BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.CL.0.cl.064.Contig1
(1604 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AAK91459.1| At5g65691/F16M2.160 [Arabidopsis thaliana] 889 0.0
ref|NP_569018.1| Expressed protein; protein id: At5g65685.1... 886 0.0
pir||T05899 hypothetical protein F6H11.180 - Arabidopsis th... 473 e-132
gb|AAK98690.1|AC069158_2 Putative starch synthase [Oryza sa... 383 e-105
emb|CAB40375.1| starch synthase, isoform V [Vigna unguiculata] 249 9e-65
>gb|AAK91459.1| At5g65691/F16M2.160 [Arabidopsis thaliana]
Length = 460
Score = 889 bits (2297), Expect = 0.0
Identities = 445/460 (96%), Positives = 445/460 (96%)
Frame = +2
Query: 56 MQATANLPTIWNLSLRGSSSKVHIVNPRHRKSHALHCLRSEGHEEFDNSQKSLGQSSITK 235
MQATANLPTIWNLSLRGSSSKVHIVNPRHRKSHALHCLRSEGHEEFDNSQKSLGQSSITK
Sbjct: 1 MQATANLPTIWNLSLRGSSSKVHIVNPRHRKSHALHCLRSEGHEEFDNSQKSLGQSSITK 60
Query: 236 EAKHKDIWNLFREAQKNIMILNKQRLAAVXXXXXXXXXXXXXXXRINQLEAESQIVIKKD 415
EAKHKDIWNLFREAQKNIMILNKQRLAAV RINQLEAESQIVIKKD
Sbjct: 61 EAKHKDIWNLFREAQKNIMILNKQRLAAVDELEQLKKDKEELLERINQLEAESQIVIKKD 120
Query: 416 KSSLFWELLLRIDSMVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELR 595
KSSLFWELLLRIDSMVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELR
Sbjct: 121 KSSLFWELLLRIDSMVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELR 180
Query: 596 RFTNKGKRNGLHVIHICTEMAPLVSVEPLASYITGLSCALQEEGYMVEVILPKYSTLDLD 775
RFTNKGKRNGLHVIHICTEMAPLVSVEPLASYITGLSCALQEEGYMVEVILPKYSTLDLD
Sbjct: 181 RFTNKGKRNGLHVIHICTEMAPLVSVEPLASYITGLSCALQEEGYMVEVILPKYSTLDLD 240
Query: 776 EIEGLREIEADAYSYFDGQLHANRIWNGVVSGIGVTLIQPVYYSSMFSRDKVYGYQDDFD 955
EIEGLREIEADAYSYFDGQLHANRIWNGVVSGIGVTLIQPVYYSSMFSRDKVYGYQDDFD
Sbjct: 241 EIEGLREIEADAYSYFDGQLHANRIWNGVVSGIGVTLIQPVYYSSMFSRDKVYGYQDDFD 300
Query: 956 RFAYFSRASLDYIAKSGKQPDVLHIHNWQTAIVGPLFWDVFVNQGLEGTRILLTCQDFDK 1135
RFAYFSRASLDYIAKSGKQPDVLHIHNWQTAIVGPLFWDVFVNQGLEGTRILLTCQDFDK
Sbjct: 301 RFAYFSRASLDYIAKSGKQPDVLHIHNWQTAIVGPLFWDVFVNQGLEGTRILLTCQDFDK 360
Query: 1136 GLVPPEKLELCGLDPAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSIPGLE 1315
GLVPPEKLELCGLDPAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSIPGLE
Sbjct: 361 GLVPPEKLELCGLDPAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSIPGLE 420
