BLASTX 1.4.13-Paracel [2002-12-12]
Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AV534702.r
(592 letters)
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
1,339,046 sequences; 429,188,541 total letters
Searching...................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_563764.1| cysteine proteinase; protein id: At1g06260... 409 e-113
pir||S24602 cysteine proteinase tpp (EC 3.4.22.-) - garden ... 171 8e-42
pir||T12041 cysteine proteinase (EC 3.4.22.-) 3 precursor -... 169 3e-41
gb|AAK48495.1|AF259983_1 putative cysteine protease [Ipomoe... 163 1e-39
ref|NP_566633.1| cysteine proteinase; protein id: At3g19390... 162 2e-39
>ref|NP_563764.1| cysteine proteinase; protein id: At1g06260.1 [Arabidopsis
thaliana]|gi|25289993|pir||D86198 cysteine proteinase
(EC 3.4.22.-) [similarity] - Arabidopsis
thaliana|gi|8844131|gb|AAF80223.1|AC025290_12 Contains
similarity to a cysteine endopeptidase 1 from Phaseolus
vulgaris gb|U52970 and is a member of the papain
cysteine protease family PF|00112. [Arabidopsis
thaliana]
Length = 343
Score = 409 bits (1051), Expect = e-113
Identities = 197/197 (100%), Positives = 197/197 (100%)
Frame = +1
Query: 1 NSNLTLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGI 180
NSNLTLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGI
Sbjct: 7 NSNLTLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGI 66
Query: 181 YQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCDPAGN 360
YQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCDPAGN
Sbjct: 67 YQSNVQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCDPAGN 126
Query: 361 VPDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDCDVGT 540
VPDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDCDVGT
Sbjct: 127 VPDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDCDVGT 186
Query: 541 YNKGCSGGLMETAFEFI 591
YNKGCSGGLMETAFEFI
Sbjct: 187 YNKGCSGGLMETAFEFI 203
>pir||S24602 cysteine proteinase tpp (EC 3.4.22.-) - garden
pea|gi|3980198|emb|CAA46863.1| thiolprotease [Pisum
sativum]
Length = 464
Score = 171 bits (432), Expect = 8e-42
Identities = 86/198 (43%), Positives = 125/198 (62%), Gaps = 5/198 (2%)
Frame = +1
Query: 13 TLAVLICFVLIASKLCSVDSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGIYQSN 192
TL++ + +I+ D S + + +E+WL H K Y E RF I++ N
Sbjct: 15 TLSLALDMCIISYDKTHPDKSTPRTNDQVLTMYEEWLVKHGKNYNALGEKEKRFEIFKDN 74
Query: 193 VQLIDYINSLHLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKK-----QRPVCDPAG 357
+ ID NS +L F+L NRFAD+TN E++ FLG + R ++K R
Sbjct: 75 LGFIDEHNSKNLSFRLGLNRFADLTNEEYRTRFLGTRINPNRRNRKVNSQTNRYATRVGD 134
Query: 358 NVPDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDCDVG 537
+P++VDWR +GAV +++QG CG CWAFSA+AA+EG+NK+ TG+L+SLSEQ+L+DCD
Sbjct: 135 KLPESVDWRKEGAVVGVKDQGSCGSCWAFSAIAAVEGVNKLATGDLISLSEQELVDCDT- 193
Query: 538 TYNKGCSGGLMETAFEFI 591
+YN+GC+GGLM+ AFEFI
Sbjct: 194 SYNEGCNGGLMDYAFEFI 211
>pir||T12041 cysteine proteinase (EC 3.4.22.-) 3 precursor - kidney
bean|gi|2511693|emb|CAB17076.1| cysteine proteinase
precursor [Phaseolus vulgaris]
Length = 455
Score = 169 bits (427), Expect = 3e-41
Identities = 84/178 (47%), Positives = 117/178 (65%), Gaps = 3/178 (1%)
Frame = +1
Query: 67 DSSVYDPHKTLKQRFEKWLKTHSKLYGGRDEWMLRFGIYQSNVQLIDYINSLHLPFKLTD 246
