BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= PTA.CL.0.cl.013.Contig1
(2212 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_563764.1| cysteine proteinase; protein id: At1g06260... 300 e-147
emb|CAA06243.1| pre-pro-TPE4A protein [Pisum sativum] 137 9e-58
ref|NP_566633.1| cysteine proteinase; protein id: At3g19390... 135 1e-57
gb|AAL60580.1|AF454958_1 senescence-associated cysteine pro... 128 2e-57
sp|P12412|CYSP_VIGMU Vignain precursor (Bean endopeptidase)... 127 6e-57
>ref|NP_563764.1| cysteine proteinase; protein id: At1g06260.1 [Arabidopsis
thaliana]|gi|25289993|pir||D86198 cysteine proteinase (EC
3.4.22.-) [similarity] - Arabidopsis
thaliana|gi|8844131|gb|AAF80223.1|AC025290_12 Contains
similarity to a cysteine endopeptidase 1 from Phaseolus
vulgaris gb|U52970 and is a member of the papain cysteine
protease family PF|00112. [Arabidopsis thaliana]
Length = 343
Score = 300 bits (767), Expect(3) = e-147
Identities = 151/191 (79%), Positives = 152/191 (79%)
Frame = -1
Query: 1042 NCNKTQNLTLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWML 863
N + NLTLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWML
Sbjct: 3 NVLRNSNLTLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWML 62
Query: 862 RFGIYQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCD 683
RFGIYQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCD
Sbjct: 63 RFGIYQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCD 122
Query: 682 PAGNVPDAVDWRTQGAVTPIRNQGKCGMKLCFYIIHSKHKTLQTFIIQLMTF*LRFVFLV 503
PAGNVPDAVDWRTQGAVTPIRNQGKC
Sbjct: 123 PAGNVPDAVDWRTQGAVTPIRNQGKC---------------------------------- 148
Query: 502 AGGCWAFSAVA 470
GGCWAFSAVA
Sbjct: 149 -GGCWAFSAVA 158
Score = 189 bits (480), Expect(3) = e-147
Identities = 87/87 (100%), Positives = 87/87 (100%)
Frame = -3
Query: 341 PVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEE 162
PVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEE
Sbjct: 257 PVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEE 316
Query: 161 GYIRMERGVSEDTGKCGIAMMASYPLQ 81
GYIRMERGVSEDTGKCGIAMMASYPLQ
Sbjct: 317 GYIRMERGVSEDTGKCGIAMMASYPLQ 343
Score = 79.7 bits (195), Expect(3) = e-147
Identities = 36/36 (100%), Positives = 36/36 (100%)
Frame = -2
Query: 468 NKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGT 361
NKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGT
Sbjct: 188 NKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGT 223
>emb|CAA06243.1| pre-pro-TPE4A protein [Pisum sativum]
Length = 360
Score = 137 bits (344), Expect(3) = 9e-58
Identities = 62/88 (70%), Positives = 73/88 (82%), Gaps = 1/88 (1%)
Frame = -3
Query: 341 PVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQ-KYWIVKNSWGTGWGE 165
PVSV IDAGG+ FQ YS GVF+ +CGT+LNHGV VVGYGV D+ KYWIVKNSWG+ WGE
Sbjct: 257 PVSVAIDAGGYNFQFYSEGVFSGHCGTDLNHGVAVVGYGVTQDRTKYWIVKNSWGSEWGE 316
Query: 164 EGYIRMERGVSEDTGKCGIAMMASYPLQ 81
+GYIRM+RG+S G CGIAM ASYP++
Sbjct: 317 QGYIRMQRGISHKEGLCGIAMEASYPIK 344
Score = 81.3 bits (199), Expect(3) = 9e-58