Query: 1316 PTLAIHKDKLFFAPFGMDNSMEKDLCCDLHVSAYTSIKNL 1435
PTLAIHKDKLFFAPFGMDNSMEKDLCCDLHVSAYTSIKNL
Sbjct: 421 PTLAIHKDKLFFAPFGMDNSMEKDLCCDLHVSAYTSIKNL 460
>ref|NP_569018.1| Expressed protein; protein id: At5g65685.1, supported by cDNA:
gi_15215828 [Arabidopsis
thaliana]|gi|26452881|dbj|BAC43519.1| unknown protein
[Arabidopsis thaliana]
Length = 460
Score = 886 bits (2290), Expect = 0.0
Identities = 444/460 (96%), Positives = 444/460 (96%)
Frame = +2
Query: 56 MQATANLPTIWNLSLRGSSSKVHIVNPRHRKSHALHCLRSEGHEEFDNSQKSLGQSSITK 235
MQATANLPTIWNLSLRGSSSKVHIVNPRHRKSHALHCLRSEGHEEFDNSQKSLGQSSITK
Sbjct: 1 MQATANLPTIWNLSLRGSSSKVHIVNPRHRKSHALHCLRSEGHEEFDNSQKSLGQSSITK 60
Query: 236 EAKHKDIWNLFREAQKNIMILNKQRLAAVXXXXXXXXXXXXXXXRINQLEAESQIVIKKD 415
EAKHKDIWNLFREAQKNIMILNKQRLAAV RINQLEAESQIVIKKD
Sbjct: 61 EAKHKDIWNLFREAQKNIMILNKQRLAAVDELEQLKKDKEELLERINQLEAESQIVIKKD 120
Query: 416 KSSLFWELLLRIDSMVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELR 595
KSSLFWELLLRIDSMVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELR
Sbjct: 121 KSSLFWELLLRIDSMVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELR 180
Query: 596 RFTNKGKRNGLHVIHICTEMAPLVSVEPLASYITGLSCALQEEGYMVEVILPKYSTLDLD 775
RFTNKGKRNGLHVIHICTEMAPLVSV PLASYITGLSCALQEEGYMVEVILPKYSTLDLD
Sbjct: 181 RFTNKGKRNGLHVIHICTEMAPLVSVGPLASYITGLSCALQEEGYMVEVILPKYSTLDLD 240
Query: 776 EIEGLREIEADAYSYFDGQLHANRIWNGVVSGIGVTLIQPVYYSSMFSRDKVYGYQDDFD 955
EIEGLREIEADAYSYFDGQLHANRIWNGVVSGIGVTLIQPVYYSSMFSRDKVYGYQDDFD
Sbjct: 241 EIEGLREIEADAYSYFDGQLHANRIWNGVVSGIGVTLIQPVYYSSMFSRDKVYGYQDDFD 300
Query: 956 RFAYFSRASLDYIAKSGKQPDVLHIHNWQTAIVGPLFWDVFVNQGLEGTRILLTCQDFDK 1135
RFAYFSRASLDYIAKSGKQPDVLHIHNWQTAIVGPLFWDVFVNQGLEGTRILLTCQDFDK
Sbjct: 301 RFAYFSRASLDYIAKSGKQPDVLHIHNWQTAIVGPLFWDVFVNQGLEGTRILLTCQDFDK 360
Query: 1136 GLVPPEKLELCGLDPAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSIPGLE 1315
GLVPPEKLELCGLDPAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSIPGLE
Sbjct: 361 GLVPPEKLELCGLDPAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSIPGLE 420
Query: 1316 PTLAIHKDKLFFAPFGMDNSMEKDLCCDLHVSAYTSIKNL 1435
PTLAIHKDKLFFAPFGMDNSMEKDLCCDLHVSAYTSIKNL
Sbjct: 421 PTLAIHKDKLFFAPFGMDNSMEKDLCCDLHVSAYTSIKNL 460
>pir||T05899 hypothetical protein F6H11.180 - Arabidopsis
thaliana|gi|2827716|emb|CAA16689.1| predicted protein
