGYMQEAKWLKKDYIAT 388
Query: 1365 FDEYKENGVYSSSYLALLTGSFLGMVDEGTLDVFEWLSTFPPLLVTSALIARLCGDIASC 1544
FDEYKEN + SS Y AL+ +F+ M D LD FEWLS+ P + V S +I+R DI+S
Sbjct: 389 FDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEIISRFTDDISSY 448
Query: 1545 EFEHKRKHVGTSIDCYMKQYGISWEKAEEEIKIMALDSWKSLNQELMTRDHSFAFPIVMR 1724
EFEHKR+HV T IDCYM+Q+G+S E+A E + + D+WK LNQELM R H F FP++MR
Sbjct: 449 EFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDAWKDLNQELM-RPHVFPFPLLMR 507
Query: 1725 FLNLSRVVKVLYKDTDIFTHPELMKHHVVSLFLNRISI 1838
LNLSRV+ V Y+ D +T+P+L+K H+VSL + I I
Sbjct: 508 VLNLSRVIDVFYRYQDAYTNPKLLKEHIVSLLIETIPI 545
Score = 111 bits (278), Expect(3) = e-166
Identities = 56/84 (66%), Positives = 63/84 (74%)
Frame = +2
Query: 119 EIYRPLADFPVNIWEDPLISFSVLDPESEINEEKLSSLKETVRGLLMASKEEPIENIKFI 298
E+ RPLADFP NIWEDPL SFS D +E +EK S+LKE V+ M+SK PIENIKFI
Sbjct: 4 EVNRPLADFPANIWEDPLTSFSKSDLGTETFKEKHSTLKEAVKEAFMSSKANPIENIKFI 63
Query: 299 DALCRLGVSYHFEVDIFTQLETMF 370
DALCRLGVSYHFE DI QL+ F
Sbjct: 64 DALCRLGVSYHFEKDIVEQLDKSF 87
>dbj|BAB08719.1| (+)-delta-cadinene synthase (d-cadinene synthase) [Arabidopsis
thaliana]
Length = 522
Score = 252 bits (644), Expect(3) = e-161
Identities = 117/218 (53%), Positives = 162/218 (73%)
Frame = +3
Query: 1185 LPESMRYLHHVVLDFYGKLEEDMEREGRSGCGLFAKKSMIVTAKAYLQEAKWLSEDFVAT 1364
+P+SM+++++V ++FY KL+E++E+EGRSGCG KKS+ TA Y+QEAKWL +D++AT
Sbjct: 306 IPDSMKFIYNVTVEFYDKLDEELEKEGRSGCGFHLKKSLQKTANGYMQEAKWLKKDYIAT 365
Query: 1365 FDEYKENGVYSSSYLALLTGSFLGMVDEGTLDVFEWLSTFPPLLVTSALIARLCGDIASC 1544
FDEYKEN + SS Y AL+ +F+ M D LD FEWLS+ P + V S +I+R DI+S
Sbjct: 366 FDEYKENAILSSGYYALIAMTFVRMTDVAKLDAFEWLSSHPKIRVASEIISRFTDDISSY 425
Query: 1545 EFEHKRKHVGTSIDCYMKQYGISWEKAEEEIKIMALDSWKSLNQELMTRDHSFAFPIVMR 1724
EFEHKR+HV T IDCYM+Q+G+S E+A E + + D+WK LNQELM R H F FP++MR
Sbjct: 426 EFEHKREHVATGIDCYMQQFGVSKERAVEVMGNIVSDAWKDLNQELM-RPHVFPFPLLMR 484
Query: 1725 FLNLSRVVKVLYKDTDIFTHPELMKHHVVSLFLNRISI 1838
LNLSRV+ V Y+ D +T+P+L+K H+VSL + I I
Sbjct: 485 VLNLSRVIDVFYRYQDAYTNPKLLKEHIVSLLIETIPI 522
Score = 250 bits (639), Expect(3) = e-161
Identities = 131/246 (53%), Positives = 157/246 (63%)
Frame = +1
Query: 373 CHGFMQMIRGNEFDLYTVSLVFQIFRQFGYKLGVDVXXXXXXXXXXXXEHLAEDARGLLC 552
C F QM+R DLYTV ++FQ+FRQFG+KL DV HL DA G+L
Sbjct: 89 CLDFPQMVRQEGCDLYTVGIIFQVFRQFGFKLSADVFEKFKDENGKFKGHLVTDAYGMLS 148