Identities = 46/153 (30%), Positives = 78/153 (50%), Gaps = 5/153 (3%)
Frame = -1
Query: 1021 LTLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGIYQS 842
L ++ + + A+ + D K+L +E+W ++H + DE RF ++++
Sbjct: 7 LLFSLSLALIFRATNTFDFNEHDLDSEKSLWDLYERW-RSHHTVTRSLDEKHNRFNVFKA 65
Query: 841 NVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRP-----VCDPA 677
NV + N L P+KL N+FADMTN EF+ + S R+ + + +
Sbjct: 66 NVMHVHNTNKLDKPYKLKLNKFADMTNYEFRRIYADSKVSHHRMFRGMSNENGTFMYENV 125
Query: 676 GNVPDAVDWRTQGAVTPIRNQGKCGMKLCFYII 578
NVP ++DWR +GAVT +++QG+CG F I
Sbjct: 126 KNVPSSIDWRKKGAVTDVKDQGQCGSCWAFSTI 158
Score = 51.2 bits (121), Expect(3) = 9e-58
Identities = 23/36 (63%), Positives = 29/36 (79%)
Frame = -2
Query: 468 NKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGT 361
N+GC+GGLME AFEFIK N G+ TE++YPY +GT
Sbjct: 188 NEGCNGGLMEYAFEFIKQN-GITTESNYPYAAKDGT 222
>ref|NP_566633.1| cysteine proteinase; protein id: At3g19390.1, supported by cDNA:
gi_17065297, supported by cDNA: gi_20260003 [Arabidopsis
thaliana]|gi|11994461|dbj|BAB02463.1| cysteine
proteinase [Arabidopsis
thaliana]|gi|17065298|gb|AAL32803.1| cysteine proteinase
[Arabidopsis thaliana]|gi|20260004|gb|AAM13349.1|
cysteine proteinase [Arabidopsis thaliana]
Length = 452
Score = 135 bits (340), Expect(3) = 1e-57
Identities = 57/85 (67%), Positives = 69/85 (81%)
Frame = -3
Query: 341 PVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEE 162
P+SV I+AGG FQLY+SGVFT CGT+L+HGV VGYG EG Q YWIV+NSWG+ WGE
Sbjct: 260 PISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSNWGES 319
Query: 161 GYIRMERGVSEDTGKCGIAMMASYP 87
GY ++ER + E +GKCG+AMMASYP
Sbjct: 320 GYFKLERNIKESSGKCGVAMMASYP 344
Score = 84.0 bits (206), Expect(3) = 1e-57
Identities = 53/154 (34%), Positives = 89/154 (57%), Gaps = 4/154 (2%)
Frame = -1
Query: 1027 QNLTLAVLICFVLIAS-KLCSVDSSVYDPHKTLKQR-FEKWLKTHSKLYGGRDEWMLRFG 854
+++TLA+LI VL+ S L SV ++ ++ +R +E+WL + K Y G E RF
Sbjct: 6 KSITLALLIFSVLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLGEKERRFE 65
Query: 853 IYQSNVQLIDYINSL-HLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCDPA 677
I++ N++ ++ +S+ + +++ RFAD+TN EF+A +L R+ K
Sbjct: 66 IFKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMERTRVPVKGEKYLYKV 125
Query: 676 GN-VPDAVDWRTQGAVTPIRNQGKCGMKLCFYII 578
G+ +PDA+DWR +GAV P+++QG CG F I
Sbjct: 126 GDSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAI 159
Score = 49.7 bits (117), Expect(3) = 1e-57
Identities = 23/41 (56%), Positives = 28/41 (68%)
Frame = -2
Query: 468 NKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGTNRCSS 346
N GC GGLM+ AF+FI NGG+ TE DYPY + N C+S
Sbjct: 189 NDGCGGGLMDYAFKFIIENGGIDTEEDYPYIATD-VNVCNS 228
>gb|AAL60580.1|AF454958_1 senescence-associated cysteine protease [Brassica oleracea]
Length = 485
Score = 128 bits (321), Expect(3) = 2e-57
Identities = 54/87 (62%), Positives = 65/87 (74%)
Frame = -3
Query: 341 PVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGDQKYWIVKNSWGTGWGEE 162
P+SV I+ GG FQLY SG+F CGT+L+HGV VGYG E + YWIVKNSWGT WGE