[Arabidopsis thaliana]|gi|10177327|dbj|BAB10676.1|
contains similarity to unknown
protein~gene_id:MPA24.4~pir||T05899 [Arabidopsis
thaliana]
Length = 746
Score = 473 bits (1217), Expect = e-132
Identities = 247/293 (84%), Positives = 253/293 (86%)
Frame = +2
Query: 458 MVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELRRFTNKGKRNGLHVI 637
MVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELRRFTNKGKRNGLHVI
Sbjct: 1 MVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELRRFTNKGKRNGLHVI 60
Query: 638 HICTEMAPLVSVEPLASYITGLSCALQEEGYMVEVILPKYSTLDLDEIEGLREIEADAYS 817
HICTEMAPLVSV PLASYITGLSCALQEEGYMVEVILPKYSTLDLDEIEGLREIEADAYS
Sbjct: 61 HICTEMAPLVSVGPLASYITGLSCALQEEGYMVEVILPKYSTLDLDEIEGLREIEADAYS 120
Query: 818 YFDGQLHANRIWNGVVSGIGVTLIQPVYYSSMFSRDKVYGYQDDFDRFAYFSRASLDYIA 997
YFDGQLHANRIWN G+ + L + + S +K+ RFAYFSRASLDYIA
Sbjct: 121 YFDGQLHANRIWN----GLNLHLFSDWFGLYVLSENKI--------RFAYFSRASLDYIA 168
Query: 998 KSGKQPDVLHIHNWQTAIVGPLFWDVFVNQGLEGTRILLTCQDFDKGLVPPEKLELCGLD 1177
KSGKQPDVLHIHNWQTAIVGPLFWDVFVNQ GLVPPEKLELCGLD
Sbjct: 169 KSGKQPDVLHIHNWQTAIVGPLFWDVFVNQ----------------GLVPPEKLELCGLD 212
Query: 1178 PAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSIPGLEPTLAIHK 1336
PAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSIPGLEPTLAIHK
Sbjct: 213 PAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSIPGLEPTLAIHK 265
>gb|AAK98690.1|AC069158_2 Putative starch synthase [Oryza sativa]
Length = 720
Score = 383 bits (984), Expect = e-105
Identities = 208/400 (52%), Positives = 266/400 (66%), Gaps = 11/400 (2%)
Frame = +2
Query: 212 LGQSSITKEAKHK--DIWNLFREAQKNIMILNKQRLAAVXXXXXXXXXXXXXXXRINQLE 385
+ Q + + +H+ DIW LF +AQ+NI+ LNKQRL A+ I LE
Sbjct: 101 MSQETYLADERHEGVDIWELFSQAQRNILHLNKQRLVAMEELKKLQDENKSLLQDIEVLE 160
Query: 386 AESQ-IVIKKDKSSLFWELLLRIDSMVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQ 562
E Q I+ +SS F ELLLRID+M I+G+V + EAS +RK V ++ I D+
Sbjct: 161 TERQGFSIEIAESSRFSELLLRIDTMTISGMVGMTEASDLRKKVIDNRYMIHSVFSDIHH 220
Query: 563 QGDAEILAELRRFTNKGKRNGLHVIHICTEMAPLVSVEPLASYITGLSCALQEEGYMVEV 742
+ D E+L+ELR F K LHV+HIC+EM P+ S LA+Y+TGLS ALQ +G +VEV
Sbjct: 221 KQDTELLSELRLFLRKPAEKPLHVVHICSEMDPITSRGSLATYVTGLSSALQRKGNLVEV 280
Query: 743 ILPKYSTLDLDEIEGLREIEADAYSYFDGQLHANRIWNGVVSGIGVTLIQPVYYSSMFSR 922