Query: 553 LYEAAHWSTHGEDILDEALAFSRSHLEGLADQSSPPMSIRIKNALKHAYPRGISRIETRQ 732
LYEAA W THGEDI+DEALAFSRSHLE ++ +SSP ++IRIKNALKH Y +GISRIETRQ
Sbjct: 149 LYEAAQWGTHGEDIIDEALAFSRSHLEEISSRSSPHLAIRIKNALKHPYHKGISRIETRQ 208
Query: 733 YISYYEEEDLHDQTLLEFAKIDFNLLQILHRKELCQVFRCHYELAR*IIYTF*NSYIWRD 912
YISYYEEE+ D TLLEFAKIDFNLLQILHR+EL V
Sbjct: 209 YISYYEEEESCDPTLLEFAKIDFNLLQILHREELACV----------------------- 245
Query: 913 YHNDCRFFFFCRWYSNLELDLKVPYARNRTVESYLWAVGAYFEPRYSQARIRLAIVVILL 1092
RW+ +E KV Y R+R E+YLW++G YFEP+YSQAR+ + +IL
Sbjct: 246 ----------TRWHHEMEFKSKVTYTRHRITEAYLWSLGTYFEPQYSQARVITTMALILF 295
Query: 1093 TLVDDT 1110
T +DDT
Sbjct: 296 TALDDT 301
Score = 111 bits (278), Expect(3) = e-161
Identities = 56/84 (66%), Positives = 63/84 (74%)
Frame = +2
Query: 119 EIYRPLADFPVNIWEDPLISFSVLDPESEINEEKLSSLKETVRGLLMASKEEPIENIKFI 298
E+ RPLADFP NIWEDPL SFS D +E +EK S+LKE V+ M+SK PIENIKFI
Sbjct: 4 EVNRPLADFPANIWEDPLTSFSKSDLGTETFKEKHSTLKEAVKEAFMSSKANPIENIKFI 63
Query: 299 DALCRLGVSYHFEVDIFTQLETMF 370
DALCRLGVSYHFE DI QL+ F
Sbjct: 64 DALCRLGVSYHFEKDIVEQLDKSF 87
>gb|AAL15301.1| unknown protein [Arabidopsis thaliana]
Length = 227
Score = 462 bits (1189), Expect = e-129
Identities = 226/227 (99%), Positives = 226/227 (99%)
Frame = +3
Query: 1158 MWNPSGIEGLPESMRYLHHVVLDFYGKLEEDMEREGRSGCGLFAKKSMIVTAKAYLQEAK 1337
MWNPSGIEGLPESMRYLHHVVLDFYGKLEEDMEREGRSGCGLFAKKSMIVTAKAYLQEAK
Sbjct: 1 MWNPSGIEGLPESMRYLHHVVLDFYGKLEEDMEREGRSGCGLFAKKSMIVTAKAYLQEAK 60
Query: 1338 WLSEDFVATFDEYKENGVYSSSYLALLTGSFLGMVDEGTLDVFEWLSTFPPLLVTSALIA 1517
WLSEDFVATFDEYKENGVYSSSYLALLTGSFLGMVDEGTLDVFEWLSTFPPLLVTSALI
Sbjct: 61 WLSEDFVATFDEYKENGVYSSSYLALLTGSFLGMVDEGTLDVFEWLSTFPPLLVTSALIG 120
Query: 1518 RLCGDIASCEFEHKRKHVGTSIDCYMKQYGISWEKAEEEIKIMALDSWKSLNQELMTRDH 1697
RLCGDIASCEFEHKRKHVGTSIDCYMKQYGISWEKAEEEIKIMALDSWKSLNQELMTRDH
Sbjct: 121 RLCGDIASCEFEHKRKHVGTSIDCYMKQYGISWEKAEEEIKIMALDSWKSLNQELMTRDH 180
Query: 1698 SFAFPIVMRFLNLSRVVKVLYKDTDIFTHPELMKHHVVSLFLNRISI 1838
SFAFPIVMRFLNLSRVVKVLYKDTDIFTHPELMKHHVVSLFLNRISI
Sbjct: 181 SFAFPIVMRFLNLSRVVKVLYKDTDIFTHPELMKHHVVSLFLNRISI 227
>dbj|BAA82092.1| vetispiradiene synthase [Solanum tuberosum]
Length = 556
Score = 196 bits (497), Expect(3) = 2e-99
Identities = 101/244 (41%), Positives = 145/244 (59%)
Frame = +1
Query: 412 DLYTVSLVFQIFRQFGYKLGVDVXXXXXXXXXXXXEHLAEDARGLLCLYEAAHWSTHGED 591