D + + + + +E+WL H KLY E RF I++ N++ ID N+ + +KL
Sbjct: 26 DKATWRTDEEVNSLYEEWLVKHGKLYNALGEKDKRFQIFKDNLRFIDQQNAENRTYKLGL 85
Query: 247 NRFADMTNSEFKAHFLGLNTS-SLRLHKKQRPVCDP--AGNVPDAVDWRTQGAVTPIRNQ 417
NRFAD+TN E++A +LG + RL + P +PD+VDWR +GAV P+++Q
Sbjct: 86 NRFADLTNEEYRARYLGTKIDPNRRLGRTPSNRYAPRVGETLPDSVDWRKEGAVVPVKDQ 145
Query: 418 GKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDCDVGTYNKGCSGGLMETAFEFI 591
CG CWAFSA+ A+EGINKI TG+L+SLSEQ+L+DCD G YN GC+GGLM+ AFEFI
Sbjct: 146 ASCGSCWAFSAIGAVEGINKIVTGDLISLSEQELVDCDTG-YNMGCNGGLMDYAFEFI 202
>gb|AAK48495.1|AF259983_1 putative cysteine protease [Ipomoea batatas]
Length = 462
Score = 163 bits (413), Expect = 1e-39
Identities = 96/212 (45%), Positives = 132/212 (61%), Gaps = 20/212 (9%)
Frame = +1
Query: 16 LAVLICFVL--IASKLCSVDSSV--YD---PHKTLKQR-------FEKWLKTHSKLYGGR 153
L +L FVL ++S S D S+ YD P K L + +E WL H K Y G
Sbjct: 4 LLILSLFVLAAVSSASASADMSIITYDEEHPAKGLSRSDEEVMALYESWLVEHGKSYNGL 63
Query: 154 D-EWMLRFGIYQSNVQLIDYINSL-HLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHK 327
E RF I++ N++ ID NS +KL NRFAD+TN E+++ +LG T + R
Sbjct: 64 GGEKDKRFEIFKDNLRYIDEQNSRGDRSYKLGLNRFADLTNEEYRSTYLGAKTDARRRIA 123
Query: 328 K----QRPVCDPAGNVPDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNL 495
K +R G++PD++DWR +GAV +++QG CG CWAFS +AA+EGIN+I TG L
Sbjct: 124 KTKSDRRYAPKAGGSLPDSIDWREKGAVAEVKDQGSCGSCWAFSTIAAVEGINQIVTGEL 183
Query: 496 VSLSEQQLIDCDVGTYNKGCSGGLMETAFEFI 591
+SLSEQ+L+DCD +YN+GC+GGLM+ AFEFI
Sbjct: 184 ISLSEQELVDCDT-SYNEGCNGGLMDYAFEFI 214
>ref|NP_566633.1| cysteine proteinase; protein id: At3g19390.1, supported by cDNA:
gi_17065297, supported by cDNA: gi_20260003 [Arabidopsis
thaliana]|gi|11994461|dbj|BAB02463.1| cysteine
proteinase [Arabidopsis
thaliana]|gi|17065298|gb|AAL32803.1| cysteine proteinase
[Arabidopsis thaliana]|gi|20260004|gb|AAM13349.1|
cysteine proteinase [Arabidopsis thaliana]
Length = 452
Score = 162 bits (411), Expect = 2e-39
Identities = 88/199 (44%), Positives = 132/199 (66%), Gaps = 4/199 (2%)
Frame = +1
Query: 7 NLTLAVLICFVLIAS-KLCSVDSSVYDPHKTLKQR-FEKWLKTHSKLYGGRDEWMLRFGI 180
++TLA+LI VL+ S L SV ++ ++ +R +E+WL + K Y G E RF I
Sbjct: 7 SITLALLIFSVLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLGEKERRFEI 66
Query: 181 YQSNVQLIDYINSL-HLPFKLTDNRFADMTNSEFKAHFLGLNTSSLRLHKKQRPVCDPAG 357
++ N++ ++ +S+ + +++ RFAD+TN EF+A +L R+ K G
Sbjct: 67 FKDNLKFVEEHSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKMERTRVPVKGEKYLYKVG 126
Query: 358 N-VPDAVDWRTQGAVTPIRNQGKCGGCWAFSAVAAIEGINKIKTGNLVSLSEQQLIDCDV 534
+ +PDA+DWR +GAV P+++QG CG CWAFSA+ A+EGIN+IKTG L+SLSEQ+L+DCD
Sbjct: 127 DSLPDAIDWRAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISLSEQELVDCDT 186
Query: 535 GTYNKGCSGGLMETAFEFI 591
+YN GC GGLM+ AF+FI
Sbjct: 187 -SYNDGCGGGLMDYAFKFI 204
Database: BlastDB/NCBI/blast/db/2003_02_17_02_00_1/nr
Posted date: Feb 17, 2003 10:02 AM
Number of letters in database: 429,188,541
Number of sequences in database: 1,339,046
Lambda K H
0.318 0.135 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
length of database: 429,188,541
effective HSP length: 117
effective length of database: 272,520,159
effective search space used: 21529092561
frameshift window, decay const: 50, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)