ILPKY+ ++ D I LR+ EAD SY+ G H NRIW G+ SG+ +TLI+PV S F R
Sbjct: 281 ILPKYAGINEDVIHFLRKAEADYESYYGGCWHKNRIWTGISSGVSLTLIEPVQL-SYFDR 339
Query: 923 DKVYGYQDDFDRFAYFSRASLDYIAKSGKQPDVLHIHNWQTAIVGPLFWDVFVNQGLEGT 1102
D + GY DDF+RF+YFSRASLDYI KSGKQPDVLH+HNW+TAIVGPLFWD+F +QGL T
Sbjct: 340 DMLRGYPDDFERFSYFSRASLDYIVKSGKQPDVLHVHNWETAIVGPLFWDIFAHQGLGNT 399
Query: 1103 RILLTCQDF-DKGLVPPEKLELCGLDPAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVI 1279
RILLTCQD + L P LELCGLDP +LHR DRLQDN+ + VN+LKGG+VYSNKV++
Sbjct: 400 RILLTCQDLTSQCLEVPNMLELCGLDPHKLHRPDRLQDNSETNLVNVLKGGIVYSNKVLL 459
Query: 1280 MSSS-------HSSIPGLEPTLAIHKDKLFFAPFGMDNSM 1378
MSS+ GLE TL HK+K+ A G+D +
Sbjct: 460 MSSTLPRDMAIQGLGHGLEATLTTHKEKVLVASHGLDGEL 499
>emb|CAB40375.1| starch synthase, isoform V [Vigna unguiculata]
Length = 874
Score = 249 bits (636), Expect = 9e-65
Identities = 130/326 (39%), Positives = 196/326 (59%), Gaps = 9/326 (2%)
Frame = +2
Query: 428 FWE-LLLRIDSMVINGLVNIEEASSMRKLVKEHEANISVFPLDVLQQGDAEILAELRRFT 604
FW LLL ID + +++++A +R+ V + + ++S + ++ + E ++ T
Sbjct: 310 FWSRLLLLIDGWALEKKISVDDAKLLREKVWKRDKSVSDVYMAYKEKTEHEAISAFLGLT 369
Query: 605 NKGKRNGLHVIHICTEMAPLVSVEPLASYITGLSCALQEEGYMVEVILPKYSTLDLDEIE 784
+ GL+VIHI EMAP+ V L I+GLS ALQ++G++VE+ILPKY + D I
Sbjct: 370 SSATSPGLYVIHIAAEMAPVAKVGGLGDVISGLSKALQKKGHLVEIILPKYDCMQYDRIG 429
Query: 785 GLREIEADAYSYFDGQLHANRIWNGVVSGIGVTLIQPVYYSSMFSRDKVYGYQDDFDRFA 964
LR ++ SYFDGQL N+IW G V G+ V I+P + F R YG DDF RF+
Sbjct: 430 DLRALDVVIESYFDGQLFKNKIWVGTVEGLPVYFIEPHHPGKFFWRGDYYGAHDDFRRFS 489
Query: 965 YFSRASLDYIAKSGKQPDVLHIHNWQTAIVGPLFWDVFVNQGLEGTRILLTCQDFD-KGL 1141
YFSRA+L+++ ++GK+PD++H H+WQTA + PL+WDV+ +GL RI TC +F+ +G
Sbjct: 490 YFSRAALEFLLQAGKKPDIIHCHDWQTAFIAPLYWDVYAPKGLNSARICFTCHNFEYQGT 549
Query: 1142 VPPEKLELCGLDPAELHRLDRLQDNTNPHFVNILKGGVVYSNKVVIMSSSHSSI------ 1303
+LE CGLD +L+R DR+QDN+ + VN +KG VVYSN V +S +++
Sbjct: 550 AGASELEACGLDSHQLNRPDRMQDNSAHNRVNSVKGAVVYSNIVTTVSPTYAQEVRTAEG 609
Query: 1304 -PGLEPTLAIHKDKLFFAPFGMDNSM 1378
GL TL+ H K G+D +
Sbjct: 610 GKGLHSTLSTHSKKFIGILNGIDTDI 635
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 128
effective length of database: 257,790,653
effective search space used: 104663005118
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)