DL T+S+ F++ RQ GY + + E L D +GLL LYEA+H THGED
Sbjct: 112 DLQTLSVQFRLLRQHGYNISPKLFIRFQDAKGKFKESLCNDIKGLLNLYEASHVRTHGED 171
Query: 592 ILDEALAFSRSHLEGLADQSSPPMSIRIKNALKHAYPRGISRIETRQYISYYEEEDLHDQ 771
IL+EALAFS +HLE A P+S ++ +AL+ + + I R+ETR +IS YEEE+ +
Sbjct: 172 ILEEALAFSTAHLESAAPHLKSPLSKQVTHALEQSLHKSIPRVETRYFISIYEEEEQKND 231
Query: 772 TLLEFAKIDFNLLQILHRKELCQVFRCHYELAR*IIYTF*NSYIWRDYHNDCRFFFFCRW 951
LL+FAK+DFNLLQ+LH++EL +V RW
Sbjct: 232 VLLQFAKLDFNLLQMLHKQELSEV---------------------------------SRW 258
Query: 952 YSNLELDLKVPYARNRTVESYLWAVGAYFEPRYSQARIRLAIVVILLTLVDDTYDAFGTI 1131
+ +L+ +PYAR+R VE Y W +G Y EP+YSQAR+ LA + ++++VDDT+DA+G +
Sbjct: 259 WKDLDFVTTLPYARDRAVECYFWTMGVYAEPQYSQARVMLAKTIAMISIVDDTFDAYGIV 318
Query: 1132 EELE 1143
+ELE
Sbjct: 319 KELE 322
Score = 155 bits (392), Expect(3) = 2e-99
Identities = 85/234 (36%), Positives = 137/234 (58%), Gaps = 2/234 (0%)
Frame = +3
Query: 1143 TISLIMWNPSGIEGLPESMRYLHHVVLDFYGKLEEDMEREGRSGCGLFAKKSMIVTAKAY 1322
T ++ W+ S I+ LP+ M+ + +LD Y E ++ ++GRS +AK+ M + Y
Sbjct: 325 TDAIQRWDISQIDRLPDYMKISYKALLDLYNDYEMELSKDGRSDVVHYAKERMKEIVRNY 384
Query: 1323 LQEAKWLSEDFVATFDEYKENGVYSSSYLALLTGSFLGMVDEGTLDVFEWLSTFPPLLVT 1502
EAKW E ++ EY N + +S+Y L T S+LGM D FEWL+ P +L
Sbjct: 385 FVEAKWFIEGYMPPVSEYLSNALATSTYYLLTTTSYLGMKSANKQD-FEWLAKNPKILEA 443
Query: 1503 SALIARLCGDIASCEFEHKRKHVGTSIDCYMKQYGISWEKAEEEIKIMALDSWKSLNQEL 1682
+ + R+ DIA+ E E R + T I+CYM+ YG+S EKA E+ + MA +WK +N+ +
Sbjct: 444 NVTLCRVIDDIATYEVEKGRGQIATGIECYMRDYGVSTEKAMEKFQEMAETAWKDVNEGI 503
Query: 1683 MTRDHSFAFPIVMRFLNLSRVVKVLYK-DTDIFTHPE-LMKHHVVSLFLNRISI 1838
+ R + I+ R LNL+R++ V YK + D +THPE ++K H+++L ++ I I
Sbjct: 504 L-RPTPVSTEILTRILNLARIIDVTYKHNQDGYTHPEKVLKPHIIALLVDSIEI 556
Score = 57.4 bits (137), Expect(3) = 2e-99
Identities = 32/86 (37%), Positives = 50/86 (57%), Gaps = 1/86 (1%)
Frame = +2
Query: 116 EEIYRPLADFPVNIWEDPLISFSVLDPESEINEEKLSSLKETVRGLLMASKEEPI-ENIK 292
EEI RP+ADF ++W D SFS+ + + +++ +LKE R +L AS + E +
Sbjct: 14 EEIVRPIADFSPSLWGDRFHSFSLDNQIAGKYAQEIETLKEQSRIILSASSRRTLAEKLD 73
Query: 293 FIDALCRLGVSYHFEVDIFTQLETMF 370
ID + RLG++YHFE I L+ +
Sbjct: 74 LIDIVERLGIAYHFEKQIDDMLDQFY 99
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 130
effective length of database: 255,112,561
effective search space used: 140567021111
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)