Sbjct: 262 PISVAIEGGGRAFQLYDSGIFDGICGTDLDHGVVAVGYGTENGKDYWIVKNSWGTSWGES 321
Query: 161 GYIRMERGVSEDTGKCGIAMMASYPLQ 81
GYIRMER ++ GKCGIA+ SYP++
Sbjct: 322 GYIRMERNIASSAGKCGIAVEPSYPIK 348
Score = 80.9 bits (198), Expect(3) = 2e-57
Identities = 47/161 (29%), Positives = 75/161 (46%), Gaps = 9/161 (5%)
Frame = -1
Query: 1033 KTQNLTLAVLICFVLIASKLCSVDSSVYDP-HKTLKQR--------FEKWLKTHSKLYGG 881
K N +L +++ S + YD H T+ R +E+WL H K
Sbjct: 2 KLLNSATVILFLTMIVVSSAMDMSIISYDKNHHTVSSRSDAEVSRLYEEWLVKHGKAQNS 61
Query: 880 RDEWMLRFGIYQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKK 701
E RF I++ N++ ID N +L ++L +FAD+TN E+++ +LG
Sbjct: 62 LTEKDRRFEIFKDNLRFIDEHNGKNLSYRLGLTKFADLTNDEYRSMYLGSRLKRKATKSS 121
Query: 700 QRPVCDPAGNVPDAVDWRTQGAVTPIRNQGKCGMKLCFYII 578
R +P++VDWR +GAV +++QG CG F I
Sbjct: 122 LRYEVRVGDAIPESVDWRKEGAVAEVKDQGSCGSCWAFSTI 162
Score = 59.3 bits (142), Expect(3) = 2e-57
Identities = 26/42 (61%), Positives = 32/42 (75%)
Frame = -2
Query: 468 NKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGTNRCSST 343
N+GC+GGLM+ AFEFI NGG+ TE DYPY G++G RC T
Sbjct: 192 NEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGVDG--RCDQT 231
>sp|P12412|CYSP_VIGMU Vignain precursor (Bean endopeptidase) (Cysteine proteinase)
(Sulfhydryl-endopeptidase) (SH-EP)|gi|82006|pir||S12581
cysteine proteinase (EC 3.4.22.-) precursor - black
gram|gi|22062|emb|CAA33753.1|
sulfhydryl-pre-endopeptidase (AA -20 to 342) [Vigna
mungo]|gi|22066|emb|CAA36181.1| sulfhydryl-endopeptidase
[Vigna mungo]
Length = 362
Score = 127 bits (319), Expect(3) = 6e-57
Identities = 59/94 (62%), Positives = 70/94 (73%), Gaps = 1/94 (1%)
Frame = -3
Query: 341 PVSVGIDAGGFIFQLYSSGVFTNYCGTNLNHGVTVVGYGVEGD-QKYWIVKNSWGTGWGE 165
PVSV IDAGG FQ YS GVFT C T+LNHGV +VGYG D YWIV+NSWG WGE
Sbjct: 258 PVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTTVDGTNYWIVRNSWGPEWGE 317
Query: 164 EGYIRMERGVSEDTGKCGIAMMASYPLQ*VHESP 63
+GYIRM+R +S+ G CGIAMMASYP++ ++P
Sbjct: 318 QGYIRMQRNISKKEGLCGIAMMASYPIKNSSDNP 351
Score = 78.6 bits (192), Expect(3) = 6e-57
Identities = 41/129 (31%), Positives = 72/129 (55%), Gaps = 5/129 (3%)
Frame = -1
Query: 949 DPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGIYQSNVQLIDYINSLHLPFKLTDNRFAD 770
+ ++L +E+W ++H + E RF ++++NV + N + P+KL N+FAD
Sbjct: 31 ESEESLWDLYERW-RSHHTVSRSLGEKHKRFNVFKANVMHVHNTNKMDKPYKLKLNKFAD 89
Query: 769 MTNSEFKAHFLGLNTSSLRL-----HKKQRPVCDPAGNVPDAVDWRTQGAVTPIRNQGKC 605
MTN EF++ + G + ++ H + + G+VP +VDWR +GAVT +++QG+C
Sbjct: 90 MTNHEFRSTYAGSKVNHHKMFRGSQHGSGTFMYEKVGSVPASVDWRKKGAVTDVKDQGQC 149
Query: 604 GMKLCFYII 578
G F I
Sbjct: 150 GSCWAFSTI 158
Score = 60.8 bits (146), Expect(3) = 6e-57
Identities = 25/37 (67%), Positives = 32/37 (85%)
Frame = -2
Query: 471 QNKGCSGGLMETAFEFIKTNGGLATETDYPYTGIEGT 361
+N+GC+GGLME+AFEFIK GG+ TE++YPYT EGT
Sbjct: 187 ENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGT 223
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 131
effective length of database: 253,773,515
effective search space used: 153532976575
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)